LOCUS       sequence-445621-        2639 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses untagged ATG3 (Y209A) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Rao S, Skulsuppaisarn M, Strong LM, Ren X, Lazarou M, Hurley JH, 
            Hummer G
  TITLE     Three-step docking by WIPI2, ATG16L1, and ATG3 delivers LC3 to the 
            phagophore.
  JOURNAL   Sci Adv. 2024 Feb 9;10(6):eadj8027. doi: 10.1126/sciadv.adj8027. 
            Epub 2024 Feb 7.
  PUBMED    38324698
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-02-9"; volume: "10(6):eadj8027. doi"; pages: " 
            10.1126/sciadv.adj8027. Epub 2024 Feb 7"
COMMENT     Sequence Label: pMX-IG-ATG3 (Y209A)
FEATURES             Location/Qualifiers
     source          1..2639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      22..610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     511..530
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     764..781
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             911..1380
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        1532..1636
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1637..1705,1706..2497)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1855..1874)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
       61 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
      121 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
      181 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
      241 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      301 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      361 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      421 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      481 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      541 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      601 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      661 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      721 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      781 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      841 atgcaactat ggccatttaa tgtaaatact taagaaaaaa aaccaaatta attttgatac
      901 atgctgcatg tgaaagaccc ccgctgacgg gtagtcaatc actcagagga gaccctccca
      961 aggaacagcg agaccacaag tcggatgcaa ctgcaagagg gtttattgga tacacgggta
     1021 cccgggcgac tcagtcaatc ggaggactgg cgccccgagt gaggggttgt gggctctttt
     1081 attgagctcg gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt
     1141 caaataaggc acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct
     1201 ctagaaactg ctgagggctg gaccgcatct ggggaccatc tgttcttggc cctgagccgg
     1261 ggcaggaact gcttaccaca gatatcctgt ttggcccata ttcagctgtt ccatctgttc
     1321 ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag
     1381 ctgttccatc tgttcctgac cttgatctga acttttctat tctcagttat gtatttttcc
     1441 atgccttgca aaatggcgtt acttaagcta gcttgccaaa cctacaggtg gggtctttca
     1501 ttatgcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1561 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1621 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1681 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1741 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1801 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1861 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1921 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1981 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     2041 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     2101 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     2161 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     2221 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     2281 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2341 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2401 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2461 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2521 tatactttag attgatttgc ggccggccgc aaaaggatct aggtgaagat cctttttgat
     2581 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgt
//
