LOCUS       sequence-445647-       13099 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Donor plasmid of MAG Transposon with 10 kb cargo gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13099)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 13099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pQLL-MAG-10 kb
FEATURES             Location/Qualifiers
     source          1..13099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    228..309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(265..284)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1852..1875)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      1875..1884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1899..3077)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1908..2846
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(1931..1951)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     polyA_signal    3123..3347
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3336..3353
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(3375..4088,4089..4091,4092..4094))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3378..4088,4089..4091,4092..4094))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3420..3441)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3769..3788
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4028..4049
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(4095..4151)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4158..4757)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4238..4258)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     regulatory      4754..4763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4777..5276)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4825..4844)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5218..5241
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(5972..5989)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5972..5988)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5998..6016)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6084..6188
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6189..6257,6258..7049)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6407..6426)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7220..7808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7709..7728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7962..7979
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7991..8007
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7991..8007
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8458..8852
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
ORIGIN
        1 tattaatatg aaacaaggat atggaagagg aaggcatgaa aacaggaaaa gaaaacaaac
       61 cttgtttgcc attttaaggc acccctggac agctaggtgg caaaaggaag cttccctttg
      121 gtctccccct cttaagtacc acatttgtag aggttttact tgctttaaaa aacctcccac
      181 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
      241 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
      301 tttcactgca ttctagttgt ggtttgtcca aactcaatcg atgcgagggg gcagggcctg
      361 catgtgaagg gcgtcgtagg tgtccttggt ggctgtactg agaccctggt aaaggccatc
      421 gtgccccttg cccctccggc gctcgccttt catcccaatc tcactgtagg cctccgccat
      481 cttatctttc tgcagttcat tgtacaggcc ttcctgaggg ttcttccttc tcggctttcc
      541 ccccatctca gggtcccggc cacgtctctt gtccaaaaca tcgtactcct ctcttcgtcc
      601 tagattgagc tcgttataga gctggttctg gccctgctgg tacgcggggg cgtctgcgct
      661 cctgctgaac ttcactctgg agcgataggc tgcgaagtcg cgtggtgggg catagggctg
      721 gtaatgcttg cgggtgggcc cggggcggcg gggagtcatg ttcatgtagt cactgtgcag
      781 gagcctgctc ctcttactcc tcacccagaa aataataaag gccactgtta ctagcaagct
      841 atagcaagcc aggactcccc caaccaccac cagcacccaa aaatcacagg cgaagtccag
      901 ccccctcgtg tgcactgcgc cccccgccgc tggccggcac gcctctgggc gcagggacag
      961 gggctgcgac gcgatggtgg gcgccggtgt tggtggtcgc ggcgctggcg tcgtggtcag
     1021 cactgtcagt tgcgtgcctc caccgaaaac agcggctgaa ctatgccaaa tcatacagta
     1081 ataatcggcc tcgtcctcgg acctgagacc tgagatgagc agcacacccg cattggcgct
     1141 tgcgtcttta gatccactaa accttgatgg aactccagat ccctgctgct tatctgagtc
     1201 gctcttgtaa ttgagaaggt attggggagg agaacctggt ttttgctgat accagtagat
     1261 cctgtaaggc cctacattga ttcctgacct cagtgtacac gtcaaagaag cggaggcacc
     1321 ggggcttgca ctgagggagg atgattgcgt gagaacaggt tggctacccc cccctccgga
     1381 cccgccccca ccggaacctc caccgcctga gcccaggatc ccactggaca cggtgacggt
     1441 tgtaccttgt ccccaaacgt ccataccata gtagtaagtc atcatacccc gcgcacaata
     1501 gtaaacagct gtgtcttcgg gagttacact attaagctga agagagaact ggtttttgga
     1561 agtatctgga tttatagaca tcctgctttt cacacttaca gcataatcat tataccattt
     1621 actgcggtag tatgttctac cgagccattc caaccctcga ctgggggact gcctgatcca
     1681 attccatgtc gcggagttac tactcactga atcacctgat atggcacagg taagactaag
     1741 agtctgggaa ggagtgacca gaccggggcc agattgttgg agttgcactt gcggcctggc
     1801 ggcgtggagc agcaaggcca gcggcaggag caaggcggtc actggtaagg ccttatcgtc
     1861 atcgtctttg taatccatgg tggcgaccgg tggatccctc acgacacctg aaatggaaga
     1921 aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc aagatccaaa ctcaaaaagg
     1981 gcaaattcca aggagaatta catcaagtgc caagctggcc taacttcagt ctccacccac
     2041 tcagtgtggg gaaactccat cgcataaaac ccctcccccc aacctaaaga cgacgtactc
     2101 caaaagctcg agaactaatc gaggtgcctg gacggcgccc ggtactcagt ggagtcacat
     2161 gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg tgggtgactc acccgcccgc
     2221 tctcccgagc gccgcgtcct ccattttgag ctccctgcag cagggccggg aagcggccat
     2281 ctttccgctc acgcaactgg tgccgaccgg gccagccttg ccgcccaggg cggggcgata
     2341 cacggcggcg cgaggccagg caccagagca ggccggcgag cttgagacta cccccgtccg
     2401 attctcggtg gccgcgctcg caggccccgc ctcgccgaac atgtgcgctg ggacgcacgg
     2461 gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag tgtgcagatc ttggcccgca
     2521 tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag gtcatcaaaa attttaaatg
     2581 gctagagact tatcgaaagc agcgagacag gcgcgaaggt gccaccagat tcgcacgcgg
     2641 cggccccagc gcccaggcca ggcctcaact caagcacgag gcgaaggggc tccttaagcg
     2701 caaggcctcg aactctccca cccacttcca acccgaagct cgggatcaag aatcacgtac
     2761 tgcagccagg tggaagtaat tcaaggcacg caagggccat aacccgtaaa gaggccaggc
     2821 ccgcgggaac cacacacggc acttacctgt gttctggcgg caaacccgtt gcgaaaaaga
     2881 acgttcacgg cgactactgc acttatatac ggttctcccc caccctcggg aaaaaggcgg
     2941 agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta ggcaccgggt
     3001 caattgccga cccctccccc caacttctcg gggactgtgg gcgatgtgcg ctctgcccac
     3061 tgacgggcac cggagcctca cgttaattaa ccaaggtacc cattggcggc cgccctcaga
     3121 agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt
     3181 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
     3241 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
     3301 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca
     3361 gcgagcttaa ttaattactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
     3421 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
     3481 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
     3541 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
     3601 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
     3661 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
     3721 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
     3781 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
     3841 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc
     3901 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
     3961 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
     4021 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
     4081 cccttgctca ccattggccc gggattctct tcgacatccc ctgcttgttt caacagggag
     4141 aagttagtgg catcgatggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg
     4201 ggcacctcga cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg
     4261 ggcgcggagg tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc cactccgggg
     4321 agcacgacgg cgctgcccag acccttgccc tggtggtcgg gcgagacgcc gacggtggcc
     4381 aggaaccacg cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc
     4441 gcggccagcc gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc
     4501 cccgcttcga cgctctccgg cgtggtccag accgccaccg cggcgccgtc gtccgcgacc
     4561 cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc
     4621 cgctcgatgt ggcggtccgg gtcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg
     4681 gcggcgaggg tgcgtacggc ccgggggacg tcgtcgcggg tggcgaggcg caccgtgggc
     4741 ttgtactcgg tccccatggt ggcgttggct gcaggtcgaa aggcccggag atgaggaaga
     4801 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     4861 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     4921 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     4981 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     5041 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     5101 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     5161 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     5221 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     5281 gaattgctag caccggtcgt tggaattcct tcaatcaagc gcttgaagta gtgcacccaa
     5341 aaatacacga gctaatggat cgtgtgaagt gttctaggca acggcttaga acacagtgta
     5401 agcgatccag cgctgtgacg cgcttttata cccatagacg aaggtactca ccactatttc
     5461 gtctctcagt gcaaagggag ctcaaaaccc ttaagcgagt gccgtaatat tcttgcatta
     5521 gtaaattaga gcatgttaat taaagacaca gcgaattgcc actgcgctaa gcgtttctac
     5581 aaaaaaaatg aatacgcatt agttcactaa tttaagttaa gttatccgac aactcaataa
     5641 catcaaaaac tgtgttttaa caataataaa tgataattat tttaaaattt tctgaactgg
     5701 tcctattatt ttggcgcatg atttttgggt tatttttaca tttcttatca taactttttt
     5761 gtttcttaac ttttcaccaa actttaaaaa cattcttaaa gtaaacattc taatagtgct
     5821 ttcatgaaag tttggtaaag attgattcac tttaagtcat gaaatatgat aagtgaataa
     5881 taaattgata agtggtccta ttgatttgtc cgacactgta tacaattgag agacgcaaga
     5941 cactgcggat cgagacgcat tgcgtggttc gactggccgt cgttttacaa cgaaagggcc
     6001 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     6061 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6121 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6181 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6241 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6301 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6361 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6421 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6481 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6541 gagaattatg tagtgctgcc ataaccatga gtgataacac ggcggccaac ttacttctga
     6601 caacgatcgg aggaccgaag gagctaaccg cttttttgcg caacatgggg gatcatgtaa
     6661 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgata
     6721 ccacgatgcc tgtagcgatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6781 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6841 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6901 gtgggtcccg cggtattatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6961 ttatctacac gacggggagt caggcaacta tggatgaacg aaagagacag atcgctgaga
     7021 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7081 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7141 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7201 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7261 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7321 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7381 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7441 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7501 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7561 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7621 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7681 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7741 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7801 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7861 ctcacatgtt ctttcctgcg ttatcccctg attctgtggg taaccgtatt accgcctttg
     7921 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7981 aagcggcaca caggaaacag ctatgaccgt ctcggttggc agtgactccg tctctgggcc
     8041 ctatacagtg tcggacaaat caataggacc acttaataat gttttattca cttatcacat
     8101 ttcattacct aaagtgaatc aatatttacc aaattttcat gaaagcactt ttcggatgtt
     8161 ggcttaaagt ctgctttgaa agtttgttga taagttaaca aacaaaaaag ttattataag
     8221 aaatgttgaa aaaaccccaa aaatcatgcg acaaaataat aggaccagta tagaaaattt
     8281 tattatttta accatttatc attattgcct ataaataatt acagctccgg cttttaattc
     8341 gcaatgtggc cagcccgtac atttgaaaaa ctaaagaaaa taacaaaaca gccttatcct
     8401 gggctaactt ggtagcatct cgagggccca tgaggacagc taaaacaata agtaatgtaa
     8461 aatacagcat agcaaaactt taacctccaa atcaagcctc tacttgaatc cttttctgag
     8521 ggatgaataa ggcataggca tcaggggctg ttgccaatgt gcattagctg tttgcagcct
     8581 caccttcttt catggagttt aagatatagt gtattttccc aaggtttgaa ctagctcttc
     8641 atttctttat gttttaaatg cactgacctc ccacattccc tttttagtaa aatattcaga
     8701 aataatttaa atacatcatt gcaatgaaaa taaatgtttt ttattaggca gaatccagat
     8761 gctcaaggcc cttcataata tcccccagtt tagtagttgg acttagggaa caaaggaacc
     8821 tttaatagaa attggacagc aagaaagcga gcttagtgat acttgtgggc cagggcatta
     8881 gccacaccag ccaccacttt ctgataggca gcctgcactg gtggggtgaa ttctttgcca
     8941 aagtgatggg ccagcacaca gaccagcacg ttgcccagga gctgtgggag gaagataaga
     9001 ggtatgaaca tgattagcaa aagggcctag cttggactca gaataatcca gccttatccc
     9061 aaccataaaa taaaagcaga atggtagctg gattgtagct gctattagca atatgaaacc
     9121 tcttacatca gttacaattt atatgcagaa atatttatat gcagaaatat tgctattgcc
     9181 ttaacccaga aattatcact gttattcttt agaatggtgc aaagaggcat gatacattgt
     9241 atcattattg ccctgaaaga aagagattag ggaaagtatt agaaataaga taaacaaaaa
     9301 agtatattaa aagaagaaag cattttttaa aattacaaat gcaaaattac cctgatttgg
     9361 tcaatatgtg tacacatatt aaaacattac actttaaccc ataaatatgt ataatgatta
     9421 tgtatcaatt aaaaataaaa gaaaataaag tagggagatt atgaatatgc aaataagcac
     9481 acatatattc caaatagtaa tgtactaggc agactgtgta aagttttttt ttaagttact
     9541 taatgtatct cagagatatt tccttttgtt atacacaatg ttaaggcatt aagtataata
     9601 gtaaaaattg cggagaagaa aaaaaaagaa agcaagaatt aaacaaaaga aaacaattgt
     9661 tatgaacagc aaataaaaga aactaaaacg atcctgagac ttccacactg atgcaatcat
     9721 tcgtctgttt cccattctaa actgtaccct gttacttctc cccttcctat gacatgaact
     9781 taaccataga aaagaagggg aaagaaaaca tcaagggtcc catagactca ccctgaagtt
     9841 ctcaggatcc acgtgcagct tgtcacagtg cagctcactc agtgtggcaa aggtgccctt
     9901 gaggttgtcc aggtgagcca ggccatcact aaaggcaccg agcactttct tgccatgagc
     9961 cttcacctta gggttgccca taacagcatc aggagtggac agatccccaa aggactcaaa
    10021 gaacctctgg gtccaagggt agaccaccag cagcctaagg gtgggaaaat agaccaatag
    10081 gcagagagag tcagtgccta tcagaaaccc aagagtcttc tctgtctcca catgcccagt
    10141 ttctattggt ctccttaaac ctgtcttgta accttgatac caacctgccc agggcctcac
    10201 caccaacttc atccacgttc accttgcccc acagggcagt aacggcagac ttctcctcag
    10261 gagtcaggtg caccatggtg tctgtttgag gttgctagtg aacacagttg tgtcagaagc
    10321 aaatgtaagc aatagatggc tctgccctga cttttatgcc cagccctggc tcctgccctc
    10381 cctgctcctg ggagtagatt ggccaaccct agggtgtggc tccacagggt gaggtctaag
    10441 tgatgacagc cgtacctgtc cttggctctt ctggcactgg cttaggagtt ggacttcaaa
    10501 ccctcagccc tccctctaag atatatctct tggccccata ccatcagtac aaattgctac
    10561 taaaaacatc ctcctttgca agtgtattta cgcttctttg agaaacatct tcttcgttag
    10621 tggcctgccc ctcattccca ctttaatatc cagaatcact ataagaagaa tataataaga
    10681 ggaataactc ttattatagg taagggaaaa ttaagaggca tacgtgatgg gatgagtaag
    10741 agaggagagg gaaggattaa tggatgataa aatctactac tatttgttga gaccttttat
    10801 agtctaatca attttgctat tgttttccat cctcacgcta actccataaa aaaacactat
    10861 tattatcttt attttgccat gacaagactg agctcagaag agtcaagcat ttgcctaagg
    10921 tcggacatgt cagaggcagt gccagaccta tgtgagactc tgcagctact gctcatgggc
    10981 cctgtgctgc actgatgagg aggatcagat ggatggggca atgaagcaaa ggaatcattc
    11041 tgtggataaa ggagacagcc atgaagaagt ctatgactgt aaatttggga gcaggagtct
    11101 ctaaggactt ggatttcaag gaattttgac tcagcaaaca caagaccctc acggtgactt
    11161 tgcgagctgg tgtgccagat gtgtctatca gaggttccag ggagggtggg gtggggtcag
    11221 ggctggccac cagctatcag ggcccagatg ggttataggc tggcaggctc agataggtgg
    11281 ttaggtcagg ttggtggtgc tgggtggagt ccatgactcc caggagccag gagagataga
    11341 ccatgagtag agggcagaca tgggaaaggt gggggaggca cagcatagca gcatttttca
    11401 ttctactact acatgggact gctcccctat acccccagct aggggcaagt gccttgactc
    11461 ctatgttttc aggatcatca tctataaagt aagagtaata attgtgtcta tctcataggg
    11521 ttattatgag gatcaaagga gatgcacact ctctggacca gtggcctaac agttcaggac
    11581 agagctatgg gcttcctatg tatgggtcag tggtctcaat gtagcaggca agttccagaa
    11641 gatagcatca accactgtta gagatatact gccagtctca gagcctgatg ttaatttagc
    11701 aatgggctgg gaccctcctc cagtagaacc ttctaaccag ctgctgcagt caaagtcgaa
    11761 tgcagctggt tagacttttt ttaatgaaag cttgtaccag tggggcctct aagactaagt
    11821 cactctgtct caccgtgtct tagccagttc cttacagctt gccctgatgg gagatagaga
    11881 atgggtatcc tccaacaaaa aaataaattt tcatttctca aggtccaact tatgttttct
    11941 taatttttaa aaaaatcttg accattctcc actctctaaa ataatccaca gtgagagaaa
    12001 cattcttttc ccccatccca taaatacctc tattaaatat ggaaaatctg ggcatggtgt
    12061 ctcacacctg taatcccagc actttgggag gctgaggtgg gtggactgct tggagctcag
    12121 gagttcaaga ccatcttgga caacatggtg ataccctgcc tctacaaaaa gtacaaaaat
    12181 tagcctggca tggtggtgtg cacctgtaat cccagctatt agggtggctg aggcaggaga
    12241 attgcttgaa cccgggaggc ggaggttgca gtgagctgag atcgtgccac tgcactccag
    12301 cctgggggac agagcacatt ataattaact gttatttttt acttggactc ttgtggggaa
    12361 taagatacat gttttattct tatttatgat tcaagcactg aaaatagtgt ttagcatcca
    12421 gcaggtgctt caaaaccatt tgctgaatga ttactatact ttttacaagc tcagctccct
    12481 ctatcccttc cagcatcctc atctctgatt aaataagctt cagtttttcc ttagttcctg
    12541 ttacatttct gtgtgtctcc attagtgacc tcccatagtc caagcatgag cagttctggc
    12601 caggcccctg tcggggtcag tgccccaccc ccgccttctg gttctgtgta accttctaag
    12661 caaaccttct ggctcaagca cagcaatgct gagtcatgat gagtcatgct gaggcttagg
    12721 gtgtgtgccc agatgttctc agcctagagt gatgactcct atctgggtcc ccagcaggat
    12781 gcttacaggg cagatggcaa aaaaaaggag aagctgacca cctgactaaa actccacctc
    12841 aaacggcatc ataaagaaaa tggatgcctg agacagaatg tgacatattc tagaatatat
    12901 tatttcctga atatatatat atatatatat acatatacgt atatatatat atatatatat
    12961 atttgttgtt atcaattgcc atagaatgat tagttattgt gaatcaaata tttatcttgc
    13021 aggtggcctc tatacctaga agcggcagaa tcaggcttta ttaatacatg tgtatagatt
    13081 tttaggatct atacacatg
//
