LOCUS       sequence-445652-        9662 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Donor plasmid of MAG Transposon with 5 kb cargo gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pQLL-MAG-5 kb
FEATURES             Location/Qualifiers
     source          1..9662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      348..357
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(371..870)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(419..438)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     812..835
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(1566..1583)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1566..1582)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1592..1610)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3016..3120
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3121..3189,3190..3981)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3339..3358)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4152..4740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4641..4660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4894..4911
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4923..4939
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4923..4939
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5484..5565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5521..5540)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7108..7131)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7131..7140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7155..8333)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7164..8102
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(7187..7207)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     polyA_signal    8379..8603
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8592..8609
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(8631..9344,9345..9347,9348..9350))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8634..9344,9345..9347,9348..9350))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8676..8697)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9025..9044
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9284..9305
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(9351..9407)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(join(9414..9662,1..351))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9494..9514)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc
       61 ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt
      121 ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac
      181 gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt ccgggtcgac
      241 ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggcccgggg
      301 gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcccca tggtggcgtt
      361 ggctgcaggt cgaaaggccc ggagatgagg aagaggagaa cagcgcggca gacgtgcgct
      421 tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga
      481 gcccgcccct gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga
      541 aggagcaaag ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg
      601 tgctgtccat ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg
      661 acgcgagctg cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga
      721 aggggccacc aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag
      781 gccagaggcc acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag
      841 actgccttgg gaaaagcgcc tcccctaccc ggtagaattg ctagcaccgg tcgttggaat
      901 tccttcaatc aagcgcttga agtagtgcac ccaaaaatac acgagctaat ggatcgtgtg
      961 aagtgttcta ggcaacggct tagaacacag tgtaagcgat ccagcgctgt gacgcgcttt
     1021 tatacccata gacgaaggta ctcaccacta tttcgtctct cagtgcaaag ggagctcaaa
     1081 acccttaagc gagtgccgta atattcttgc attagtaaat tagagcatgt taattaaaga
     1141 cacagcgaat tgccactgcg ctaagcgttt ctacaaaaaa aatgaatacg cattagttca
     1201 ctaatttaag ttaagttatc cgacaactca ataacatcaa aaactgtgtt ttaacaataa
     1261 taaatgataa ttattttaaa attttctgaa ctggtcctat tattttggcg catgattttt
     1321 gggttatttt tacatttctt atcataactt ttttgtttct taacttttca ccaaacttta
     1381 aaaacattct taaagtaaac attctaatag tgctttcatg aaagtttggt aaagattgat
     1441 tcactttaag tcatgaaata tgataagtga ataataaatt gataagtggt cctattgatt
     1501 tgtccgacac tgtatacaat tgagagacgc aagacactgc ggatcgagac gcattgcgtg
     1561 gttcgactgg ccgtcgtttt acaacgaaag ggcctcgtga tacgcctatt tttataggtt
     1621 aatgtcatga taataatggt ttcttagacg tcaggtgctg atgaatcccc taatgatttt
     1681 tatcaaaatc attaagttaa ggtagataca catcttgtca tatgatcaaa tggtttcgcc
     1741 aaaaatcaat aatcagacaa caaaatgtgc gaactcgata ttttacacga ctctctttac
     1801 caattctgcc ccgaattaca cttaaaacga ctcaacagct taacgttggc ttgccacgcc
     1861 ttacttgact gtaaaactct cactcttacc gaacttggcc gtaacctgcc aaccaaagcg
     1921 agaacaaaac ataacatcaa acgaatcgac cgattgttag gtaatcgtca cctccacaaa
     1981 gagcgactcg ctgtataccg ttggcatgct agctttatct gttcgggcaa tacgatgccc
     2041 attgtacttg ttgactggtc tgatatccgt gagcaaaaac ggcttatggt attgcgagct
     2101 tcagtcgcac tacacggtcg ttctgttact ctttatgaga aagcgttccc gctttcagag
     2161 caatgttcaa agaaagctca tgaccaattt ctagccgacc ttgcgagcat tctaccgagt
     2221 aacaccacac cgctcattgt cagtgatgct ggctttaaag tgccatggta taaatccgtt
     2281 gagaagctgg gttggtactg gttaagtcga gtaagaggaa aagtacaata tgcagaccta
     2341 ggagcggaaa actggaaacc tatcagcaac ttacatgata tgtcatctag tcactcaaag
     2401 actttaggct ataagaggct gactaaaagc aatccaatct catgccaaat tctattgtat
     2461 aaatctcgct ctaaaggccg aaaaaatcag cgctcgacac ggactcattg tcaccacccg
     2521 tcacctaaaa tctactcagc gtcggcaaag gagccatgga ttctagcaac taacttacct
     2581 gttgaaattc gaacacccaa acaacttgtt aatatctatt cgaagcgaat gcagattgaa
     2641 gaaaccttcc gagacttgaa aagtcctgcc tacggactag gcctacgcca tagccgaacg
     2701 agcagctcag agcgttttga tatcatgctg ctaatcgccc tgatgcttca actaacatgt
     2761 tggcttgcgg gcgttcatgc tcagaaacaa ggttgggaca agcacttcca ggctaacaca
     2821 gtcagaaatc gaaacgtact ctcaacagtt cgcttaggca tggaagtttt gcggcattct
     2881 ggctacacaa taacaaggga agacttactc gtggctgcaa ccctactagc tcaaaattta
     2941 ttcacacatg gttacgcttt ggggaaatta tgaggggatc tctcagcgtc aggtggcact
     3001 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3061 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3121 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3181 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3241 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3301 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3361 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3421 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3481 tgtagtgctg ccataaccat gagtgataac acggcggcca acttacttct gacaacgatc
     3541 ggaggaccga aggagctaac cgcttttttg cgcaacatgg gggatcatgt aactcgcctt
     3601 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga taccacgatg
     3661 cctgtagcga tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3721 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3781 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtcc
     3841 cgcggtatta ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3901 acgacgggga gtcaggcaac tatggatgaa cgaaagagac agatcgctga gataggtgcc
     3961 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4021 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4081 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4141 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4201 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4261 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     4321 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4381 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4441 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4501 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4561 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4621 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4681 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4741 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4801 ttctttcctg cgttatcccc tgattctgtg ggtaaccgta ttaccgcctt tgagtgagct
     4861 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggca
     4921 cacaggaaac agctatgacc gtctcggttg gcagtgactc cgtctctggg ccctatacag
     4981 tgtcggacaa atcaatagga ccacttaata atgttttatt cacttatcac atttcattac
     5041 ctaaagtgaa tcaatattta ccaaattttc atgaaagcac ttttcggatg ttggcttaaa
     5101 gtctgctttg aaagtttgtt gataagttaa caaacaaaaa agttattata agaaatgttg
     5161 aaaaaacccc aaaaatcatg cgacaaaata ataggaccag tatagaaaat tttattattt
     5221 taaccattta tcattattgc ctataaataa ttacagctcc ggcttttaat tcgcaatgtg
     5281 gccagcccgt acatttgaaa aactaaagaa aataacaaaa cagccttatc ctgggctaac
     5341 ttggtagcat ctcgagggcc aggaagcttc cctttggtct ccccctctta agtaccacat
     5401 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     5461 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     5521 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5581 tgtccaaact caatcgatgc gagggggcag ggcctgcatg tgaagggcgt cgtaggtgtc
     5641 cttggtggct gtactgagac cctggtaaag gccatcgtgc cccttgcccc tccggcgctc
     5701 gcctttcatc ccaatctcac tgtaggcctc cgccatctta tctttctgca gttcattgta
     5761 caggccttcc tgagggttct tccttctcgg ctttcccccc atctcagggt cccggccacg
     5821 tctcttgtcc aaaacatcgt actcctctct tcgtcctaga ttgagctcgt tatagagctg
     5881 gttctggccc tgctggtacg cgggggcgtc tgcgctcctg ctgaacttca ctctggagcg
     5941 ataggctgcg aagtcgcgtg gtggggcata gggctggtaa tgcttgcggg tgggcccggg
     6001 gcggcgggga gtcatgttca tgtagtcact gtgcaggagc ctgctcctct tactcctcac
     6061 ccagaaaata ataaaggcca ctgttactag caagctatag caagccagga ctcccccaac
     6121 caccaccagc acccaaaaat cacaggcgaa gtccagcccc ctcgtgtgca ctgcgccccc
     6181 cgccgctggc cggcacgcct ctgggcgcag ggacaggggc tgcgacgcga tggtgggcgc
     6241 cggtgttggt ggtcgcggcg ctggcgtcgt ggtcagcact gtcagttgcg tgcctccacc
     6301 gaaaacagcg gctgaactat gccaaatcat acagtaataa tcggcctcgt cctcggacct
     6361 gagacctgag atgagcagca cacccgcatt ggcgcttgcg tctttagatc cactaaacct
     6421 tgatggaact ccagatccct gctgcttatc tgagtcgctc ttgtaattga gaaggtattg
     6481 gggaggagaa cctggttttt gctgatacca gtagatcctg taaggcccta cattgattcc
     6541 tgacctcagt gtacacgtca aagaagcgga ggcaccgggg cttgcactga gggaggatga
     6601 ttgcgtgaga acaggttggc tacccccccc tccggacccg cccccaccgg aacctccacc
     6661 gcctgagccc aggatcccac tggacacggt gacggttgta ccttgtcccc aaacgtccat
     6721 accatagtag taagtcatca taccccgcgc acaatagtaa acagctgtgt cttcgggagt
     6781 tacactatta agctgaagag agaactggtt tttggaagta tctggattta tagacatcct
     6841 gcttttcaca cttacagcat aatcattata ccatttactg cggtagtatg ttctaccgag
     6901 ccattccaac cctcgactgg gggactgcct gatccaattc catgtcgcgg agttactact
     6961 cactgaatca cctgatatgg cacaggtaag actaagagtc tgggaaggag tgaccagacc
     7021 ggggccagat tgttggagtt gcacttgcgg cctggcggcg tggagcagca aggccagcgg
     7081 caggagcaag gcggtcactg gtaaggcctt atcgtcatcg tctttgtaat ccatggtggc
     7141 gaccggtgga tccctcacga cacctgaaat ggaagaaaaa aactttgaac cactgtctga
     7201 ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa attccaagga gaattacatc
     7261 aagtgccaag ctggcctaac ttcagtctcc acccactcag tgtggggaaa ctccatcgca
     7321 taaaacccct ccccccaacc taaagacgac gtactccaaa agctcgagaa ctaatcgagg
     7381 tgcctggacg gcgcccggta ctcagtggag tcacatgaag cgacggctga ggacggaaag
     7441 gcccttttcc tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg cgtcctccat
     7501 tttgagctcc ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc aactggtgcc
     7561 gaccgggcca gccttgccgc ccagggcggg gcgatacacg gcggcgcgag gccaggcacc
     7621 agagcaggcc ggcgagcttg agactacccc cgtccgattc tcggtggccg cgctcgcagg
     7681 ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca
     7741 aaaaccgaaa taccagtgtg cagatcttgg cccgcattta caagactatc ttgccagaaa
     7801 aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta gagacttatc gaaagcagcg
     7861 agacaggcgc gaaggtgcca ccagattcgc acgcggcggc cccagcgccc aggccaggcc
     7921 tcaactcaag cacgaggcga aggggctcct taagcgcaag gcctcgaact ctcccaccca
     7981 cttccaaccc gaagctcggg atcaagaatc acgtactgca gccaggtgga agtaattcaa
     8041 ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc gggaaccaca cacggcactt
     8101 acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt
     8161 atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc
     8221 cagtttaccc cgcgccacct tctctaggca ccgggtcaat tgccgacccc tccccccaac
     8281 ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gcctcacgtt
     8341 aattaaccaa ggtacccatt ggcggccgcc ctcagaagcc atagagccca ccgcatcccc
     8401 agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc
     8461 cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag
     8521 gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga
     8581 tggctggcaa ctagaaggca cagtcgaggc tgatcagcga gcttaattaa ttacttgtac
     8641 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
     8701 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
     8761 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     8821 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
     8881 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
     8941 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
     9001 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
     9061 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
     9121 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
     9181 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
     9241 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
     9301 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat tggcccggga
     9361 ttctcttcga catcccctgc ttgtttcaac agggagaagt tagtggcatc gatggcaccg
     9421 ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg
     9481 gtgaagccga gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg
     9541 ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc
     9601 ttgccctggt ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc
     9661 cg
//
