LOCUS       sequence-446025-        7812 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mega' with knockout of GvpF and GvpK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaFK
FEATURES             Location/Qualifiers
     source          1..7812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6070..6088)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6084..6131
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6198..6289
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7154..7193
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7224..7812
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7713..7732
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaagggaa gagaaataac
     2701 gtgcttcaca aattagtaac cgcacccatt aaccttgtag tgaaaatcgg cgaaaaagta
     2761 caggaagaag ctgataaaca gctatatgac cttccgacga ttcagcaaaa gctcattcag
     2821 cttcaaatga tgtttgagct tggtgaaatt ccagaagaag cgtttcaaga aaaagaagat
     2881 gaattgttaa tgaggtacga aattgcgaaa cgcagagaaa ttgaacaatg ggaagagcta
     2941 acacaaaaaa gaaatgagga atcctagatg ggagaattac tgtatttata cggtttaatt
     3001 ccaacaaaag aagcagcagc catagagccg tttccatctt ataaggggtt tgacggagaa
     3061 cattcactgt acccaattgc gtttgatcag gtgacggctg tagtttctaa gctggatgct
     3121 gacacctatt cagaaaaagt gattcaagaa aaaatggagc aggatatgag ctggctgcag
     3181 gaaaaagcat ttcatcatca cgaaacggta gccgctttgt acgaagaatt tacgatcatt
     3241 ccattaaaat tttgcaccat ttataaaggt gaagaaagtc tgcaggcagc tattgagatt
     3301 aacaaagaaa agatagagaa ttcactgacg ctgcttcaag gaaatgaaga gtggaatgtg
     3361 aaaatttact gtgatgatac agagcttaaa aaaggaatca gcgaaacgaa tgaaagcgtg
     3421 aaagcgaaaa aacaagaaat tagtcactta tcaccaggaa gacagttttt tgaaaagaaa
     3481 aaaatagatc agctgattga aaaagaatta gagcttcaca aaaacaaagt gtgtgaagag
     3541 atacatgaca agctaaaaga attatcgctt tatgactctg ttaaaaagaa ttggagcaaa
     3601 gacgtaactg gcgcagctga acagatggcg tggaacagcg tgtttcttct cccgtctctg
     3661 cagattacta agttcgtaaa cgaaatagaa gagcttcagc aaaggcttga aaataaaggc
     3721 tggaagtttg aagtgacggg accatggccg ccctatcatt tctcgagctt tgcgtaaagt
     3781 gaggaattaa cattatgtct cttaaacaat ccatggagaa taaagatatt gctcttattg
     3841 atattttaga tgtcatttta gataaaggag tcgccattaa aggagactta atcatttcca
     3901 tagctggcgt cgatttagtg tatttggatt tgcgggtgct tatttcttcg gttgaaacgc
     3961 ttgtgcaagc aaaagaagga aatcacaaac caatcacttc tgaacaattt gataaacaaa
     4021 aagaagagct gatggacgct acgggccagc ctagtaagtg gacgaaccct ctaggttcgt
     4081 gacccttgcg agcagtcgta tgctttctcg aattccgagc ggttaagcgt gacaggcggt
     4141 cagtaggagg aacagtatgg cagtcgaaca taatatgcag tcaagtacga ttgtagatgt
     4201 gctcgaaaag attttggata aaggagtcgt tatagcgggg gacatcaccg taggaattgc
     4261 agatgtcgag ctattaacga taaagatccg cttgattgtg gcttcggttg ataaggcaaa
     4321 agaaatcggc atggactggt gggaaaatga tccgtatctc agttcaaaag gagccaataa
     4381 caaagcgctc gaagaagaaa ataaaatgct gcatgagcgg ttaaaaacgc ttgaagaaaa
     4441 aatagaaacg aaacgttaaa aactgtacgc tacttaaaaa atggagggat ttacaatggc
     4501 aactgaaaca aaattagata acacacaggc agaaaacaag gaaaataaaa atgcggaaaa
     4561 cggttcaaaa gaaaagaacg gttcaaaagc aagcaaaaca acaagcagcg ggccaatcaa
     4621 acgagcggta gcaggaggca tcatcggtgc aacgattgga tatgtatcga ctcctgaaaa
     4681 tcgaaaaagt ctccttgacc gcattgatac agacgaatta aaaagcaaag catctgattt
     4741 aggaacaaag gtaaaagaaa aatcaaaaag cagcgtggcc agcctgaaaa catctgcggg
     4801 aagcttgttt aaaaaagata aagataaatc aaaagatgat gaagaaaacg taaattcttc
     4861 tagtagcgaa acagaagacg ataacgttca agagtacgac gagttaaaag aagaaaatca
     4921 aactcttcaa gatcgcttat cacagcttga agaaaaaatg aacatgcttg ttgagcttag
     4981 cctcaataaa aatcaagacg aagaagcgga agatacagat tccgacgaag aagagaacga
     5041 tgagaacgat gaaaacgatg aaaacgagca ggacgatgaa aacgaagaag aaacatctaa
     5101 gccacgtaaa aaggataaaa aagaagctga ggaagaagaa agtgaaagtg acgaagacag
     5161 cgaggaagaa gaggaagatt ctcgctcaaa caaaaaaaat aaaaaagtaa aaacagaaga
     5221 agaagacgaa gatgaaagcg aagaagaaaa aaaggaagcg aaaccaaaaa agtcaacagc
     5281 taaaaaatca aaaaatacaa aagcaaagaa aaacacggac gaagaagatg atgaagcaac
     5341 atctctttct agtgaagacg atacaacagc ctaagacgta aaggaggaaa gaaagacatg
     5401 agtacaggcc cttctttttc aactaaagac aatacgcttg aatactttgt gaaagcttct
     5461 aataaacacg gcttttcact tgatatttca ttaaatgtaa acggcgctgt gatttccggt
     5521 accatgattt cagcaaaaga atactttgat tacttaagcg aaacgtttga agaaggcagt
     5581 gaagtggctc aggcgctaag cgaacaattc tctttagcaa gcgaagcgag cgaatcaaac
     5641 ggagaagcag aagcccattt tattcatttg aaaaatacaa agatttactg tggagacagt
     5701 aaatctactc cttctaaagg caaaatcttt tggagaggga aaatagcaga agtagacggg
     5761 tttttcttag gaaagatttc tgatgcaaaa tcaacgagta aaaagagttc ataaaaaacg
     5821 gcggggtgat tgccccgccg ttttttagtg atgtgatgag atgtgcagct tcttttttcg
     5881 atacatgagc ggagtcatct tcatttcttt tcgaaactga ttaataaaat agcttgtact
     5941 attaaaccct acttgatagg cgacttccgt cacatttgct tctgcttgct gcagtccgga
     6001 ttcgcgaacg cgtctcgaga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     6061 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     6121 ggggtttttt gctgaaagga ggaactatat ccgactagta gcccgcctaa tgagcgggct
     6181 tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6241 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6301 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6361 cccagaaacg ctcgtgaaag taaaagacgc agaggaccaa ttgggggcac gagtgggata
     6421 catagaactg gacttgaata gcggtaaaat ccttgagagt tttcgccctg aagagcgttt
     6481 tccaatgatg agcactttca aagttctgct atgtggagca gtattatccc gtgtagatgc
     6541 ggggcaagag caactcggac gacgaataca ctattcgcag aatgacttgg ttgaatactc
     6601 cccagtgaca gaaaagcacc ttacggacgg aatgacggta agagaattat gtagtgccgc
     6661 cataacgatg agtgataaca ctgcggcgaa cttacttctg acaaccatcg gtggaccgaa
     6721 ggaattaacc gcttttttgc acaatatggg agaccatgta actcgccttg accgttggga
     6781 accagaactg aatgaagcca taccaaacga cgagcgagac accacaatgc ctgcggcaat
     6841 ggcaacaaca ttacgcaaac tattaactgg cgaactactt actctggctt cacggcaaca
     6901 attaatagac tggcttgaag cggataaagt tgcaggacca ctactgcgtt cggcacttcc
     6961 tgctggctgg tttattgctg ataaatctgg ggcaggagag cgtggttcac ggggtatcat
     7021 tgccgcactt ggaccagatg gtaagccttc ccgtatcgta gttatctaca cgacgggtag
     7081 tcaggcaact atggacgaac gaaatagaca gattgctgaa ataggggctt cactgattaa
     7141 gcattggtaa accgatacaa ttaaaggctc cttttggagc cttttttttt ggacggaccg
     7201 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7261 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7321 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     7381 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7441 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7501 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7561 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7621 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7681 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7741 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7801 agcctatgga aa
//
