LOCUS       sequence-446208-        8424 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway entry clone (attL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Contiliani DF, Sretenovic S, Dailey M, Zhou M, Cheng Y, Creste S, 
            Xiao S, Qi Y
  TITLE     Harnessing novel cytidine deaminases from the animal kingdom for 
            robust multiplexed base editing in rice.
  JOURNAL   Plant Biotechnol J. 2025 Feb 14. doi: 10.1111/pbi.70000.
  PUBMED    39950393
REFERENCE   2  (bases 1 to 8424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2025 Feb 14. doi: 10.1111/pbi.70000."
COMMENT     Sequence Label: pYPQ265_LoA3GX1
FEATURES             Location/Qualifiers
     source          1..8424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2160..2187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2279..2365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3284..3349
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3356..3376
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3401..3424,3425..3427,3428..7501)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7414..7422
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7502..7549
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7571..7819
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7832..7852
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7874..8126
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8171..8294
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8311..8330)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8312..8330)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8335..8351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8335..8351)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcatgtg
     2581 gggagctgag ggcaaatctg tcagaggagg agaagagagg tgggcagttc ctgaaggagc
     2641 accatgtctt tgggcatctc cagcctttga cagatcctgc ctgctcgatg aaaacacatt
     2701 cactgagaat tttatgaacc aaatgtcccc acgcaaaaca tacctctgtt acaaggtgga
     2761 gatacttgac ggagatgcta gaataccact cgacgaaaag aagggcttcg tccggaataa
     2821 aggtgctaat gagcccggcg gaccgcgcca tgctgaaagt tactttctcg accgcatcag
     2881 atcttggaat ttggaccggg acctccatta ccgcctgacc tgttttattt catggacgcc
     2941 ttgtgatact tgtgcgcaag aactggctga ttttctcgga aaaaactccc atgtgtccct
     3001 gcatattttc gcatctagga tttactctcg ctttggatat gaggacggat tgagaacact
     3061 tcaggcagct ggcgctcaaa ttgccattat ggcttctaat gagtttgagc attgctggga
     3121 gaactttgtt gaccaccagg gaaagccctt tcagccctgg gacggcttgg aagtggtgtc
     3181 tcaacgcctt tgcaataacc ttcaggcaat attgcaaacg caggagaact caggttctga
     3241 gacccctggc acaagtgagt cagcaacacc cgagtccacc atggattaca aggaccacga
     3301 cggggattac aaggaccacg acattgatta caaggatgat gatgacaaga tggctccgaa
     3361 gaagaagagg aaggttggca tccacggggt gccagctgct gacaagaagt actcgatcgg
     3421 cctcgctatt gggactaact ctgttggctg ggccgtgatc accgacgagt acaaggtgcc
     3481 ctcaaagaag ttcaaggtcc tgggcaacac cgatcggcat tccatcaaga agaatctcat
     3541 tggcgctctc ctgttcgaca gcggcgagac ggctgaggct acgcggctca agcgcaccgc
     3601 ccgcaggcgg tacacgcgca ggaagaatcg catctgctac ctgcaggaga ttttctccaa
     3661 cgagatggcg aaggttgacg attctttctt ccacaggctg gaggagtcat tcctcgtgga
     3721 ggaggataag aagcacgagc ggcatccaat cttcggcaac attgtcgacg aggttgccta
     3781 ccacgagaag taccctacga tctaccatct gcggaagaag ctcgtggact ccacagataa
     3841 ggcggacctc cgcctgatct acctcgctct ggcccacatg attaagttca ggggccattt
     3901 cctgatcgag ggggatctca acccggacaa tagcgatgtt gacaagctgt tcatccagct
     3961 cgtgcagacg tacaaccagc tcttcgagga gaaccccatt aatgcgtcag gcgtcgacgc
     4021 gaaggctatc ctgtccgcta ggctctcgaa gtctcggcgc ctcgagaacc tgatcgccca
     4081 gctgccgggc gagaagaaga acggcctgtt cgggaatctc attgcgctca gcctggggct
     4141 cacgcccaac ttcaagtcga atttcgatct cgctgaggac gccaagctgc agctctccaa
     4201 ggacacatac gacgatgacc tggataacct cctggcccag atcggcgatc agtacgcgga
     4261 cctgttcctc gctgccaaga atctgtcgga cgccatcctc ctgtctgata ttctcagggt
     4321 gaacaccgag attacgaagg ctccgctctc agcctccatg atcaagcgct acgacgagca
     4381 ccatcaggat ctgaccctcc tgaaggcgct ggtcaggcag cagctccccg agaagtacaa
     4441 ggagatcttc ttcgatcagt cgaagaacgg ctacgctggg tacattgacg gcggggcctc
     4501 tcaggaggag ttctacaagt tcatcaagcc gattctggag aagatggacg gcacggagga
     4561 gctgctggtg aagctcaatc gcgaggacct cctgaggaag cagcggacat tcgataacgg
     4621 cagcatccca caccagattc atctcgggga gctgcacgct atcctgagga ggcaggagga
     4681 cttctaccct ttcctcaagg ataaccgcga gaagatcgag aagattctga ctttcaggat
     4741 cccgtactac gtcggcccac tcgctagggg caactcccgc ttcgcttgga tgacccgcaa
     4801 gtcagaggag acgatcacgc cgtggaactt cgaggaggtg gtcgacaagg gcgctagcgc
     4861 tcagtcgttc atcgagagga tgacgaattt cgacaagaac ctgccaaatg agaaggtgct
     4921 ccctaagcac tcgctcctgt acgagtactt cacagtctac aacgagctga ctaaggtgaa
     4981 gtatgtgacc gagggcatga ggaagccggc tttcctgtct ggggagcaga agaaggccat
     5041 cgtggacctc ctgttcaaga ccaaccggaa ggtcacggtt aagcagctca aggaggacta
     5101 cttcaagaag attgagtgct tcgattcggt cgagatctct ggcgttgagg accgcttcaa
     5161 cgcctccctg gggacctacc acgatctcct gaagatcatt aaggataagg acttcctgga
     5221 caacgaggag aatgaggata tcctcgagga cattgtgctg acactcactc tgttcgagga
     5281 ccgggagatg atcgaggagc gcctgaagac ttacgcccat ctcttcgatg acaaggtcat
     5341 gaagcagctc aagaggagga ggtacaccgg ctgggggagg ctgagcagga agctcatcaa
     5401 cggcattcgg gacaagcagt ccgggaagac gatcctcgac ttcctgaaga gcgatggctt
     5461 cgcgaaccgc aatttcatgc agctgattca cgatgacagc ctcacattca aggaggatat
     5521 ccagaaggct caggtgagcg gccaggggga ctcgctgcac gagcatatcg cgaacctcgc
     5581 tggctcgcca gctatcaaga aggggattct gcagaccgtg aaggttgtgg acgagctggt
     5641 gaaggtcatg ggcaggcaca agcctgagaa catcgtcatt gagatggccc gggagaatca
     5701 gaccacgcag aagggccaga agaactcacg cgagaggatg aagaggatcg aggagggcat
     5761 taaggagctg gggtcccaga tcctcaagga gcacccggtg gagaacacgc agctgcagaa
     5821 tgagaagctc tacctgtact acctccagaa tggccgcgat atgtatgtgg accaggagct
     5881 ggatattaac aggctcagcg attacgacgt cgatcatatc gttccacagt cattcctgaa
     5941 ggatgactcc attgacaaca aggtcctcac caggtcggac aagaaccggg gcaagtctga
     6001 taatgttcct tcagaggagg tcgttaagaa gatgaagaac tactggcgcc agctcctgaa
     6061 tgccaagctg atcacgcagc ggaagttcga taacctcaca aaggctgaga ggggcgggct
     6121 ctctgagctg gacaaggcgg gcttcatcaa gaggcagctg gtcgagacac ggcagatcac
     6181 taagcacgtt gcgcagattc tcgactcacg gatgaacact aagtacgatg agaatgacaa
     6241 gctgatccgc gaggtgaagg tcatcaccct gaagtcaaag ctcgtctccg acttcaggaa
     6301 ggatttccag ttctacaagg ttcgggagat caacaattac caccatgccc atgacgcgta
     6361 cctgaacgcg gtggtcggca cagctctgat caagaagtac ccaaagctcg agagcgagtt
     6421 cgtgtacggg gactacaagg tttacgatgt gaggaagatg atcgccaagt cggagcagga
     6481 gattggcaag gctaccgcca agtacttctt ctactctaac attatgaatt tcttcaagac
     6541 agagatcact ctggccaatg gcgagatccg gaagcgcccc ctcatcgaga cgaacggcga
     6601 gacgggggag atcgtgtggg acaagggcag ggatttcgcg accgtcagga aggttctctc
     6661 catgccacaa gtgaatatcg tcaagaagac agaggtccag actggcgggt tctctaagga
     6721 gtcaattctg cctaagcgga acagcgacaa gctcatcgcc cgcaagaagg actgggatcc
     6781 gaagaagtac ggcgggttcg acagccccac tgtggcctac tcggtcctgg ttgtggcgaa
     6841 ggttgagaag ggcaagtcca agaagctcaa gagcgtgaag gagctgctgg ggatcacgat
     6901 tatggagcgc tccagcttcg agaagaaccc gatcgatttc ctggaggcga agggctacaa
     6961 ggaggtgaag aaggacctga tcattaagct ccccaagtac tcactcttcg agctggagaa
     7021 cggcaggaag cggatgctgg cttccgctgg cgagctgcag aaggggaacg agctggctct
     7081 gccgtccaag tatgtgaact tcctctacct ggcctcccac tacgagaagc tcaagggcag
     7141 ccccgaggac aacgagcaga agcagctgtt cgtcgagcag cacaagcatt acctcgacga
     7201 gatcattgag cagatttccg agttctccaa gcgcgtgatc ctggccgacg cgaatctgga
     7261 taaggtcctc tccgcgtaca acaagcaccg cgacaagcca atcagggagc aggctgagaa
     7321 tatcattcat ctcttcaccc tgacgaacct cggcgcccct gctgctttca agtacttcga
     7381 cacaactatc gatcgcaaga ggtacacaag cactaaggag gtcctggacg cgaccctcat
     7441 ccaccagtcg attaccggcc tctacgagac gcgcatcgac ctgtctcagc tcgggggcga
     7501 caagcggcca gcggcgacga agaaggcggg gcaggcgaag aagaagaagg gagacggatc
     7561 aggcgggtca acaaacctca gtgacatcat agagaaggaa actggtaagc aactggttat
     7621 tcaagagagc attctgatgc tccctgagga agtcgaggaa gttataggaa acaagcctga
     7681 gagcgatata ctcgtgcata cggcgtatga cgagagcacg gatgaaaatg tgatgctctt
     7741 gaccagcgat gcgccagaat ataaaccctg ggcactggtt attcaggact ctaacgggga
     7801 aaataaaatc aagatgctct caggtggctc cccaaagaag aaacgcaagg tttagtggcg
     7861 gccgccgacg tccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     7921 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     7981 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     8041 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     8101 cgcggtgtca tctatgttac tagatcggga attgatcccc cctcgacagc ttccggaaag
     8161 ggcgaattcg caactttgta tacaaaagtt gaacgagaaa cgtaaaatga tataaatatc
     8221 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     8281 atccagtcac tatgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     8341 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     8401 aaaaatatat catcatgcct cctc
//
