LOCUS       sequence-446220-        6035 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Localization: Gap Junctions, Excitation: 574, Emission: 596.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6035)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mStrawberry-Cx43-7
FEATURES             Location/Qualifiers
     source          1..6035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1931..2641
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2076..2094)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2344..2363
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2763..2884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2800..2819)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2854..2873
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2891..3346)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3028..3049)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3240..3259
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3373..3477
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3479..3836
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3479..3670
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3502..3522)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3687..3822
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3871..4665
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3871..4665
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3925..3944)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4535..4554
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4853..4872)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4897..4944
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5273..5861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5762..5781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttcga attcggcagc acttttcttt cattgggggg
      601 aaggcgtgag gaaagtacca aacagcagca gacttttaaa ctttaaacag acaggtctga
      661 gagcctgaac tctcattttt cctttgactt cagcctccaa ggagttccac caactttggc
      721 gcgccggctt cactttcatt aagtgaaaga gaggtgccca gacatgggtg actggagtgc
      781 cttggggaag cttctggaca aggtccaagc ctactccacc gctggaggga aggtgtggct
      841 gtcagtgctc ttcatattca gaatcctgct cctggggaca gctgttgagt cagcttgggg
      901 tgatgaacag tctgcctttc gctgtaacac tcaacaacct ggctgcgaaa acgtctgcta
      961 tgacaagtcc ttccccatct ctcacgtgcg cttctgggtc cttcagatca tattcgtgtc
     1021 tgtgcccaca ctcctgtact tggcccatgt gttctatgtg atgaggaagg aagagaagct
     1081 aaacaagaaa gaagaggagc tcaaagtggc ccagactgac ggggtcaacg tggagatgca
     1141 cctgaagcag attgaaatca agaagttcaa gtacgggatt gaagagcacg gcaaggtgaa
     1201 aatgaggggc ggcttgctga gaacctacat catcagcatc ctcttcaagt ctgtcttcga
     1261 ggtggccttc ctgctcatcc agtggtacat ctatgggttc agcttgagcg cggtctacac
     1321 ctgcaagaga gatccctgcc cccaccaggt agactgcttc ctctcacgtc ccacggagaa
     1381 aaccatcttc atcatcttca tgctggtggt gtccttggtg tctctcgctt tgaacatcat
     1441 tgagctcttc tacgtcttct tcaagggcgt taaggatcgc gtgaagggaa gaagcgatcc
     1501 ttaccacgcc accactggcc cactgagccc atcaaaagac tgcggatctc caaaatacgc
     1561 ctacttcaat ggctgctcct caccaacggc tccactctcg cctatgtctc ctcctgggta
     1621 caagctggtt actggtgaca gaaacaattc ctcgtgccgc aattacaaca agcaagctag
     1681 cgagcaaaac tgggcgaact acagcgcaga gcaaaatcgc atggggcagg ccggaagcac
     1741 catctccaac tcgcacgccc agccgttcga tttccccgac gacaaccaga atgccaaaaa
     1801 agttgctgct ggacatgaac tccagccatt agccatcgtg gaccaacgac cttccagcag
     1861 agccagcagc cgcgccagca gcaggcctcg gcctgatgac ctggagattg cggatccacc
     1921 ggtggctacc atggtgagca agggcgagga gaataacatg gccatcatca aggagttcat
     1981 gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     2041 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     2101 ccccctgccc ttcgcctggg acatcctaac ccccaacttc acctacggct ccaaggccta
     2161 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     2221 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     2281 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     2341 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     2401 cgaggacggc gccctgaagg gcgagatcaa gatgaggctg aagctgaagg acggcggcca
     2461 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     2521 ctacatcgtc ggcatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     2581 actgtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     2641 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     2701 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     2761 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2821 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2881 cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     2941 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     3001 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     3061 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     3121 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     3181 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3241 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     3301 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     3361 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3421 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
     3481 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     3541 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     3601 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3661 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3721 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3781 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     3841 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     3901 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     3961 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     4021 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     4081 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     4141 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     4201 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     4261 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     4321 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     4381 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     4441 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     4501 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     4561 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     4621 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     4681 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     4741 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     4801 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
     4861 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     4921 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     4981 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     5041 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     5101 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
     5161 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5221 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5281 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5341 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5401 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5461 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5521 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5581 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5641 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5701 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5761 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5821 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5881 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5941 ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg
     6001 gggtcattag ttcatagccc atatatggag ttccg
//
