LOCUS       sequence-446791-       14360 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector plasmid expressing human reticulon 4 (RTN4) under 
            the spleen focus-forming virus (SFFV) promoter and Emerald under the
            Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14360)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-RTN4-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..14360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2414..2423
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6112..6132)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6530..6541)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7580..7599
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      7773..7782
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7779..7781,7782..7784,7785..8498)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7824..7845)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8085..8104)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8432..8453
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             8593..8826
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8851..8869)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8852..8869)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9159..9178)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9392..9411)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9511..9533
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9571..9589)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9657..9761
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9762..9830,9831..10622)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9980..9999)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10793..11381
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11282..11301
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11535..11552
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11622..11642)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11627..11956
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11807..11942
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          13090..13155
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13285..13305
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13730..13864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13767..13786)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13821..13840
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(14032..14360,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccaccg gtcgccacca tggaagacct ggaccagtct cctctggtct cgtcctcgga
     2461 cagcccaccc cggccgcagc ccgcgttcaa gtaccagttc gtgagggagc ccgaggacga
     2521 ggaggaagaa gaggaggagg aagaggagga cgaggacgaa gacctggagg agctggaggt
     2581 gctggagagg aagcccgccg ccgggctgtc cgcggcccca gtgcccaccg cccctgccgc
     2641 cggcgcgccc ctgatggact tcggaaatga cttcgtgccg ccggcgcccc ggggacccct
     2701 gccggccgct ccccccgtcg ccccggagcg gcagccgtct tgggacccga gcccggtgtc
     2761 gtcgaccgtg cccgcgccat ccccgctgtc tgctgccgca gtctcgccct ccaagctccc
     2821 tgaggacgac gagcctccgg cccggcctcc ccctcctccc ccggccagcg tgagccccca
     2881 ggcagagccc gtgtggaccc cgccagcccc ggctcccgcc gcgcccccct ccaccccggc
     2941 cgcgcccaag cgcaggggct cctcgggctc agtggatgag accctttttg ctcttcctgc
     3001 tgcatctgag cctgtgatac gctcctctgc agaaaatatg gacttgaagg agcagccagg
     3061 taacactatt tcggctggtc aagaggattt cccatctgtc ctgcttgaaa ctgctgcttc
     3121 tcttccttct ctgtctcctc tctcagccgc ttctttcaaa gaacatgaat accttggtaa
     3181 tttgtcaaca gtattaccca ctgaaggaac acttcaagaa aatgtcagtg aagcttctaa
     3241 agaggtctca gagaaggcaa aaactctact catagataga gatttaacag agttttcaga
     3301 attagaatac tcagaaatgg gatcatcgtt cagtgtctct ccaaaagcag aatctgccgt
     3361 aatagtagca aatcctaggg aagaaataat cgtgaaaaat aaagatgaag aagagaagtt
     3421 agttagtaat aacatccttc ataatcaaca agagttacct acagctctta ctaaattggt
     3481 taaagaggat gaagttgtgt cttcagaaaa agcaaaagac agttttaatg aaaagagagt
     3541 tgcagtggaa gctcctatga gggaggaata tgcagacttc aaaccatttg agcgagtatg
     3601 ggaagtgaaa gatagtaagg aagatagtga tatgttggct gctggaggta aaatcgagag
     3661 caacttggaa agtaaagtgg ataaaaaatg ttttgcagat agccttgagc aaactaatca
     3721 cgaaaaagat agtgagagta gtaatgatga tacttctttc cccagtacgc cagaaggtat
     3781 aaaggatcgt tcaggagcat atatcacatg tgctcccttt aacccagcag caactgagag
     3841 cattgcaaca aacatttttc ctttgttagg agatcctact tcagaaaata agaccgatga
     3901 aaaaaaaata gaagaaaaga aggcccaaat agtaacagag aagaatacta gcaccaaaac
     3961 atcaaaccct tttcttgtag cagcacagga ttctgagaca gattatgtca caacagataa
     4021 tttaacaaag gtgactgagg aagtcgtggc aaacatgcct gaaggcctga ctccagattt
     4081 agtacaggaa gcatgtgaaa gtgaattgaa tgaagttact ggtacaaaga ttgcttatga
     4141 aacaaaaatg gacttggttc aaacatcaga agttatgcaa gagtcactct atcctgcagc
     4201 acagctttgc ccatcatttg aagagtcaga agctactcct tcaccagttt tgcctgacat
     4261 tgttatggaa gcaccattga attctgcagt tcctagtgct ggtgcttccg tgatacagcc
     4321 cagctcatca ccattagaag cttcttcagt taattatgaa agcataaaac atgagcctga
     4381 aaacccccca ccatatgaag aggccatgag tgtatcacta aaaaaagtat caggaataaa
     4441 ggaagaaatt aaagagcctg aaaatattaa tgcagctctt caagaaacag aagctcctta
     4501 tatatctatt gcatgtgatt taattaaaga aacaaagctt tctgctgaac cagctccgga
     4561 tttctctgat tattcagaaa tggcaaaagt tgaacagcca gtgcctgatc attctgagct
     4621 agttgaagat tcctcacctg attctgaacc agttgactta tttagtgatg attcaatacc
     4681 tgacgttcca caaaaacaag atgaaactgt gatgcttgtg aaagaaagtc tcactgagac
     4741 ttcatttgag tcaatgatag aatatgaaaa taaggaaaaa ctcagtgctt tgccacctga
     4801 gggaggaaag ccatatttgg aatcttttaa gctcagttta gataacacaa aagataccct
     4861 gttacctgat gaagtttcaa cattgagcaa aaaggagaaa attcctttgc agatggagga
     4921 gctcagtact gcagtttatt caaatgatga cttatttatt tctaaggaag cacagataag
     4981 agaaactgaa acgttttcag attcatctcc aattgaaatt atagatgagt tccctacatt
     5041 gatcagttct aaaactgatt cattttctaa attagccagg gaatatactg acctagaagt
     5101 atcccacaaa agtgaaattg ctaatgcccc ggatggagct gggtcattgc cttgcacaga
     5161 attgccccat gacctttctt tgaagaacat acaacccaaa gttgaagaga aaatcagttt
     5221 ctcagatgac ttttctaaaa atgggtctgc tacatcaaag gtgctcttat tgcctccaga
     5281 tgtttctgct ttggccactc aagcagagat agagagcata gttaaaccca aagttcttgt
     5341 gaaagaagct gagaaaaaac ttccttccga tacagaaaaa gaggacagat caccatctgc
     5401 tatattttca gcagagctga gtaaaacttc agttgttgac ctcctgtact ggagagacat
     5461 taagaagact ggagtggtgt ttggtgccag cctattcctg ctgctttcat tgacagtatt
     5521 cagcattgtg agcgtaacag cctacattgc cttggccctg ctctctgtga ccatcagctt
     5581 taggatatac aagggtgtga tccaagctat ccagaaatca gatgaaggcc acccattcag
     5641 ggcatatctg gaatctgaag ttgctatatc tgaggagttg gttcagaagt acagtaattc
     5701 tgctcttggt catgtgaact gcacgataaa ggaactcagg cgcctcttct tagttgatga
     5761 tttagttgat tctctgaagt ttgcagtgtt gatgtgggta tttacctatg ttggtgcctt
     5821 gtttaatggt ctgacactac tgattttggc tctcatttca ctcttcagtg ttcctgttat
     5881 ttatgaacgg catcaggcac agatagatca ttatctagga cttgcaaata agaatgttaa
     5941 agatgctatg gctaaaatcc aagcaaaaat ccctggattg aagcgcaaag ctgaatgaag
     6001 cggccgcgac tctagagtcg acctgcaggc atgcaagctt gatatcaagc ttatcgataa
     6061 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6121 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6181 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6241 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6301 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6361 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6421 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6481 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6541 tccagcggac cttccttccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag
     6601 acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct
     6661 gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact
     6721 tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt
     6781 cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata
     6841 aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct
     6901 tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc
     6961 tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg
     7021 tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat
     7081 ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga
     7141 aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc
     7201 gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag
     7261 tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt
     7321 gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca
     7381 cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct
     7441 tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg
     7501 ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt
     7561 acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt
     7621 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
     7681 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
     7741 gacgaagctt ggtaccgagc tcggatcaat tcgccaccat ggtgagcaag ggcgaggagc
     7801 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     7861 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     7921 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttgacctacg
     7981 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     8041 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     8101 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     8161 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     8221 gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     8281 cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     8341 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     8401 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     8461 ccgggatcac tctcggcatg gacgagctgt acaagtaact cgagggaatt aattcgagct
     8521 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     8581 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     8641 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8701 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8761 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8821 ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat
     8881 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa
     8941 ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag
     9001 agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat
     9061 ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt
     9121 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     9181 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     9241 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     9301 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     9361 taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     9421 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     9481 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     9541 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     9601 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     9661 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9721 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     9781 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     9841 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9901 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9961 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    10021 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    10081 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    10141 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    10201 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    10261 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    10321 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    10381 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    10441 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    10501 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    10561 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    10621 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    10681 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    10741 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    10801 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    10861 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10921 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10981 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    11041 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    11101 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    11161 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    11221 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    11281 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    11341 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    11401 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    11461 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    11521 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    11581 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
    11641 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    11701 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    11761 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
    11821 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    11881 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    11941 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
    12001 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
    12061 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
    12121 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
    12181 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
    12241 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
    12301 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
    12361 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
    12421 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
    12481 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
    12541 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
    12601 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
    12661 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
    12721 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
    12781 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
    12841 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
    12901 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
    12961 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
    13021 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
    13081 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
    13141 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
    13201 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
    13261 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
    13321 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
    13381 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
    13441 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
    13501 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
    13561 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
    13621 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
    13681 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    13741 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    13801 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    13861 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    13921 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    13981 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
    14041 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
    14101 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
    14161 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
    14221 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
    14281 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
    14341 cggagtactt caagaactgc
//
