LOCUS       sequence-446793-       15047 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector plasmid expressing human ribosome binding protein 
            1 (RRBP1) under the spleen focus-forming virus (SFFV) promoter and 
            Emerald under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15047)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-myc-RRBP1-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..15047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2414..2423
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2423..2452
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(6799..6819)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7217..7228)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8267..8286
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      8460..8469
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8466..8468,8469..8471,8472..9185)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8511..8532)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8772..8791)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9119..9140
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             9280..9513
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9538..9556)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9539..9556)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9846..9865)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10079..10098)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10198..10220
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10258..10276)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10344..10448
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10449..10517,10518..11309)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10667..10686)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11480..12068
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11969..11988
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12222..12239
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12309..12329)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12314..12643
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12494..12629
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          13777..13842
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13972..13992
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    14417..14551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14454..14473)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14508..14527
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(14719..15047,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccaccg gtcgccacca tggaacaaaa actcatctca gaagaggatc tgatggatat
     2461 ttacgacact caaaccttgg gggttgtggt ctttggagga ttcatggttg tttctgccat
     2521 tggcatcttc ctggtgtcga ctttctccat gaaggaaacg tcatatgaag aagccctagc
     2581 caaccagcgc aaggagatgg cgaaaactca ccaccagaaa gtcgagaaga aaaagaagga
     2641 gaaaacagtg gagaagaaag gaaagaccaa gaaaaaggaa gagaaaccta atgggaagat
     2701 acctgatcat gatccagccc ccaatgtgac tgtcctcctt cgagaaccag tgcgggctcc
     2761 tgctgtggct gtggctccaa ccccagtgca gccccccatt atcgttgctc ctgtcgccac
     2821 agttccagcc atgccccagg agaagctggc ctcctccccc aaggacaaaa agaagaagga
     2881 gaaaaaagtg gcaaaagtgg aaccagctgt cagctctgta gtgaattcca tccaggttct
     2941 cacttcgaag gctgccatct tggaaactgc tcccaaggag gtgccgatgg tggtggtgcc
     3001 cccagtgggt gccaagggca acacaccagc cactggcact actcagggca aaaaggcgga
     3061 ggggactcag aatcaaagca aaaaggctga aggagcccca aaccagggca gaaaggcaga
     3121 gggaacccca aaccagggca aaaagacaga gggaacccca aaccaaggga aaaaggcaga
     3181 gggaacccca aaccaaggca aaaaggcaga aggaacccca aaccaaggca aaaaggcgga
     3241 gggggcccag aaccagggta aaaaggtaga tacaacccca aaccagggga aaaaggtgga
     3301 gggggcccca acccagggca gaaaggccga gggggctcag aaccaggcca aaaaggtaga
     3361 aggggcccag aaccagggca aaaaggcaga gggggcccag aatcagggca aaaagggaga
     3421 gggggcccag aaccagggca agaaggccga gggggcccag aatcagggca agaaggccga
     3481 gggggcccag aatcagggca agaaggccga gggggcccag aatcagggca agaaggccga
     3541 gggggcccag aatcagggca agaaggctga gggggctcag aaccagggca aaaaggccga
     3601 gggggctcag aaccagggca aaaaagtaga aggggcccag aaccagggca agaaggctga
     3661 gggtgcccag aaccagggca aaaaggccga gggggcccag aatcagggca aaaaggccga
     3721 gggggcccag aaccagggca agaaggccga gggggcccag aaccagggca agaaggccga
     3781 gggggcccag aaccagggca agaaggccga gggggcccag aaccagggca agaaggccga
     3841 gggtgctcag aaccagggca aaaaagtaga aggggcccag aaccagggca agaaggctga
     3901 gggggcccag aaccagggca agaaggccga gggggctcag aaccagggca aaaaggccga
     3961 gggagcccag aaccagggcc aaaaaggaga gggagcccag aatcagggta aaaagacaga
     4021 aggggctcag ggcaaaaagg cagaaaggag tcccaaccaa ggcaaaaaag gagagggagc
     4081 tcccatccag ggcaaaaagg cagattcggt tgctaatcag ggcacaaagg tagagggtat
     4141 tacaaaccag gggaaaaaag cagaagggtc ccccagtgaa ggcaaaaagg cagaagggtc
     4201 ccccaaccaa ggcaaaaagg cagacgcagc tgccaatcag ggtaaaaaga cagagtcagc
     4261 ttctgtccag ggcagaaata cagatgtggc ccagagccca gaggcaccaa agcaagaggc
     4321 tcctgccaag aagaagtctg gttcaaagaa aaaaggtgag cctgggcccc cagatgccga
     4381 cggccctctc tacctcccct acaagacgct ggtctccacg gttgggagca tggtgttcaa
     4441 cgagggcgag gcccagcggc tcatcgagat cctgtctgag aaggctggca tcattcagga
     4501 cacctggcac aaggccactc agaagggtga ccctgtggcg attctgaaac gccagctgga
     4561 agagaaggaa aaactgctgg ccacagaaca ggaagatgcg gctgtcgcca agagcaaact
     4621 gagggagctc aacaaggaga tggcagcaga aaaggccaaa gcagcagccg gggaggccaa
     4681 agtgaaaaag cagctggtgg cccgggagca ggagatcacg gctgtgcagg cacgcatgca
     4741 ggccagctac cgggagcacg tgaaggaggt gcagcagctg cagggcaaga tccggactct
     4801 tcaggagcag ctggagaatg gccccaacac gcagctggcc cgcctgcagc aggagaactc
     4861 catcctgcgg gatgccttga accaggccac gagccaggtg gagagcaagc agaacgcaga
     4921 gctggccaag cttcggcagg agctcagcaa ggtcagcaaa gagctggtgg agaagtcaga
     4981 ggctgtgcgg caagatgagc agcagcggaa agctctggaa gccaaggcag ctgccttcga
     5041 gaagcaggtc ctgcagctgc aggcgtccca cagggagagt gaggaggccc tgcagaagcg
     5101 cctggacgag gtcagccggg agctgtgcca cacgcagagc agccacgcca gcctccgggc
     5161 ggatgccgag aaggcccagg agcaacagca gcagatggcc gagctgcaca gcaagttaca
     5221 gtcctccgag gcggaggtgc gcagcaaatg cgaggagctg agtggcctcc acgggcagct
     5281 ccaggaggcc agggcggaga actcccagct cacagagaga atccgttcca ttgaggccct
     5341 gctggaggcg ggccaggcgc gggatgccca ggacgtccag gccagccagg cggaggctga
     5401 ccagcagcag actcgcctca aggagctgga gtcccaggtg tcgggtctgg agaaggaggc
     5461 catcgagctc agggaggccg tcgagcagca gaaagtgaag aacaatgacc tccgggagaa
     5521 gaactggaag gccatggagg cactggccac ggccgagcag gcctgcaagg agaagctgct
     5581 ctccctgacc caggccaagg aggaatcgga gaagcagctc tgtctgattg aggcgcagac
     5641 catggaggcc ctgctggctc tgctcccaga actctctgtc ttggcacaac agaattacac
     5701 cgagtggctg caggatctca aagagaaagg ccccacgctg ctgaagcacc cgccagctcc
     5761 cgcggagccc tcctcggacc tggcctccaa gttgagggag gccgaggaga cgcagagcac
     5821 actgcaggcc gagtgtgacc agtaccgcag catcctggcg gagacggagg gcatgctcag
     5881 agacctgcag aagagcgtgg aggaggagga gcaggtgtgg agggccaagg tgggcgccgc
     5941 agaggaggag ctccagaagt cccgggtcac agtgaagcat ctcgaagaga ttgtagagaa
     6001 gctaaaagga gaacttgaaa gttcggacca ggtgagggag cacacgtcgc atttggaggc
     6061 agagctggaa aagcacatgg cggccgccag cgccgagtgc cagaactacg ccaaggaggt
     6121 ggcagggctg aggcaacttc tcctagaatc tcaatctcag ctcgatgccg ccaagagcga
     6181 agcccagaaa cagagcgatg agcttgccct ggtcaggcag cagttgagtg aaatgaagag
     6241 ccacgtagag gatggtgaca tagctggggc cccagcttcc tccccagagg cgcccccagc
     6301 cgagcaggac cccgttcagc tgaagacgca gctggagtgg acagaagcca tcctggagga
     6361 tgagcagaca cagcggcaga agctcacggc cgagtttgag gaggctcaga cctcggcatg
     6421 tcggttacaa gaagaattgg agaagctccg cacagccggc cccctagagt cttcagaaac
     6481 agaggaggcc tcacagctga aggagagact agaaaaagag aagaagttaa caagtgacct
     6541 ggggcgcgcc gccacgagac tgcaggagct tctgaagacg acccaggagc agctggcaag
     6601 ggagaaggac acggtgaaga agctgcagga acagctggaa aaggcagagg acggcagcag
     6661 ctcaaaggag ggcacctctg tctgaagcgg ccgcgactct agagtcgacc tgcaggcatg
     6721 caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     6781 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6841 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6901 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6961 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7021 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7081 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7141 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7201 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg ggcgcccccc
     7261 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
     7321 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
     7381 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
     7441 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
     7501 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
     7561 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
     7621 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
     7681 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     7741 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
     7801 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
     7861 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
     7921 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc
     7981 tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc
     8041 gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc
     8101 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta
     8161 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg
     8221 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt
     8281 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa
     8341 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa
     8401 ttctggccgt ttttggcttt tttgttagac gaagcttggt accgagctcg gatcaattcg
     8461 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     8521 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     8581 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     8641 ccaccctcgt gaccaccttg acctacggcg tgcagtgctt cgcccgctac cccgaccaca
     8701 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     8761 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     8821 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     8881 ggcacaagct ggagtacaac tacaacagcc acaaggtcta tatcaccgcc gacaagcaga
     8941 agaacggcat caaggtgaac ttcaagaccc gccacaacat cgaggacggc agcgtgcagc
     9001 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     9061 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     9121 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     9181 agtaactcga gggaattaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     9241 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     9301 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     9361 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     9421 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     9481 gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct
     9541 atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg
     9601 ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct
     9661 atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc
     9721 tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
     9781 ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     9841 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     9901 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     9961 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
    10021 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta
    10081 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
    10141 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
    10201 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
    10261 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    10321 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    10381 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    10441 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    10501 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    10561 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    10621 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    10681 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    10741 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10801 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10861 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10921 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10981 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    11041 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    11101 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    11161 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    11221 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    11281 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    11341 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    11401 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11461 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    11521 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11581 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    11641 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    11701 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    11761 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11821 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11881 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11941 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    12001 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    12061 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    12121 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    12181 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    12241 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    12301 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    12361 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    12421 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    12481 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    12541 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    12601 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
    12661 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
    12721 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
    12781 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
    12841 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
    12901 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
    12961 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
    13021 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
    13081 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
    13141 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
    13201 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
    13261 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
    13321 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
    13381 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
    13441 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
    13501 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
    13561 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
    13621 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
    13681 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
    13741 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
    13801 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
    13861 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
    13921 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
    13981 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
    14041 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
    14101 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
    14161 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
    14221 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
    14281 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
    14341 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
    14401 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    14461 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    14521 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    14581 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
    14641 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
    14701 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
    14761 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
    14821 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
    14881 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
    14941 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
    15001 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgc
//
