LOCUS       sequence-447140-       11492 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmid for expression of AT2G40380.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11492)
  TITLE     Roston lab plasmids
REFERENCE   2  (bases 1 to 11492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUBC(k)-AT2G40380-mKOk
FEATURES             Location/Qualifiers
     source          1..11492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1339..1363)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2344..2362)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2345..2362)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2391..2400
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2430..2449)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2431..2449)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2456..2473)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2456..2472)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2465..2487)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2630..2813
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(2814..2855,2856..2864,2865..3635)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2895..2914)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3505..3524
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4260..4512
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4605..4629
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5150..5941
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6187..6775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6676..6695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6929..6946
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6961..7101
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7027..7046)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7187..7209
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7445..7639
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7705..8778)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9207..9836)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11167..11191
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11356..11377
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11392..11397,11398..11415,11416..11422)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11430..11446
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11435..11457
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11454..11470
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11454..11470
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat gtcttcttct ccggcgattc
      721 ttcccgtcac caaccagcaa gcagccactc aatctcaacc tccaatcaat tctcacgcct
      781 tccgtacttt cctctcacgc ctctcgtcct ctctccgcga aagcctctct caacgccgtc
      841 catggctaga actcgtagat cggagctcct tcgctagacc tgactctctc accgattcct
      901 tctcccggat ccgtaagaat ctcgcttact tcaaagtcaa ttactccgca attgtctcgt
      961 tagtccttgc tttctccctc ctctcgcatc cattctcgct tctcgtcctc ctctctctct
     1021 taggctcctg gatgtttctc tacctcttcc gatcttcaga tcagcctttg gttctctttg
     1081 gtcgtagttt ctcagatcgt gagactttgc ttggtcttgt tttgactacg attgtggttg
     1141 tgtttatgac gagtgttgga tctttgttaa catcggcgtt aacgattgga atcgcgattg
     1201 tttgcttaca tggtgcgttt agggttcctg atgatttgtt cttggatgaa caagagcctg
     1261 ctaatgctgg tttactctcg ttcattggta attctgctgc tacttctgct gctgcttctg
     1321 ttgttgcagg acgagttcac ccagctttct tgtacaaagt ggtgatggga cgtccgcgga
     1381 gatctacgcg tgtcgactcg agatatccaa ctagtatggt gagcgttatc aagcctgaga
     1441 tgaagatgag gtactacatg gacggaagcg tgaacggaca cgagttcact attgaaggcg
     1501 aaggtactgg aaggccttac gagggacatc aagagatgac tctcagagtg accatggctg
     1561 aaggtggacc tatgcctttc gctttcgatc tcgtgtctca cgtgttctgc tacggacaca
     1621 gagtgttcac taagtaccct gaagagatcc cggactactt caagcaagct ttccctgagg
     1681 gactctcttg ggagagatct ttggaattcg aggacggtgg atccgcttct gtgtctgctc
     1741 atatctctct caggggcaac accttctacc acaagagcaa gtttaccggg gtgaacttcc
     1801 ctgctgatgg accgatcatg cagaaccagt ctgttgattg ggagccgtct actgagaaga
     1861 tcaccgcttc tgatggtgtg ctcaagggtg atgtgaccat gtaccttaag ctcgaaggtg
     1921 gtggaaacca caagtgccag ttcaagacta cctacaaggc cgccaaagaa atcctcgaga
     1981 tgcctggtga tcactacatc ggacataggc tcgtgagaaa gaccgaggga aacatcactg
     2041 agcaggttga ggatgctgtg gctcattctc aattgttata agcggccatg ctagagtccg
     2101 caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt
     2161 gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc
     2221 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
     2281 aattcctaaa accaaaatcc agtgacctgc aggcatgcaa gctagcttac tagtgatgca
     2341 tattctatag tgtcacctaa atctgcggcc gcactagtga tatcccgcgg ccatggcggc
     2401 cgggagcatg cgacgtcggg cccaattcgc cctatagtga gtcgtattac aattcactgg
     2461 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     2521 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     2581 cccaacagtt gcgcagcctg aatggcgaat ggaaattgta agcgttaatg ggtttctgga
     2641 gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa gtcgcctaag
     2701 gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt ccataaattc
     2761 ccctcggtat ccaattagag tctcatattc actctcaatc caaataatct gcaatggcaa
     2821 ttaccttatc cgcaacttct ttacctattt ccgcccggat ccgggcaggt tctccggccg
     2881 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     2941 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     3001 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     3061 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     3121 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     3181 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     3241 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     3301 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     3361 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     3421 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     3481 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     3541 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     3601 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     3661 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     3721 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     3781 tctcatgctg gagttcttcg cccaccccga tccaacactt acgtttgcaa cgtccaagag
     3841 caaatagacc acgaacgccg gaaggttgcc gcagcgtgtg gattgcgtct caattctctc
     3901 ttgcaggaat gcaatgatga atatgatact gactatgaaa ctttgaggga atactgccta
     3961 gcaccgtcac ctcataacgt gcatcatgca tgccctgaca acatggaaca tcgctatttt
     4021 tctgaagaat tatgctcgtt ggaggatgtc gcggcaattg cagctattgc caaaatcgaa
     4081 atacccctca cgcatgcatt catcaatatt attcatgcgg ggaaaggcaa gattaatcca
     4141 actggcaaat catccagcgt gattggtaac ttcagttcca gcgacttgat tcgttttggt
     4201 gctacccacg ttttcaataa ggacgagatg gtggagtaaa gaaggagtgc gtcgaagcag
     4261 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     4321 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     4381 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     4441 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     4501 tgttactaga tcgaccggca tgcaagctga taattcaatt cggcgttaat tcagtacatt
     4561 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     4621 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
     4681 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
     4741 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
     4801 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
     4861 gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     4921 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     4981 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     5041 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     5101 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
     5161 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     5221 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     5281 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     5341 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     5401 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     5461 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     5521 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     5581 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     5641 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     5701 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     5761 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     5821 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     5881 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     5941 atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg
     6001 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat
     6061 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa
     6121 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6181 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6241 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6301 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6361 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6421 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6481 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6541 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6601 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6661 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6721 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6781 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6841 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6901 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6961 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     7021 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     7081 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     7141 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     7201 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg
     7261 ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag
     7321 gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag
     7381 ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga
     7441 cagttatgca caggccaggc gggttttaag agttttaata agttttaaag agttttaggc
     7501 ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat
     7561 gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc
     7621 aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat
     7681 ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt
     7741 gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc
     7801 cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc
     7861 ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc
     7921 ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc
     7981 ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc
     8041 agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg
     8101 gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt
     8161 acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt
     8221 ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa
     8281 cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg
     8341 ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc
     8401 ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc
     8461 acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac
     8521 gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat
     8581 cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc
     8641 ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc
     8701 ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga
     8761 tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc
     8821 cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat
     8881 tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa
     8941 attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga
     9001 tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc
     9061 ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa
     9121 cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt
     9181 gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc
     9241 gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg
     9301 gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg
     9361 tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac
     9421 acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc
     9481 accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag
     9541 tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact
     9601 acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg
     9661 acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga
     9721 tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt
     9781 gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt
     9841 tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca
     9901 agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg
     9961 ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca
    10021 gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg
    10081 cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc
    10141 ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc
    10201 ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag
    10261 tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg
    10321 gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg
    10381 ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat
    10441 gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc
    10501 cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg
    10561 gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt
    10621 cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc
    10681 gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc
    10741 ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc
    10801 agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt
    10861 tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg
    10921 cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa
    10981 gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttaaagta ctttaaagta
    11041 ctttaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg
    11101 aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc
    11161 tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga
    11221 aacgacaatc tgatccaagc tcaagctgct ctagccaata cgcaaaccgc ctctccccgc
    11281 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    11341 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    11401 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    11461 cagctatgac atgattacga attcgagctc gg
//
