LOCUS       sequence-447302-       11855 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmid for expression of AT1G50260.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11855)
  TITLE     Roston lab plasmids
REFERENCE   2  (bases 1 to 11855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUBC(b)-AT1G50260-mKOk
FEATURES             Location/Qualifiers
     source          1..11855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2725..2749)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3831..4010
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4052..4603
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      4623..4875
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4968..4992
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5513..6304
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6550..7138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7039..7058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7292..7309
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7324..7464
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7390..7409)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7550..7572
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7808..8002
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8068..9141)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9570..10199)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11530..11554
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11719..11740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11755..11760,11761..11778,11779..11785)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11793..11809
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11798..11820
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11817..11833
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11817..11833
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat gattccacaa acttcttctt
      721 cgaattttga tttccgatta cctgttgatg tgtcttgcac tctgctctgt ccatgctcta
      781 atgagctccg cgcggttttt tttacaagga gtcgggttct gcgtccgagc gtcaaaattt
      841 cgaactttag gtttatttcc tgtggatttc gtgggaattc gaaaaaccta agattgacgg
      901 attctagtag aaaggctgca aataggtttg taattgctag gtttacgaat gaatttgaag
      961 acgagggtag ttcgaaagag tccaatgacc aagccaattt cagtaacttt agggaagatc
     1021 cgatagtgga taagcttagg acccagttag gtgtaatcca tccacttccg tcaccgacca
     1081 ttaatcgtag tgtaattagt ctattcgtct tcttcttttt cgttggtgtt gcttttgata
     1141 agctatggac ttggagaaag aggcaaagag aaacaggtgg agatggaaac caacgaggag
     1201 tagggccatg gccacaggtt cccaccagct tctctttgtt cctagagaag gatttgcaaa
     1261 ggaaagagtc cgtggagtgg gtcaacatgg tgttagggaa gctctggaaa gtgtatagag
     1321 cgggtattga gaattggctc gttggtttgt tacagcctgt gattgatgac ttgaaaaagc
     1381 ctgattatgt gcagagagtt gagattaagc agttttcttt aggggatgag ccgttgtctg
     1441 tcagaaatgt tgagaggaag acatctcgcc gtgccaatga cttgcagtac caaattggcc
     1501 ttcggtatac tggcggtgca cgcatgttgt taatgctctc tttgaaattc ggtgttatcc
     1561 caatagttgt tccagttggt gtccgagatt ttgacattga tggtgagctt tgggtcaaac
     1621 taagattaat accgacacaa ccttgggtgg gagctgtatc atgttcgttt gtatcacttc
     1681 caaaggtcac atttcaactt gcagcattca gattgtttaa tctaatggga attcccgttc
     1741 tatcaatgtt cttgaccaaa ctgctgactg tggatttgcc tcgattattt gtccgaccga
     1801 agaaaattgt cttggacttt cagaaaggaa aagctgttgg acctgtttta gaagacctga
     1861 aatcaggaga aatgcaggaa ggcaataagg attttgtcgg ggaactatct gttactcttg
     1921 ttgacgccca gaaacttcga tacatgtttt ttggtaaaac ggatccatat gcaattctca
     1981 gattagggga tcaagttatc cgtagtaaaa ggaacagtca aactactgtc attggggctc
     2041 ctggtcagcc aatttggaat caggactttc aattccttgt ttcaaatcct agagagcaag
     2101 tattacaaat tgaggtcaat gatcgtcttg ggtttgctga tatggctatt ggtactggag
     2161 aggttgatct cagatttctg caagatacgg ttcctacaga cagaattgtg gttttgcgtg
     2221 gtggttggag tttgtttgga aagggatctg cgggtgagat actgctacgg cttacataca
     2281 aatcatatgt ggaggaggaa gaagatgaca agaccaatgt caaagccatt gatacttctg
     2341 acgatgaaat gtctgattca gaagaacttg gctcatttgt gcggaaggga aagctttcgt
     2401 cagatgatat tgatcaagag tcatttatga acgtgttgtc tgcattgatt gtgagtgagg
     2461 agtttcaagg catagtttca tctgaagcta gagacggtat aattgatggt ggtgactcac
     2521 ttggggcacc agtaccttct aagccagata ctagtaaggg gtctgaaaga aacgcagatg
     2581 tatcaaattt ggatttgtta gttgcaaatt ctggtagagg agctggcggt gatggaggat
     2641 tagcattact gtggttcggt attatcacgg gtatattggt tcttgttgct atcaacatgg
     2701 aaggctcaag tttcttcaac ccgcacccag ctttcttgta caaagtggtg atgggacgtc
     2761 cgcggagatc tacgcgtgtc gactcgagat atccaactag tatggtgagc gttatcaagc
     2821 ctgagatgaa gatgaggtac tacatggacg gaagcgtgaa cggacacgag ttcactattg
     2881 aaggcgaagg tactggaagg ccttacgagg gacatcaaga gatgactctc agagtgacca
     2941 tggctgaagg tggacctatg cctttcgctt tcgatctcgt gtctcacgtg ttctgctacg
     3001 gacacagagt gttcactaag taccctgaag agatcccgga ctacttcaag caagctttcc
     3061 ctgagggact ctcttgggag agatctttgg aattcgagga cggtggatcc gcttctgtgt
     3121 ctgctcatat ctctctcagg ggcaacacct tctaccacaa gagcaagttt accggggtga
     3181 acttccctgc tgatggaccg atcatgcaga accagtctgt tgattgggag ccgtctactg
     3241 agaagatcac cgcttctgat ggtgtgctca agggtgatgt gaccatgtac cttaagctcg
     3301 aaggtggtgg aaaccacaag tgccagttca agactaccta caaggccgcc aaagaaatcc
     3361 tcgagatgcc tggtgatcac tacatcggac ataggctcgt gagaaagacc gagggaaaca
     3421 tcactgagca ggttgaggat gctgtggctc attctcaatt gttataagcg gccatgctag
     3481 agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa
     3541 taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg
     3601 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
     3661 atttctaatt cctaaaacca aaatccagtg acctgcaggc atgcgacgtc gggccctcta
     3721 gaggatcccc gggtaccgcg aattatcgat catgagcgga gaattaaggg agtcacgtta
     3781 tgacccccgc cgatgacgcg ggacaagccg ttttacgttt ggaactgaca gaaccgcaac
     3841 gttgaaggag ccactgagcc gcgggtttct ggagtttaat gagctaagca catacgtcag
     3901 aaaccattat tgcgcgttca aaagtcgcct aaggtcacta tcagctagca aatatttctt
     3961 gtcaaaaatg ctccactgac gttccataaa ttcccctcgg tatccaatta gagtctcata
     4021 ttcactctca actcgatcga ggggatctac catgagccca gaacgacgcc cggccgacat
     4081 ccgccgtgcc accgaggcgg acatgccggc ggtctgcacc atcgtcaacc actacatcga
     4141 gacaagcacg gtcaacttcc gtaccgagcc gcaggaaccg caggagtgga cggacgacct
     4201 cgtccgtctg cgggagcgct atccctggct cgtcgccgag gtggacggcg aggtcgccgg
     4261 catcgcctac gcgggtccct ggaaggcacg caacgcctac gactggacgg ccgagtcgac
     4321 cgtgtacgtc tccccccgcc accagcggac gggactgggc tccacgctct acacccacct
     4381 gctgaagtcc ctggaggcac agggcttcaa gagcgtggtc gctgtcatcg ggctgcccaa
     4441 cgacccgagc gtgcgcatgc acgaggcgct cggatatgcc ccccgcggca tgctgcgggc
     4501 ggccggcttc aagcacggga actggcatga cgtgggtttc tggcagctgg acttcagcct
     4561 gccggtgccg ccccgtccgg tcctgcccgt caccgaaatc tgatgacccc tagagtcaag
     4621 cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     4681 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     4741 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     4801 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     4861 ctatgttact agatcgaccg gcatgcaagc tgataattca attcggcgtt aattcagtac
     4921 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     4981 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     5041 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
     5101 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
     5161 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
     5221 cctgtgatca attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     5281 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     5341 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     5401 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     5461 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga
     5521 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     5581 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     5641 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     5701 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     5761 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     5821 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     5881 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     5941 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     6001 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     6061 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     6121 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     6181 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     6241 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     6301 ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     6361 gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt
     6421 tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac
     6481 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6541 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6601 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6661 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     6721 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6781 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6841 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6901 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6961 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7021 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7081 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7141 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7201 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7261 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7321 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     7381 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     7441 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     7501 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     7561 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg
     7621 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
     7681 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
     7741 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
     7801 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
     7861 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
     7921 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
     7981 cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc
     8041 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
     8101 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
     8161 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
     8221 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
     8281 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
     8341 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
     8401 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
     8461 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
     8521 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
     8581 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
     8641 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
     8701 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
     8761 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
     8821 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
     8881 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
     8941 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
     9001 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
     9061 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
     9121 ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag
     9181 ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
     9241 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
     9301 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
     9361 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
     9421 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
     9481 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
     9541 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
     9601 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
     9661 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
     9721 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
     9781 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
     9841 tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg
     9901 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
     9961 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
    10021 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
    10081 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
    10141 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
    10201 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
    10261 gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag
    10321 cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat
    10381 gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct
    10441 ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca
    10501 tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc
    10561 gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc
    10621 aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt
    10681 gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg
    10741 gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc
    10801 gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc
    10861 ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc
    10921 ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct
    10981 ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg
    11041 gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag
    11101 ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag
    11161 gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta
    11221 ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg
    11281 tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca
    11341 gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa
    11401 gtactttaaa gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg
    11461 acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt
    11521 ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg
    11581 ggaaacgaca atctgatcca agctcaagct gctctagcca atacgcaaac cgcctctccc
    11641 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
    11701 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
    11761 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
    11821 aaacagctat gacatgatta cgaattcgag ctcgg
//
