LOCUS       sequence-447310-       11522 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmid for expression of AT5G01640.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11522)
  TITLE     Roston lab plasmids
REFERENCE   2  (bases 1 to 11522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUBC(k)-AT5G01640-mKOk
FEATURES             Location/Qualifiers
     source          1..11522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1369..1393)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2374..2392)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2375..2392)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2421..2430
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2460..2479)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2461..2479)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2486..2503)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2486..2502)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2495..2517)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2660..2843
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(2844..2885,2886..2894,2895..3665)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2925..2944)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3535..3554
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4290..4542
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4635..4659
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5180..5971
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6217..6805
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6706..6725
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6959..6976
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6991..7131
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7057..7076)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7217..7239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7475..7669
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7735..8808)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9237..9866)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11197..11221
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11386..11407
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11422..11427,11428..11445,11446..11452)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11460..11476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11465..11487
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11484..11500
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11484..11500
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat ggtgtcaaca aatccccctg
      721 ttcttccaat ttccaccacc gcaacagaca ccaccaatca gcctccgatt gtaaccgctg
      781 tggtggaatc gcaacctcca gtcgtccgtg catttgtcaa cggtgtgacc gagacggtct
      841 gtggtggtct atctcgtagc cgtccgtggt cagagctcct cgatcgatcc gctttcacaa
      901 agcctgactc tctctccgag gccgggacgc gattccggaa aaattcctct tatttcaggg
      961 tgaattacgt ttgcattgtc gctctgatcc ttggtttctc tcttctcgcc cacccattct
     1021 ctctcatcct cctcctatgt ctcgccgctt cctggctctt cctctacctc ttccgtccat
     1081 cagatcgtcc tcttatcctc ttcggccgat ctttctccga atacgaaact ctcggtggcc
     1141 taatcctctc cacgatcgca gtgatcttct tcacaagcgt cggatctgta ttgatatccg
     1201 ctctcatgat cggaatagca acaatctgtg tccatggcgc ctttagagct cccgatgatc
     1261 tcttcctcga cgaacaagat catgccgcct ccggattcct ctcattcatc ggtgttcccg
     1321 cgatcccctc tgtagcaccc tctgcctcct cagcggcttc ccccgtgcac ccagctttct
     1381 tgtacaaagt ggtgatggga cgtccgcgga gatctacgcg tgtcgactcg agatatccaa
     1441 ctagtatggt gagcgttatc aagcctgaga tgaagatgag gtactacatg gacggaagcg
     1501 tgaacggaca cgagttcact attgaaggcg aaggtactgg aaggccttac gagggacatc
     1561 aagagatgac tctcagagtg accatggctg aaggtggacc tatgcctttc gctttcgatc
     1621 tcgtgtctca cgtgttctgc tacggacaca gagtgttcac taagtaccct gaagagatcc
     1681 cggactactt caagcaagct ttccctgagg gactctcttg ggagagatct ttggaattcg
     1741 aggacggtgg atccgcttct gtgtctgctc atatctctct caggggcaac accttctacc
     1801 acaagagcaa gtttaccggg gtgaacttcc ctgctgatgg accgatcatg cagaaccagt
     1861 ctgttgattg ggagccgtct actgagaaga tcaccgcttc tgatggtgtg ctcaagggtg
     1921 atgtgaccat gtaccttaag ctcgaaggtg gtggaaacca caagtgccag ttcaagacta
     1981 cctacaaggc cgccaaagaa atcctcgaga tgcctggtga tcactacatc ggacataggc
     2041 tcgtgagaaa gaccgaggga aacatcactg agcaggttga ggatgctgtg gctcattctc
     2101 aattgttata agcggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct
     2161 atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg
     2221 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     2281 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc
     2341 aggcatgcaa gctagcttac tagtgatgca tattctatag tgtcacctaa atctgcggcc
     2401 gcactagtga tatcccgcgg ccatggcggc cgggagcatg cgacgtcggg cccaattcgc
     2461 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     2521 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     2581 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     2641 ggaaattgta agcgttaatg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa
     2701 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc
     2761 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc
     2821 actctcaatc caaataatct gcaatggcaa ttaccttatc cgcaacttct ttacctattt
     2881 ccgcccggat ccgggcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     2941 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     3001 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     3061 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     3121 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     3181 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     3241 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     3301 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     3361 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     3421 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     3481 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     3541 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     3601 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     3661 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     3721 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     3781 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccga
     3841 tccaacactt acgtttgcaa cgtccaagag caaatagacc acgaacgccg gaaggttgcc
     3901 gcagcgtgtg gattgcgtct caattctctc ttgcaggaat gcaatgatga atatgatact
     3961 gactatgaaa ctttgaggga atactgccta gcaccgtcac ctcataacgt gcatcatgca
     4021 tgccctgaca acatggaaca tcgctatttt tctgaagaat tatgctcgtt ggaggatgtc
     4081 gcggcaattg cagctattgc caaaatcgaa atacccctca cgcatgcatt catcaatatt
     4141 attcatgcgg ggaaaggcaa gattaatcca actggcaaat catccagcgt gattggtaac
     4201 ttcagttcca gcgacttgat tcgttttggt gctacccacg ttttcaataa ggacgagatg
     4261 gtggagtaaa gaaggagtgc gtcgaagcag atcgttcaaa catttggcaa taaagtttct
     4321 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     4381 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     4441 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     4501 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgaccggca tgcaagctga
     4561 taattcaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     4621 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     4681 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     4741 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     4801 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     4861 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
     4921 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     4981 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     5041 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     5101 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     5161 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     5221 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     5281 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     5341 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     5401 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     5461 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     5521 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     5581 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     5641 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     5701 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     5761 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     5821 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     5881 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     5941 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     6001 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca
     6061 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca
     6121 caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6181 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6241 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6301 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6361 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6421 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6481 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6541 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6601 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6661 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6721 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6781 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6841 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6901 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6961 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     7021 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     7081 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     7141 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7201 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7261 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
     7321 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
     7381 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     7441 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     7501 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     7561 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     7621 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     7681 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     7741 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     7801 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     7861 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     7921 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     7981 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     8041 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     8101 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     8161 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     8221 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     8281 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
     8341 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
     8401 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
     8461 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
     8521 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
     8581 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
     8641 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
     8701 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
     8761 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
     8821 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
     8881 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
     8941 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
     9001 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
     9061 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
     9121 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
     9181 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
     9241 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
     9301 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
     9361 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
     9421 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
     9481 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
     9541 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
     9601 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
     9661 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
     9721 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
     9781 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
     9841 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
     9901 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
     9961 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    10021 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
    10081 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    10141 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    10201 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    10261 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    10321 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    10381 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    10441 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    10501 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    10561 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    10621 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    10681 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    10741 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    10801 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    10861 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    10921 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    10981 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    11041 gatcaaagta ctttaaagta ctttaaagta ctttaaagta ctttgatccc gaggggaacc
    11101 ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa
    11161 tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc
    11221 aaacactgat agtttaaact gaaggcggga aacgacaatc tgatccaagc tcaagctgct
    11281 ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    11341 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    11401 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    11461 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgagctc
    11521 gg
//
