LOCUS       sequence-447314-       11501 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmid for expression of AT5G07110.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11501)
  TITLE     Roston lab plasmids
REFERENCE   2  (bases 1 to 11501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUBC(k)-AT5G07110-mKOk
FEATURES             Location/Qualifiers
     source          1..11501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1348..1372)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2353..2371)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2354..2371)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2400..2409
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2439..2458)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2440..2458)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2465..2482)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2465..2481)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2474..2496)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2639..2822
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(2823..2864,2865..2873,2874..3644)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2904..2923)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3514..3533
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4269..4521
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4614..4638
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5159..5950
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6196..6784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6685..6704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6938..6955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6970..7110
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7036..7055)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7196..7218
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7454..7648
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7714..8787)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9216..9845)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11176..11200
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11365..11386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11401..11406,11407..11424,11425..11431)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11439..11455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11444..11466
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11463..11479
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11463..11479
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat ggcttctcct cttctcccaa
      721 cttcgactac tcctgatcaa cttcccggcg gcgatccaca gttgctgagc tctctacgcg
      781 tcctcttgtc ccgcgttcta gccaccgtcc gtcacgcttc tgcagatgcc agaccctggg
      841 cagagctcgt tgaccggtca gcgttttccc ggccaccatc gctctcggag gcaacgtcac
      901 gagtaaggaa gaacttttcc tatttccgag ccaattacat aaccttagtg gcaatcttac
      961 tcgccgcgtc tctgctcacg caccctttcg ctctcttcct cctcgcatcg ctggccgctt
     1021 cttggctttt cctctacttt ttccgtccgg cggatcagcc gttggtcatt ggaggacgca
     1081 cgttctccga tcttgagacg ctagggatac tctgcctgtc cactgtggtg gtgatgttca
     1141 tgaccagcgt tggatcgctc ttgatgtcca ctctagcagt tgggatcatg ggcgtggcca
     1201 tccacggagc gtttcgtgct cccgaagacc tgtttcttga agaacaagaa gccattggat
     1261 ctggactttt cgcattcttc aacaacaatg cctctaatgc agctgccgct gccatagcca
     1321 cctcagcaat gtcacgcgtt cgagtccacc cagctttctt gtacaaagtg gtgatgggac
     1381 gtccgcggag atctacgcgt gtcgactcga gatatccaac tagtatggtg agcgttatca
     1441 agcctgagat gaagatgagg tactacatgg acggaagcgt gaacggacac gagttcacta
     1501 ttgaaggcga aggtactgga aggccttacg agggacatca agagatgact ctcagagtga
     1561 ccatggctga aggtggacct atgcctttcg ctttcgatct cgtgtctcac gtgttctgct
     1621 acggacacag agtgttcact aagtaccctg aagagatccc ggactacttc aagcaagctt
     1681 tccctgaggg actctcttgg gagagatctt tggaattcga ggacggtgga tccgcttctg
     1741 tgtctgctca tatctctctc aggggcaaca ccttctacca caagagcaag tttaccgggg
     1801 tgaacttccc tgctgatgga ccgatcatgc agaaccagtc tgttgattgg gagccgtcta
     1861 ctgagaagat caccgcttct gatggtgtgc tcaagggtga tgtgaccatg taccttaagc
     1921 tcgaaggtgg tggaaaccac aagtgccagt tcaagactac ctacaaggcc gccaaagaaa
     1981 tcctcgagat gcctggtgat cactacatcg gacataggct cgtgagaaag accgagggaa
     2041 acatcactga gcaggttgag gatgctgtgg ctcattctca attgttataa gcggccatgc
     2101 tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca
     2161 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat
     2221 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     2281 aaaatttcta attcctaaaa ccaaaatcca gtgacctgca ggcatgcaag ctagcttact
     2341 agtgatgcat attctatagt gtcacctaaa tctgcggccg cactagtgat atcccgcggc
     2401 catggcggcc gggagcatgc gacgtcgggc ccaattcgcc ctatagtgag tcgtattaca
     2461 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     2521 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     2581 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatgg
     2641 gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag
     2701 tcgcctaagg tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc
     2761 cataaattcc cctcggtatc caattagagt ctcatattca ctctcaatcc aaataatctg
     2821 caatggcaat taccttatcc gcaacttctt tacctatttc cgcccggatc cgggcaggtt
     2881 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     2941 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     3001 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     3061 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     3121 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     3181 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     3241 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     3301 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     3361 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     3421 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     3481 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     3541 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     3601 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     3661 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     3721 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     3781 gcgcggggat ctcatgctgg agttcttcgc ccaccccgat ccaacactta cgtttgcaac
     3841 gtccaagagc aaatagacca cgaacgccgg aaggttgccg cagcgtgtgg attgcgtctc
     3901 aattctctct tgcaggaatg caatgatgaa tatgatactg actatgaaac tttgagggaa
     3961 tactgcctag caccgtcacc tcataacgtg catcatgcat gccctgacaa catggaacat
     4021 cgctattttt ctgaagaatt atgctcgttg gaggatgtcg cggcaattgc agctattgcc
     4081 aaaatcgaaa tacccctcac gcatgcattc atcaatatta ttcatgcggg gaaaggcaag
     4141 attaatccaa ctggcaaatc atccagcgtg attggtaact tcagttccag cgacttgatt
     4201 cgttttggtg ctacccacgt tttcaataag gacgagatgg tggagtaaag aaggagtgcg
     4261 tcgaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     4321 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     4381 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     4441 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     4501 tgtcatctat gttactagat cgaccggcat gcaagctgat aattcaattc ggcgttaatt
     4561 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     4621 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     4681 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     4741 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     4801 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     4861 ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     4921 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     4981 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     5041 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     5101 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     5161 gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     5221 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     5281 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     5341 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     5401 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     5461 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     5521 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     5581 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     5641 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     5701 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     5761 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     5821 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     5881 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     5941 cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta
     6001 actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc
     6061 tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg
     6121 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6181 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6241 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6301 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     6361 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6421 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6481 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6541 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6601 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6661 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6721 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6781 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6841 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6901 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6961 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     7021 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     7081 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     7141 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     7201 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
     7261 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
     7321 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
     7381 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
     7441 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
     7501 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
     7561 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
     7621 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
     7681 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
     7741 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
     7801 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
     7861 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
     7921 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
     7981 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
     8041 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
     8101 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
     8161 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
     8221 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
     8281 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
     8341 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
     8401 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
     8461 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
     8521 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
     8581 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
     8641 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
     8701 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
     8761 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
     8821 ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
     8881 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
     8941 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
     9001 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
     9061 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
     9121 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
     9181 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
     9241 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
     9301 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
     9361 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
     9421 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
     9481 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
     9541 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
     9601 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
     9661 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
     9721 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
     9781 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
     9841 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
     9901 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
     9961 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
    10021 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
    10081 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
    10141 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
    10201 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
    10261 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
    10321 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
    10381 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
    10441 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
    10501 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
    10561 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
    10621 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
    10681 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
    10741 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
    10801 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
    10861 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
    10921 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
    10981 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttaaagtac
    11041 tttaaagtac tttaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
    11101 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
    11161 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
    11221 aaggcgggaa acgacaatct gatccaagct caagctgctc tagccaatac gcaaaccgcc
    11281 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
    11341 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    11401 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    11461 cacaggaaac agctatgaca tgattacgaa ttcgagctcg g
//
