LOCUS       sequence-447323-       11648 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmid for expression of AT1G77730.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11648)
  TITLE     Roston lab plasmids
REFERENCE   2  (bases 1 to 11648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUBC(k)-AT1G77730-mKOk
FEATURES             Location/Qualifiers
     source          1..11648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1495..1519)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2500..2518)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2501..2518)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2547..2556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2586..2605)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2587..2605)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2612..2629)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2612..2628)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2621..2643)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2786..2969
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(2970..3011,3012..3020,3021..3791)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3051..3070)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3661..3680
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4416..4668
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4761..4785
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5306..6097
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6343..6931
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6832..6851
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7085..7102
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7117..7257
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7183..7202)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7343..7365
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7601..7795
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7861..8934)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9363..9992)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11323..11347
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11512..11533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11548..11553,11554..11571,11572..11578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11586..11602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11591..11613
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11610..11626
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11610..11626
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat gtcttctcca ctcgccgcaa
      721 aatcaaacct aaccacgact cgatcggctt cgttaaacat tccacattcg acacgtacca
      781 atcacaacca cgatctccgg tatagtcttt ccgctggtcc tcgtacgaac gaagatcgac
      841 cagcttctgg gaacggcgtc gccggaatcc tctataaatg ggtaaactac ggccaaggat
      901 ggaaacgacg gtggttcgta ctccaggacg gtgttttgtc gtattataga atccatggtc
      961 ccgataaaat atctctctcc gttgagatgg atcggagatc taaactgatc ggcggcgaat
     1021 ctttacggtt tatttgccga catagcaaac gcggtgatgt tcatagcccc gggaaacctc
     1081 tcggccaaat tcacctcaag gtttcatcga ttggacaaag catatcagat ggcaagagat
     1141 tcactgtatt cacgggcacg aagagtctgc atttacgagc agcaacgagc gaggatcgtg
     1201 cctcttggat cgaagcattg aaagctgtta aagaaacgtt tccaagaatg tcaaacgaag
     1261 aactaatggc atcgacgact aatgtctcag tctcgaccga taagctaagg cagagattaa
     1321 tggaagaaga ggtagatgag acaatcatca aagattgcga agacataatg aagaacaatt
     1381 tcttagcatt gcatgatgag gttatgtctc taaaacggta ccaatatcat cttatagatt
     1441 ctctcaagaa cgtcagtaac tcacctataa gacccagtaa ccaaagtttt tcacacccag
     1501 ctttcttgta caaagtggtg atgggacgtc cgcggagatc tacgcgtgtc gactcgagat
     1561 atccaactag tatggtgagc gttatcaagc ctgagatgaa gatgaggtac tacatggacg
     1621 gaagcgtgaa cggacacgag ttcactattg aaggcgaagg tactggaagg ccttacgagg
     1681 gacatcaaga gatgactctc agagtgacca tggctgaagg tggacctatg cctttcgctt
     1741 tcgatctcgt gtctcacgtg ttctgctacg gacacagagt gttcactaag taccctgaag
     1801 agatcccgga ctacttcaag caagctttcc ctgagggact ctcttgggag agatctttgg
     1861 aattcgagga cggtggatcc gcttctgtgt ctgctcatat ctctctcagg ggcaacacct
     1921 tctaccacaa gagcaagttt accggggtga acttccctgc tgatggaccg atcatgcaga
     1981 accagtctgt tgattgggag ccgtctactg agaagatcac cgcttctgat ggtgtgctca
     2041 agggtgatgt gaccatgtac cttaagctcg aaggtggtgg aaaccacaag tgccagttca
     2101 agactaccta caaggccgcc aaagaaatcc tcgagatgcc tggtgatcac tacatcggac
     2161 ataggctcgt gagaaagacc gagggaaaca tcactgagca ggttgaggat gctgtggctc
     2221 attctcaatt gttataagcg gccatgctag agtccgcaaa aatcaccagt ctctctctac
     2281 aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa
     2341 ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
     2401 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg
     2461 acctgcaggc atgcaagcta gcttactagt gatgcatatt ctatagtgtc acctaaatct
     2521 gcggccgcac tagtgatatc ccgcggccat ggcggccggg agcatgcgac gtcgggccca
     2581 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
     2641 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     2701 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     2761 gcgaatggaa attgtaagcg ttaatgggtt tctggagttt aatgagctaa gcacatacgt
     2821 cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt
     2881 cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc
     2941 atattcactc tcaatccaaa taatctgcaa tggcaattac cttatccgca acttctttac
     3001 ctatttccgc ccggatccgg gcaggttctc cggccgcttg ggtggagagg ctattcggct
     3061 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     3121 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     3181 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     3241 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     3301 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     3361 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     3421 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     3481 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     3541 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     3601 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     3661 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     3721 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     3781 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     3841 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     3901 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     3961 ccccgatcca acacttacgt ttgcaacgtc caagagcaaa tagaccacga acgccggaag
     4021 gttgccgcag cgtgtggatt gcgtctcaat tctctcttgc aggaatgcaa tgatgaatat
     4081 gatactgact atgaaacttt gagggaatac tgcctagcac cgtcacctca taacgtgcat
     4141 catgcatgcc ctgacaacat ggaacatcgc tatttttctg aagaattatg ctcgttggag
     4201 gatgtcgcgg caattgcagc tattgccaaa atcgaaatac ccctcacgca tgcattcatc
     4261 aatattattc atgcggggaa aggcaagatt aatccaactg gcaaatcatc cagcgtgatt
     4321 ggtaacttca gttccagcga cttgattcgt tttggtgcta cccacgtttt caataaggac
     4381 gagatggtgg agtaaagaag gagtgcgtcg aagcagatcg ttcaaacatt tggcaataaa
     4441 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     4501 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     4561 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     4621 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga ccggcatgca
     4681 agctgataat tcaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
     4741 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
     4801 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
     4861 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
     4921 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
     4981 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca
     5041 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     5101 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     5161 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     5221 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     5281 tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca
     5341 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     5401 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     5461 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     5521 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     5581 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     5641 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     5701 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     5761 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     5821 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     5881 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     5941 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     6001 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     6061 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa
     6121 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg
     6181 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     6241 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt
     6301 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6361 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6421 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6481 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6541 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6601 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6661 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6721 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6781 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6841 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6901 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6961 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7021 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7081 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     7141 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     7201 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     7261 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     7321 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     7381 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc
     7441 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc
     7501 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg
     7561 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt
     7621 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt
     7681 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta
     7741 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa
     7801 gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca
     7861 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
     7921 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
     7981 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
     8041 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
     8101 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
     8161 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
     8221 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
     8281 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
     8341 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
     8401 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
     8461 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
     8521 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
     8581 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
     8641 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
     8701 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg
     8761 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc
     8821 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc
     8881 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat
     8941 tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg
     9001 cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt
     9061 tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt
     9121 gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc
     9181 ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac
     9241 ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc
     9301 gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta
     9361 actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg
     9421 ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg
     9481 cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc
     9541 tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt
     9601 ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg
     9661 tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca
     9721 gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc
     9781 ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc
     9841 cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg
     9901 gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg
     9961 cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta
    10021 ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc
    10081 acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca
    10141 gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg
    10201 gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa
    10261 acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc
    10321 ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct
    10381 ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg
    10441 cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg
    10501 ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc
    10561 cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc
    10621 aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg
    10681 ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag
    10741 gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg
    10801 taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc
    10861 ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg
    10921 gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc
    10981 gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag
    11041 aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat
    11101 gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag
    11161 gggttggatc aaagtacttt aaagtacttt aaagtacttt aaagtacttt gatcccgagg
    11221 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
    11281 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
    11341 cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa
    11401 gctgctctag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    11461 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    11521 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    11581 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc
    11641 gagctcgg
//
