LOCUS       sequence-447329-       11504 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmid for expression of AT5G05380.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11504)
  TITLE     Roston lab plasmids
REFERENCE   2  (bases 1 to 11504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUBC(k)-AT5G05380-mKOk
FEATURES             Location/Qualifiers
     source          1..11504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1351..1375)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2356..2374)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2357..2374)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2403..2412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2442..2461)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2443..2461)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2468..2485)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2468..2484)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2477..2499)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2642..2825
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(2826..2867,2868..2876,2877..3647)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2907..2926)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3517..3536
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4272..4524
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4617..4641
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5162..5953
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6199..6787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6688..6707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6941..6958
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6973..7113
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7039..7058)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7199..7221
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7457..7651
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7717..8790)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9219..9848)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11179..11203
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11368..11389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11404..11409,11410..11427,11428..11434)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11442..11458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11447..11469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11466..11482
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11466..11482
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat gatggctaat cctccaacgt
      721 taccgatctc agaccattcc ggtggtggtt cccaatctca gcaacccgta tcaacaccgg
      781 cgtttcgcac gttcctctct cgcctctcgt cgtcaatccg ccaaagccta tcgcagcggc
      841 gtccatggtt agaactcgta gatcgaagcg caatttcacg tccagagtct ctcaccgatg
      901 cgtattctcg gatccggagg aatctccctt acttcaaggt aaactacgtg acgatcgtct
      961 ccttggtgct cgcgctctcg ctcctctccc atcctttctc tcttctcgtt ctcctatgcc
     1021 tcttctgcgc ttggatcttc ctctaccttt tccgtccgtc agatcaacct ctggtcgtcc
     1081 tcggccgtac gttttcagat cgtgagacgc ttggtgtttt ggtaattttg acaatcgtcg
     1141 tcgtcttttt aaccagcgtt gggtctttat tgacctctgc tttgatgatt ggtttcggaa
     1201 tcgtatgctt acacggcgcg tttagagtcc cggaggatct tttcttggat gatcaagaac
     1261 ctgctaacac cggcttattg tctttcctta gcggagccgc cacttccgcc gcagttgccg
     1321 ccgcatcaac tcctgcgtca ggtcgtgttc acccagcttt cttgtacaaa gtggtgatgg
     1381 gacgtccgcg gagatctacg cgtgtcgact cgagatatcc aactagtatg gtgagcgtta
     1441 tcaagcctga gatgaagatg aggtactaca tggacggaag cgtgaacgga cacgagttca
     1501 ctattgaagg cgaaggtact ggaaggcctt acgagggaca tcaagagatg actctcagag
     1561 tgaccatggc tgaaggtgga cctatgcctt tcgctttcga tctcgtgtct cacgtgttct
     1621 gctacggaca cagagtgttc actaagtacc ctgaagagat cccggactac ttcaagcaag
     1681 ctttccctga gggactctct tgggagagat ctttggaatt cgaggacggt ggatccgctt
     1741 ctgtgtctgc tcatatctct ctcaggggca acaccttcta ccacaagagc aagtttaccg
     1801 gggtgaactt ccctgctgat ggaccgatca tgcagaacca gtctgttgat tgggagccgt
     1861 ctactgagaa gatcaccgct tctgatggtg tgctcaaggg tgatgtgacc atgtacctta
     1921 agctcgaagg tggtggaaac cacaagtgcc agttcaagac tacctacaag gccgccaaag
     1981 aaatcctcga gatgcctggt gatcactaca tcggacatag gctcgtgaga aagaccgagg
     2041 gaaacatcac tgagcaggtt gaggatgctg tggctcattc tcaattgtta taagcggcca
     2101 tgctagagtc cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct
     2161 ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct
     2221 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
     2281 aataaaattt ctaattccta aaaccaaaat ccagtgacct gcaggcatgc aagctagctt
     2341 actagtgatg catattctat agtgtcacct aaatctgcgg ccgcactagt gatatcccgc
     2401 ggccatggcg gccgggagca tgcgacgtcg ggcccaattc gccctatagt gagtcgtatt
     2461 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     2521 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     2581 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggaaattg taagcgttaa
     2641 tgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
     2701 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
     2761 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
     2821 ctgcaatggc aattacctta tccgcaactt ctttacctat ttccgcccgg atccgggcag
     2881 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     2941 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3001 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     3061 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     3121 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     3181 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     3241 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     3301 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     3361 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     3421 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     3481 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     3541 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     3601 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     3661 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     3721 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     3781 ccagcgcggg gatctcatgc tggagttctt cgcccacccc gatccaacac ttacgtttgc
     3841 aacgtccaag agcaaataga ccacgaacgc cggaaggttg ccgcagcgtg tggattgcgt
     3901 ctcaattctc tcttgcagga atgcaatgat gaatatgata ctgactatga aactttgagg
     3961 gaatactgcc tagcaccgtc acctcataac gtgcatcatg catgccctga caacatggaa
     4021 catcgctatt tttctgaaga attatgctcg ttggaggatg tcgcggcaat tgcagctatt
     4081 gccaaaatcg aaatacccct cacgcatgca ttcatcaata ttattcatgc ggggaaaggc
     4141 aagattaatc caactggcaa atcatccagc gtgattggta acttcagttc cagcgacttg
     4201 attcgttttg gtgctaccca cgttttcaat aaggacgaga tggtggagta aagaaggagt
     4261 gcgtcgaagc agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     4321 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     4381 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     4441 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     4501 cggtgtcatc tatgttacta gatcgaccgg catgcaagct gataattcaa ttcggcgtta
     4561 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     4621 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     4681 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     4741 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     4801 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     4861 gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg
     4921 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     4981 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     5041 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     5101 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     5161 catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     5221 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     5281 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     5341 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     5401 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     5461 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     5521 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     5581 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     5641 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     5701 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     5761 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     5821 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     5881 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     5941 agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc
     6001 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa
     6061 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc
     6121 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6181 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6241 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6301 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     6361 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6421 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6481 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6541 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6601 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6661 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6721 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6781 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6841 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6901 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6961 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     7021 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     7081 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     7141 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     7201 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
     7261 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     7321 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     7381 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     7441 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     7501 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     7561 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     7621 ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc
     7681 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     7741 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     7801 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     7861 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     7921 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     7981 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     8041 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
     8101 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
     8161 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
     8221 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
     8281 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
     8341 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
     8401 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
     8461 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
     8521 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
     8581 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
     8641 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
     8701 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
     8761 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
     8821 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
     8881 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
     8941 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
     9001 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
     9061 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
     9121 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
     9181 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
     9241 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
     9301 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
     9361 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
     9421 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
     9481 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
     9541 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
     9601 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
     9661 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
     9721 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
     9781 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
     9841 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
     9901 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
     9961 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    10021 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
    10081 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
    10141 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
    10201 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
    10261 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
    10321 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    10381 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    10441 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    10501 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    10561 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    10621 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    10681 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    10741 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    10801 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    10861 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    10921 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    10981 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttaaag
    11041 tactttaaag tactttaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
    11101 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat tattctaata
    11161 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa
    11221 ctgaaggcgg gaaacgacaa tctgatccaa gctcaagctg ctctagccaa tacgcaaacc
    11281 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    11341 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    11401 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    11461 tcacacagga aacagctatg acatgattac gaattcgagc tcgg
//
