LOCUS       sequence-447337-       11480 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmid for expression of AT3G56110.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11480)
  TITLE     Roston lab plasmids
REFERENCE   2  (bases 1 to 11480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUBC(k)-AT3G56110-mKOk
FEATURES             Location/Qualifiers
     source          1..11480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1327..1351)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2332..2350)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2333..2350)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2379..2388
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2418..2437)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2419..2437)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2444..2461)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2444..2460)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2453..2475)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2618..2801
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(2802..2843,2844..2852,2853..3623)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2883..2902)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3493..3512
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4248..4500
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4593..4617
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5138..5929
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6175..6763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6664..6683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6917..6934
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6949..7089
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7015..7034)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7175..7197
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7433..7627
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7693..8766)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9195..9824)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11155..11179
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11344..11365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11380..11385,11386..11403,11404..11410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11418..11434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11423..11445
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11442..11458
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11442..11458
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat ggcaactcca ccgacgctac
      721 ctgtcaccaa tcagcaagct gttcaatctc ggcctccaat caacacacct gcttttcgta
      781 ctttcttctc acgtctctcc acctcgatcc gtgatggctt gtcacagcgt cgtccatgga
      841 cggagctcat cgatcgtagt tccatggcga gacctgaatc tctcaccgat gccttgtccc
      901 ggataaggaa gaatctcgct tacttcaagg ttaactacgt cgccatcgtc tccctcgtac
      961 tcgccttttc gcttttctct catcctctct ccctccttgt cctcatcgga ctcctaggcg
     1021 gttggatgtt tctctacctc ttccgtccct cagatcagcc actggtcgtc ttcggtcgca
     1081 ctttctctga tcgagagact ctgcttgctc ttgtgctgtc tacgatcgtc gtcgtgttta
     1141 tgacgagcgt tggatctctc ttaacctccg cgcttatgat tggtgttgca atcgtttgcg
     1201 tacacggtgc gtttgtggtt ccggatgatc tcttcttgga cgagcaagag cctgcgaatg
     1261 ctggattact ctcattcctt ggaggttctg ctacctcagc tgctgcagct gtctcaggga
     1321 gagtccaccc agctttcttg tacaaagtgg tgatgggacg tccgcggaga tctacgcgtg
     1381 tcgactcgag atatccaact agtatggtga gcgttatcaa gcctgagatg aagatgaggt
     1441 actacatgga cggaagcgtg aacggacacg agttcactat tgaaggcgaa ggtactggaa
     1501 ggccttacga gggacatcaa gagatgactc tcagagtgac catggctgaa ggtggaccta
     1561 tgcctttcgc tttcgatctc gtgtctcacg tgttctgcta cggacacaga gtgttcacta
     1621 agtaccctga agagatcccg gactacttca agcaagcttt ccctgaggga ctctcttggg
     1681 agagatcttt ggaattcgag gacggtggat ccgcttctgt gtctgctcat atctctctca
     1741 ggggcaacac cttctaccac aagagcaagt ttaccggggt gaacttccct gctgatggac
     1801 cgatcatgca gaaccagtct gttgattggg agccgtctac tgagaagatc accgcttctg
     1861 atggtgtgct caagggtgat gtgaccatgt accttaagct cgaaggtggt ggaaaccaca
     1921 agtgccagtt caagactacc tacaaggccg ccaaagaaat cctcgagatg cctggtgatc
     1981 actacatcgg acataggctc gtgagaaaga ccgagggaaa catcactgag caggttgagg
     2041 atgctgtggc tcattctcaa ttgttataag cggccatgct agagtccgca aaaatcacca
     2101 gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc
     2161 cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
     2221 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     2281 caaaatccag tgacctgcag gcatgcaagc tagcttacta gtgatgcata ttctatagtg
     2341 tcacctaaat ctgcggccgc actagtgata tcccgcggcc atggcggccg ggagcatgcg
     2401 acgtcgggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     2461 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     2521 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     2581 gcagcctgaa tggcgaatgg aaattgtaag cgttaatggg tttctggagt ttaatgagct
     2641 aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc
     2701 tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc
     2761 aattagagtc tcatattcac tctcaatcca aataatctgc aatggcaatt accttatccg
     2821 caacttcttt acctatttcc gcccggatcc gggcaggttc tccggccgct tgggtggaga
     2881 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     2941 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     3001 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     3061 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     3121 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     3181 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     3241 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     3301 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     3361 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     3421 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     3481 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     3541 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     3601 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     3661 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     3721 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     3781 gttcttcgcc caccccgatc caacacttac gtttgcaacg tccaagagca aatagaccac
     3841 gaacgccgga aggttgccgc agcgtgtgga ttgcgtctca attctctctt gcaggaatgc
     3901 aatgatgaat atgatactga ctatgaaact ttgagggaat actgcctagc accgtcacct
     3961 cataacgtgc atcatgcatg ccctgacaac atggaacatc gctatttttc tgaagaatta
     4021 tgctcgttgg aggatgtcgc ggcaattgca gctattgcca aaatcgaaat acccctcacg
     4081 catgcattca tcaatattat tcatgcgggg aaaggcaaga ttaatccaac tggcaaatca
     4141 tccagcgtga ttggtaactt cagttccagc gacttgattc gttttggtgc tacccacgtt
     4201 ttcaataagg acgagatggt ggagtaaaga aggagtgcgt cgaagcagat cgttcaaaca
     4261 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     4321 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     4381 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     4441 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     4501 gaccggcatg caagctgata attcaattcg gcgttaattc agtacattaa aaacgtccgc
     4561 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
     4621 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
     4681 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
     4741 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
     4801 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg
     4861 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     4921 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     4981 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     5041 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     5101 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
     5161 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     5221 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     5281 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     5341 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     5401 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     5461 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     5521 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     5581 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     5641 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     5701 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     5761 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     5821 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     5881 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     5941 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     6001 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     6061 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
     6121 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6181 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6241 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6301 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     6361 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6421 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6481 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6541 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6601 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6661 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6721 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6781 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6841 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6901 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     6961 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     7021 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     7081 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7141 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     7201 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     7261 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     7321 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     7381 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     7441 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     7501 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     7561 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     7621 atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     7681 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     7741 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     7801 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     7861 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     7921 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     7981 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     8041 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     8101 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     8161 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     8221 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     8281 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     8341 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     8401 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     8461 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     8521 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     8581 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     8641 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     8701 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     8761 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
     8821 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
     8881 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
     8941 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
     9001 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
     9061 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
     9121 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
     9181 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
     9241 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
     9301 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
     9361 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
     9421 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
     9481 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
     9541 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
     9601 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
     9661 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
     9721 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
     9781 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
     9841 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
     9901 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
     9961 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
    10021 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
    10081 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
    10141 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
    10201 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
    10261 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
    10321 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
    10381 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
    10441 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
    10501 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
    10561 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
    10621 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
    10681 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
    10741 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
    10801 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
    10861 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
    10921 cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact
    10981 atagcagcgg aggggttgga tcaaagtact ttaaagtact ttaaagtact ttaaagtact
    11041 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
    11101 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
    11161 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
    11221 atccaagctc aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat
    11281 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    11341 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    11401 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
    11461 gattacgaat tcgagctcgg
//
