LOCUS       sequence-447495-        2162 bp    DNA     circular SYN 15-APR-2025
DEFINITION  L3S1P32 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-L3S1P32
FEATURES             Location/Qualifiers
     source          1..2162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     64..82
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(86..129)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    132..151
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      267..324
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(348..365)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(519..1107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(599..618)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1290..1384)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1405..2064)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1979..1998
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
       61 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
      121 gatgatttct ggaattcgcg gccgcttcta gaatctagag acgcatatgg gtctcatcga
      181 gacgaacaat aaggcctccc aaatcggggg gcctttttat ttttcaacaa aatgttagag
      241 accgcatgcc gtctcattag ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc
      301 cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac
      361 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      421 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      481 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct
      541 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      601 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      661 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      721 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      781 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      841 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      901 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
      961 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1021 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1081 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1141 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1201 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1261 taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa
     1321 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     1381 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg
     1441 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     1501 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     1561 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     1621 attggctgac acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     1681 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     1741 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     1801 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
     1861 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     1921 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     1981 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     2041 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     2101 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     2161 cc
//
