LOCUS       sequence-448201-        9181 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Non-integrating (episomal) expression of mouse p53DD - p53 
            carboxy-terminal dominant-negative fragment .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2012 Nov 29. doi: 10.1002/stem.1293."
COMMENT     Sequence Label: pCE-mp53DD
FEATURES             Location/Qualifiers
     source          1..9181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1794..1811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1965..2553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2045..2064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2724..3515,3516..3584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3347..3366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3585..3689)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        3720..4099
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          4396..5412
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5336..5358
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5420..5439
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(5877..5896)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5942..5997
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5996..6015)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6358..6374)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6358..6374)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6371..6393)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6382..6398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6406..6412,6413..6430,6431..6436))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6451..6472
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tatgctaccc agatataaat taggatagca tatactaccc agatatagat taggatagca
       61 tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat taggatagca
      121 tatgctatcc agatatttgg gtagtatatg ctacccatgg caacattagc ccaccgtgct
      181 ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc ttggcgctca ggcgcaagtg
      241 tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta tcttggcccg cccacctact
      301 tatgcaggta ttccccgggg tgccattagt ggttttgtgg gcaagtggtt tgaccgcagt
      361 ggttagcggg gttacaatca gccaagttat tacaccctta ttttacagtc caaaaccgca
      421 gggcggcgtg tgggggctga cgcgtgcccc cactccacaa tttcaaaaaa aagagtggcc
      481 acttgtcttt gtttatgggc cccattggcg tggagccccg tttaattttc gggggtgtta
      541 gagacaacca gtggagtccg ctgctgtcgg cgtccactct ctttcccctt gttacaaata
      601 gagtgtaaca acatggttca cctgtcttgg tccctgcctg ggacacatct taataacccc
      661 agtatcatat tgcactagga ttatgtgttg cccatagcca taaattcgtg tgagatggac
      721 atccagtctt tacggcttgt ccccacccca tggatttcta ttgttaaaga tattcagaat
      781 gtttcattcc tacactagta tttattgccc aaggggtttg tgagggttat attggtgtca
      841 tagcacaatg ccaccactga accccccgtc caaattttat tctgggggcg tcacctgaaa
      901 ccttgttttc gagcacctca catacacctt actgttcaca actcagcagt tattctatta
      961 gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg agagttcact gcccgctcct
     1021 tgatcttcag ccactgccct tgtgactaaa atggttcact accctcgtgg aatcctgacc
     1081 ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat atcgctgttc cttaggaccc
     1141 ttttactaac cctaattcga tagcatatgc ttcccgttgg gtaacatatg ctattgaatt
     1201 agggttagtc tggatagtat atactactac ccgggaagca tatgctaccc gtttagggtt
     1261 aacaaggggg ccttataaac actattgcta atgccctctt gagggtccgc ttatcggtag
     1321 ctacacaggc ccctctgatt gacgttggtg tagcctcccg tagtcttcct gggcccctgg
     1381 gaggtacatg tcccccagca ttggtgtaag agcttcagcc aagagttaca cataaaggca
     1441 atgttgtgtt gcagtccaca gactgcaaag tctgctccag gatgaaagcc actcagtgtt
     1501 ggcaaatgtg cacatccatt tataaggatg tcaactacag tcagagaacc cctttgtgtt
     1561 tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg gcaagttgta ccaaccaact
     1621 gaagggatta catgcactgc cccgcgaaga aggggcagag atgtcgtagt caggtttagt
     1681 tcgtccgggg cggggatcga tcctctagag tcgacgctag cggatccgct gcattaatga
     1741 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1801 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1861 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1921 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1981 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2041 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2101 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2161 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2221 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2281 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2341 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2401 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2461 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2521 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2581 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2641 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2701 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2761 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2821 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2881 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2941 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3001 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3061 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3121 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3181 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3241 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3301 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3361 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3421 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3481 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3541 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3601 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3661 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg
     3721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     3781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     3841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     3901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     3961 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4021 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     4081 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     4141 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     4201 gggggcgggg gggggggggg ggggggcccg gggggggggg gggggggggg gggggggggg
     4261 gggggggagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
     4321 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     4381 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
     4441 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     4501 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
     4561 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
     4621 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
     4681 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
     4741 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
     4801 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
     4861 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
     4921 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
     4981 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc
     5041 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
     5101 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
     5161 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
     5221 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag
     5281 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     5341 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
     5401 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
     5461 gaattcgccc ttcaccagga tgactgccat ggaggagtca cagtcggata tcagcctcaa
     5521 gagagcgctg cccacctgca caagcgcctc tcccccgcaa aagaaaaaac cacttgatgg
     5581 agagtatttc accctcaaga tccgcgggcg taaacgcttc gagatgttcc gggagctgaa
     5641 tgaggcctta gagttaaagg atgcccatgc tacagaggag tctggagaca gcagggctca
     5701 ctccagctac ctgaagacca agaagggcca gtctacttcc cgccataaaa aaacaatggt
     5761 caagaaagtg gggcctgact cagactgaag ggcgaattca ctcctcaggt gcaggctgcc
     5821 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     5881 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     5941 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     6001 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     6061 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     6121 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     6181 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     6241 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     6301 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     6361 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6421 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6481 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatct caattctcat
     6541 gtttgacagc ttatcatcga taagctgatc ctcacaggcc gcacccagct tttcttccgt
     6601 tgccccagta gcatctctgt ctggtgacct tgaagaggaa gaggaggggt cccgagaatc
     6661 cccatcccta ccgtccagca aaaaggggga cgaggaattt gaggcctggc ttgaggctca
     6721 ggacgcaaat cttgaggatg ttcagcggga gttttccggg ctgcgagtaa ttggtgatga
     6781 ggacgaggat ggttcggagg atggggaatt ttcagacctg gatctgtctg acagcgacca
     6841 tgaaggggat gagggtgggg gggctgttgg agggggcagg agtctgcact ccctgtattc
     6901 actgagcgtc gtctaataaa gatgtctatt gatctctttt agtgtgaatc atgtctgacg
     6961 aggggccagg tacaggacct ggaaatggcc taggagagaa gggagacaca tctggaccag
     7021 aaggctccgg cggcagtgga cctcaaagaa gagggggtga taaccatgga cgaggacggg
     7081 gaagaggacg aggacgagga ggcggaagac caggagcccc gggcggctca ggatcagggc
     7141 caagacatag agatggtgtc cggagacccc aaaaacgtcc aagttgcatt ggctgcaaag
     7201 ggacccacgg tggaacagga gcaggagcag gagcgggagg ggcaggagca ggaggggcag
     7261 gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagggg
     7321 caggagcagg aggaggggca ggagcaggag gggcaggagc aggaggtgga ggccggggtc
     7381 gaggaggcag tggaggccgg ggtcgaggag gtagtggagg ccggggtcga ggaggtagtg
     7441 gaggccgccg gggtagagga cgtgaaagag ccaggggggg aagtcgtgaa agagccaggg
     7501 ggagaggtcg tggacgtgga gaaaagaggc ccaggagtcc cagtagtcag tcatcatcat
     7561 ccgggtctcc accgcgcagg ccccctccag gtagaaggcc atttttccac cctgtagggg
     7621 aagccgatta ttttgaatac caccaagaag gtggcccaga tggtgagcct gacgtgcccc
     7681 cgggagcgat agagcagggc cccgcagatg acccaggaga aggcccaagc actggacccc
     7741 ggggtcaggg tgatggaggc aggcgcaaaa aaggagggtg gtttggaaag catcgtggtc
     7801 aaggaggttc caacccgaaa tttgagaaca ttgcagaagg tttaagagct ctcctggcta
     7861 ggagtcacgt agaaaggact accgacgaag gaacttgggt cgccggtgtg ttcgtatatg
     7921 gaggtagtaa gacctccctt tacaacctaa ggcgaggaac tgcccttgct attccacaat
     7981 gtcgtcttac accattgagt cgtctcccct ttggaatggc ccctggaccc ggcccacaac
     8041 ctggcccgct aagggagtcc attgtctgtt atttcatggt ctttttacaa actcatatat
     8101 ttgctgaggt tttgaaggat gcgattaagg accttgttat gacaaagccc gctcctacct
     8161 gcaatatcag ggtgactgtg tgcagctttg acgatggagt agatttgcct ccctggtttc
     8221 cacctatggt ggaaggggct gccgcggagg gtgatgacgg agatgacgga gatgaaggag
     8281 gtgatggaga tgagggtgag gaagggcagg agtgatgtaa cttgttagga gacgccctca
     8341 atcgtattaa aagccgtgta ttcccccgca ctaaagaata aatccccagt agacatcatg
     8401 cgtgctgttg gtgtatttct ggccatctgt cttgtcacca ttttcgtcct cccaacatgg
     8461 ggcaattggg catacccatg ttgtcacgtc actcagctcc gcgctcaaca ccttctcgcg
     8521 ttggaaaaca ttagcgacat ttacctggtg agcaatcaga catgcgacgg ctttagcctg
     8581 gcctccttaa attcacctaa gaatgggagc aaccagcagg aaaaggacaa gcagcgaaaa
     8641 ttcacgcccc cttgggaggt ggcggcatat gcaaaggata gcactcccac tctactactg
     8701 ggtatcatat gctgactgta tatgcatgag gatagcatat gctacccgga tacagattag
     8761 gatagcatat actacccaga tatagattag gatagcatat gctacccaga tatagattag
     8821 gatagcctat gctacccaga tataaattag gatagcatat actacccaga tatagattag
     8881 gatagcatat gctacccaga tatagattag gatagcctat gctacccaga tatagattag
     8941 gatagcatat gctacccaga tatagattag gatagcatat gctatccaga tatttgggta
     9001 gtatatgcta cccagatata aattaggata gcatatacta ccctaatctc tattaggata
     9061 gcatatgcta cccggataca gattaggata gcatatacta cccagatata gattaggata
     9121 gcatatgcta cccagatata gattaggata gcctatgcta cccagatata aattaggata
     9181 g
//
