LOCUS       sequence-448244-       12056 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express mEGFP-fusion protein of FMR1 fragment (1-491) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12056)
  AUTHORS   Chen X, Fansler MM, Janjos U, Ule J, Mayr C
  TITLE     The FXR1 network acts as signaling scaffold for actomyosin 
            remodeling.
  JOURNAL   Cell. 5 August 2024. doi: 10.1016/j.cell.2024.07.015.
  PUBMED    37961296
REFERENCE   2  (bases 1 to 12056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 5 
            August 2024. doi: 10.1016/j.cell.2024.07.015."
COMMENT     Sequence Label: pcDNA3puro-mGFP-FMR1iso1-N2(1-491)
FEATURES             Location/Qualifiers
     source          1..12056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        145..183
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     180..204
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      295..304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(301..303,304..306,307..1017)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(346..367)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(607..626)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     954..975
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2543..2569
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        2655..3866
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3703..3722
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(3925..4542,4543..4548,4549..4584,4585..4587,4588..4623,
                     4624..4626,4627..4662,4663..4671)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4704..5256
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4848..4865)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5075..5094
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5283..5882
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5283..5302)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5779..5799
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6056..6644
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6109..6129)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6527..6538)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6719..6952
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7071..7089)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7157..7261
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7262..7330,7331..8122)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7480..7499)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8293..8881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8782..8801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9035..9052
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9292..9313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9328..9333,9334..9351,9352..9358)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9366..9382
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9371..9393
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9390..9406
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9390..9406
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9456..9475
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9667..10300
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10347..10472
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10969..11202
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11387..11431
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11724..11841
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(11896..11914,11915..11937,11938..11956,11957..11979,
                     11980..11998,11999..12021,12022..12040,12041..12056,1..9,
                     10..28,29..51,52..70,71..93,94..112)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
       61 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
      121 agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt gggaggccta tataagcaga
      181 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      241 agaagacacc gggaccgatc cagcctccgc ggccccgaac tagtccagac gcgtgccacc
      301 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      361 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      421 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      481 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      541 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      601 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      661 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      721 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      781 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      841 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      901 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      961 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaaaaa
     1021 gcaggctcca ccatggagga gctggtggtg gaagtgcggg gctccaatgg cgctttctac
     1081 aaggcatttg taaaggatgt tcatgaagat tcaataacag ttgcatttga aaacaactgg
     1141 cagcctgata ggcagattcc atttcatgat gtcagattcc cacctcctgt aggttataat
     1201 aaagatataa atgaaagtga tgaagttgag gtgtattcca gagcaaatga aaaagagcct
     1261 tgctgttggt ggttagctaa agtgaggatg ataaagggtg agttttatgt gatagaatat
     1321 gcagcatgtg atgcaactta caatgaaatt gtcacaattg aacgtctaag atctgttaat
     1381 cccaacaaac ctgccacaaa agatactttc cataagatca agctggatgt gccagaagac
     1441 ttacggcaaa tgtgtgccaa agaggcggca cataaggatt ttaaaaaggc agttggtgcc
     1501 ttttctgtaa cttatgatcc agaaaattat cagcttgtca ttttgtccat caatgaagtc
     1561 acctcaaagc gagcacatat gctgattgac atgcactttc ggagtctgcg cactaagttg
     1621 tctctgataa tgagaaatga agaagctagt aagcagctgg agagttcaag gcagcttgcc
     1681 tcgagatttc atgaacagtt tatcgtaaga gaagatctga tgggtctagc tattggtact
     1741 catggtgcta atattcagca agctagaaaa gtacctgggg tcactgctat tgatctagat
     1801 gaagatacct gcacatttca tatttatgga gaggatcagg atgcagtgaa aaaagctaga
     1861 agctttctcg aatttgctga agatgtaata caagttccaa ggaacttagt aggcaaagta
     1921 ataggaaaaa atggaaagct gattcaggag attgtggaca agtcaggagt tgtgagggtg
     1981 aggattgagg ctgaaaatga gaaaaatgtt ccacaagaag aggaaattat gccaccaaat
     2041 tcccttcctt ccaataattc aagggttgga cctaatgccc cagaagaaaa aaaacattta
     2101 gatataaagg aaaacagcac ccatttttct caacctaaca gtacaaaagt ccagagggtg
     2161 ttagtggctt catcagttgt agcaggggaa tcccagaaac ctgaactcaa ggcttggcag
     2221 ggtatggtac catttgtttt tgtgggaaca aaggacagca tcgctaatgc cactgttctt
     2281 ttggattatc acctgaacta tttaaaggaa gtagaccagt tgagactcga gcggctacag
     2341 attgatgagc agttgcgaca gattggagct agttctagac caccaccaaa tcgtacagat
     2401 aaggaaaaaa gctatgtgac tgatgatggt caaggaatgg gtcgaggtag tagaccttac
     2461 agaaataggg ggcacggcag acgcggtcct ggatatactt caggataagc tagcctcgag
     2521 tctagagggc ccgcggttcg aatacccata cgacgtccca gactacgctt agtaatgatt
     2581 aattaaacta gaaattctac cgggtagggg aggcgctttt cccaaggcag tctggagtaa
     2641 cccacccaag atctggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct
     2701 cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg
     2761 gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca
     2821 gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag
     2881 cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg
     2941 cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca
     3001 gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag
     3061 cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg
     3121 tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg
     3181 ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt
     3241 gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa
     3301 ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac
     3361 catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt
     3421 gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc
     3481 gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc
     3541 tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt
     3601 aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc
     3661 agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa
     3721 ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt
     3781 aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct
     3841 ggccgttttt ggcttttttg ttagacgaag cttggtaccg agctcggatc tccaccccgt
     3901 accggtcctg cagtcgaatt caccatgtct agactggaca agagcaaagt cataaacgga
     3961 gctctggaat tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa
     4021 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
     4081 gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc
     4141 gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca
     4201 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
     4261 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
     4321 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa
     4381 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa
     4441 gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta
     4501 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac cgacgccctt
     4561 gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg
     4621 ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta actaagtaag
     4681 gatccgcggc cgcatcgata ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     4741 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     4801 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     4861 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     4921 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     4981 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     5041 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     5101 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     5161 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     5221 ggacgtggtt ttcctttgaa aaacacgatg ataatatgga tatggccaca acggtcgcca
     5281 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     5341 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     5401 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5461 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5521 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5581 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     5641 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     5701 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     5761 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     5821 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     5881 gacgcatgca tgcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     5941 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     6001 attgttgttg tttagtccct cccaattcga tatcaagctt atcgcgatag atcctaatca
     6061 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6121 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6181 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6241 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6301 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6361 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6421 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     6481 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6541 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6601 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga
     6661 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6721 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     6781 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6841 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6901 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     6961 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7021 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
     7081 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     7141 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7201 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7261 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7321 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7381 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7441 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7501 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7561 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7621 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7681 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7741 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7801 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7861 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7921 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7981 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8041 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8101 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8161 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8221 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8281 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8341 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8401 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8461 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8521 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8581 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8641 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8701 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8761 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8821 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8881 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8941 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9001 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9061 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa
     9121 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9181 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc
     9241 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     9301 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     9361 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt
     9421 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9481 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     9541 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
     9601 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
     9661 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct
     9721 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     9781 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     9841 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     9901 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     9961 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
    10021 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
    10081 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
    10141 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
    10201 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
    10261 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
    10321 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
    10381 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
    10441 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
    10501 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
    10561 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
    10621 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
    10681 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
    10741 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
    10801 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
    10861 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
    10921 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
    10981 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
    11041 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
    11101 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
    11161 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
    11221 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
    11281 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
    11341 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
    11401 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
    11461 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
    11521 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
    11581 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
    11641 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca
    11701 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
    11761 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
    11821 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggact
    11881 aggatccttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
    11941 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
    12001 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagt
//
