LOCUS       sequence-448393-        9141 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Stable expression and inducible recruitment of protein in mammalian 
            cell culture.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Adriaenssens E, Schaar S, Cook ASI, Stuke JFM, Sawa-Makarska J, 
            Nguyen TN, Ren X, Schuschnig M, Romanov J, Khuu G, Lazarou M, Hummer
            G, Hurley JH, Martens S
  TITLE     Reconstitution of BNIP3/NIX-mitophagy initiation reveals 
            hierarchical flexibility of the autophagy machinery
  JOURNAL   bioRxiv 2024.08.28.609967
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.08.28.609967"
COMMENT     Sequence Label: pHAGE-FKBP-mEGFP-WIPI2d (R108E/R125E)
FEATURES             Location/Qualifiers
     source          1..9141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2821..2830
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2827..2829,2830..3150)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             3157..3477
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             3157..3477
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(3496..3498,3499..4209)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3538..3559)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3799..3818)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4146..4167
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5531..6119
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5584..5604)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6002..6013)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6194..6427
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6546..6564)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6632..6736
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6737..6805,6806..7597)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6955..6974)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7768..8356
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8257..8276
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8510..8527
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8767..8788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8803..8808,8809..8826,8827..8833)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8841..8857
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8846..8868
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8865..8881
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8865..8881
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8931..8950
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcggtacc
     2821 gccaccatgg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag
     2881 cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat
     2941 tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga
     3001 ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct
     3061 ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc
     3121 gtcttcgatg tggagcttct aaaactggaa actagaggcg tgcaggtcga gaccatcagc
     3181 cccggcgacg gccgcacctt tcccaagaga ggccagactt gcgtggtcca ctacaccggc
     3241 atgctggagg acggcaagaa gttcgacagc agccgcgacc gcaacaagcc cttcaagttc
     3301 atgctgggca aacaggaagt gatccgcggc tgggaggaag gcgtggctca gatgagcgtg
     3361 gggcagcggg ccaagctgac catcagcccc gactatgcct acggcgccac cggccacccc
     3421 ggcatcatcc ccccccacgc caccctggtg ttcgacgtgg agctgctgaa gctggagact
     3481 agtaccggtt ctagagtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3541 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3601 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3661 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3721 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3781 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3841 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3901 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     3961 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4021 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4081 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     4141 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4201 ctgtacaagt ccggactcag atctcgagct caagcttcga attcgatgaa cctggcgagc
     4261 cagagcgggg aggccggcgc cggccagctg ctcttcgcca acttcaacca ggacaacacg
     4321 tccctagctg ttggtagtaa gtccggttat aaatttttct ccctttcttc tgtggataag
     4381 ctggaacaga tctatgaatg caccgatacg gaagatgtgt gcattgtaga gagattgttc
     4441 tccagcagcc tagtggccat cgtgagcctt aaagcaccaa ggaagctaaa ggtttgccac
     4501 tttaagaagg gaactgagat ctgcaactac agctactcca acacgattct ggctgtgaag
     4561 ctcaacgaac agaggctgat agtatgcctg gaggagtccc tgtacatcca caacattgaa
     4621 gacatgaagg tgctgcatac gatcagggag acgcctccaa accctgcagg cctgtgtgcg
     4681 ctgtcaatca acaacgacaa ctgctacttg gcgtacccag ggagcgcgac catcggagag
     4741 gtgcaggtct tcgataccat taatttgaga gctgcaaaca tgattccggc tcacgacagt
     4801 cctttagcgg cactggcctt tgacgcaagt ggaactaaac ttgccacggc ttcggagaag
     4861 gggaccgtga ttagggtatt ttccattcca gaaggacaaa aactctttga gtttcggaga
     4921 ggagtaaaga ggtgcgtgag catctgctcc ctggccttca gcatggacgg catgttcctc
     4981 tccgcctcca gcaacactga gaccgtgcac atcttcaaac tcgagactgt gaaagaaaaa
     5041 cccccagagg agcccaccac ctggaccggg tacttcggga aagtgctcat ggcctccacc
     5101 agctacctgc cttcccaagt gacagaaatg ttcaaccagg gcagagcctt cgccacggtc
     5161 cgcctgccat tctgcggcca caaaaacatc tgctcgctag ccacaattca gaagatcccg
     5221 cggttgttgg tgggtgccgc cgacgggtac ctgtacatgt acaacctgga cccccaggag
     5281 ggcggcgagt gtgccctgat gaagcagcac cggctggacg gcagtctgga aacgaccaat
     5341 gagatcttgg actctgcctc tcacgactgc cccttagtca ctcagacata cggcgcagct
     5401 gcaggaaaag cctacacaga cgacctgggt gctgtgggtg gcgcctgcct ggaggacgag
     5461 gccagcgccc tgcgcctgga tgaggacagc gagcacccgc ccatgattct tcggactgac
     5521 tgaggatcct aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5581 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5641 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5701 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5761 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5821 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5881 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     5941 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6001 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6061 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     6121 tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6181 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     6241 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6301 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6361 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6421 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     6481 atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg
     6541 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     6601 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6661 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6721 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6781 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6841 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6901 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6961 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7021 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7081 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7141 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7201 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7261 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7321 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7381 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7441 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7501 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7561 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7621 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7681 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7741 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7801 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7861 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7921 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7981 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8041 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8101 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8161 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8221 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8281 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8341 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8401 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8461 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8521 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     8581 tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8641 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8701 tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     8761 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     8821 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     8881 catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8941 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     9001 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     9061 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     9121 atgtactaca aacttagtag t
//
