LOCUS       sequence-448635-        8150 bp    DNA     circular SYN 14-APR-2025
DEFINITION  The pCDH-IFNA-neo plasmid is designed for the overexpression of 
            human IFN-alpha in mammalian cells. This plasmid was utilized to 
            engineer A549 cells for stable IFN-alpha overexpression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Min JY, Kim HM, Lee H, Cho MY, Park HS, Lee SY, Park MS, Ha SK, Kim 
            D, Jeong HG, Kim TD, Hong KS, Han EH
  TITLE     STAT1 as a tool for non-invasive monitoring of NK cell activation in
            cancer.
  JOURNAL   Commun Biol. 2024 Sep 30;7(1):1222. doi: 10.1038/s42003-024-06917-9.
  PUBMED    39349746
REFERENCE   2  (bases 1 to 8150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-09-30"; volume: "7(1):1222. doi"; pages: " 
            10.1038/s42003-024-06917-9"
COMMENT     Sequence Label: pCDH-IFNA-neo
FEATURES             Location/Qualifiers
     source          1..8150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(210..221)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             401..634
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    706..827
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(743..762)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     797..816
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      867..1002
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1035..1051)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1035..1051)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1048..1070)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1059..1075
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1083..1089,1090..1107,1108..1113))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1128..1149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1266..1283)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1437..2025)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(2196..2987,2988..3056))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2819..2838
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3057..3161)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3229..3247
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3285..3307)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3407..3426
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3620..3642
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3634..3651
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3635..3651
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3666..3892
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3893..4073
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4120..4245
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4738..4971
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5156..5200
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5467..5583
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5673..5876
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5827..5847
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5873..5897
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        6569..6780
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6793..7061
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             7088..7882
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7088..7882
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7142..7161)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7752..7771
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    join(7889..8150,1..327)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7942..7962)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
       61 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
      121 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
      181 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
      241 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
      301 ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca
      361 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
      421 caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
      661 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
      721 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      781 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
      841 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
      901 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
      961 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacc
     1021 aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     1081 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     1141 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     1201 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     1261 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1321 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1381 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1441 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1501 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1561 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1621 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1681 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1741 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1801 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1861 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1921 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1981 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2041 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2101 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2161 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2221 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     2281 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     2341 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     2401 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     2461 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     2521 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     2581 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     2641 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     2701 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     2761 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     2821 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     2881 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     2941 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     3001 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     3061 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     3121 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     3181 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     3241 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     3301 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     3361 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     3421 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     3481 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     3541 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     3601 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
     3661 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     3721 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     3781 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     3841 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     3901 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     3961 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     4021 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     4081 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     4141 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     4201 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     4261 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     4321 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     4381 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     4441 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     4501 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     4561 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
     4621 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     4681 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     4741 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     4801 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     4861 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     4921 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     4981 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     5041 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     5101 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     5161 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     5221 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     5281 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     5341 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     5401 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     5461 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     5521 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     5581 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     5641 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     5701 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     5761 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     5821 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     5881 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     5941 tctagagcta gcatggcctc gccctttgct ttactgatgg tcctggtggt gctcagctgc
     6001 aagtcaagct gctctctggg ctgtgatctc cctgagaccc acagcctgga taacaggagg
     6061 accttgatgc tcctggcaca aatgagcaga atctctcctt cctcctgtct gatggacaga
     6121 catgactttg gatttcccca ggaggagttt gatggcaacc agttccagaa ggctccagcc
     6181 atctctgtcc tccatgagct gatccagcag atcttcaacc tctttaccac aaaagattca
     6241 tctgctgctt gggatgagga cctcctagac aaattctgca ccgaactcta ccagcagctg
     6301 aatgacttgg aagcctgtgt gatgcaggag gagagggtgg gagaaactcc cctgatgaat
     6361 gcggactcca tcttggctgt gaagaaatac ttccgaagaa tcactctcta tctgacagag
     6421 aagaaataca gcccttgtgc ctgggaggtt gtcagagcag aaatcatgag atccctctct
     6481 ttatcaacaa acttgcaaga aagattaagg aggaaggaat aaatggatcg gccgcgaagg
     6541 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     6601 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     6661 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     6721 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     6781 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     6841 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     6901 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     6961 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     7021 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     7081 acgctagatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     7141 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     7201 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     7261 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     7321 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     7381 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     7441 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     7501 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     7561 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     7621 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     7681 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     7741 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     7801 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     7861 ccttcttgac gagttcttct gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     7921 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7981 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     8041 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     8101 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
//
