LOCUS       sequence-449598-        8956 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expressing METTL17-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Ast T, Itoh Y, Sadre S, McCoy JG, Namkoong G, Wengrod JC, Chicherin 
            I, Joshi PR, Kamenski P, Suess DLM, Amunts A, Mootha VK
  TITLE     METTL17 is an Fe-S cluster checkpoint for mitochondrial translation.
  JOURNAL   Mol Cell. 2024 Jan 18;84(2):359-374.e8. doi: 
            10.1016/j.molcel.2023.12.016. Epub 2024 Jan 9.
  PUBMED    38199006
REFERENCE   2  (bases 1 to 8956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.12.016"; journalName: "Mol Cell"; date: 
            "2024-01-18- 18"; volume: "84"; issue: "2"; pages: "359-374.e8"
COMMENT     Sequence Label: pLYS6-METTL17-FLAG
FEATURES             Location/Qualifiers
     source          1..8956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    470..595
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             642..822
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(823..1049)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1077..1097)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1077..1095)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1116..1132)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1116..1132)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1129..1151)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1140..1156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1164..1170,1171..1188,1189..1194))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1209..1230
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1347..1364)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1518..2106)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1598..1617)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2277..3068,3069..3137))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2900..2919
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3138..3242)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3268..3723)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3405..3426)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3617..3636
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3850..3872
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3864..3881
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3865..3881
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3891..3910
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3891..3909
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3930..4065
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4105..4226
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4116..4135)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4170..4189
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4298..4531
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4625..5428)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4736..4755)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5346..5365
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(5490..5509)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     misc_feature    6001..6118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6163..6228)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(7661..7864)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7690..7710)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(8153..8173)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(8471..8515)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8700..8933
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac
       61 tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa
      121 gatcagcggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt
      181 ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta
      241 ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc
      301 ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt
      361 ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc
      421 ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc
      481 acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc
      541 gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct
      601 ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac
      661 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
      721 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
      781 agagagctcc caggctcaga tctggtctaa ccagagagac ccgtttatgt atcgagctag
      841 gcacttaaat acaatatctc tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag
      901 acccgtctgt tgccttccta ataaggcacg atcgtaccac cttacttcca ccaatcggca
      961 tgcacggtgc tttttctctc cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca
     1021 agactacaag agtattgcat aagactacat taagcttgca gctccagctt ttgttccctt
     1081 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1141 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1201 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1261 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1321 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1381 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1441 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1501 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1561 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1621 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1681 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1741 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1801 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1861 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1921 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1981 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2041 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2101 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2161 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2221 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2281 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2341 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2401 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2461 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2521 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2581 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2641 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2701 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2761 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2821 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2881 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2941 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3001 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3061 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3121 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3181 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3241 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     3301 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3361 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3421 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3481 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3541 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3601 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3661 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     3721 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     3781 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     3841 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     3901 cactataggg cgaattgggt acgtccctag gcctccaaaa aagcctcctc actacttctg
     3961 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     4021 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     4081 cgggatagct agagccagac atgataagat acattgatga gtttggacaa accacaacta
     4141 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     4201 ccattataag ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt
     4261 tataaatact gaataataag atgacatgaa ctactactgc tagagatttt ccacactgac
     4321 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     4381 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     4441 agctcccagg ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct
     4501 tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta
     4561 agatctacag ctgccttgta agtcattggt cttaaagagc ttggctgcag gtcgacggat
     4621 cccctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     4681 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     4741 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     4801 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     4861 tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg
     4921 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     4981 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     5041 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     5101 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     5161 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     5221 gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     5281 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     5341 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     5401 atccatcttg ttcaatggcc gatcccatat tggctgcagg tcgaaaggcc cggagatgag
     5461 gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg
     5521 gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca
     5581 ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc
     5641 caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgaga
     5701 cgtgcggctt ccgtttgtca cgtccggcac gccgcgaacc gcaaggaacc ttcccgactt
     5761 aggggcggag caggaagcgt cgccgggggg cccacaaggg tagcggcgaa gatccgggtg
     5821 acgctgcgaa cggacgtgaa gaatgtgcga gacccagggt cggcgccgct gcgtttcccg
     5881 gaaccacgcc cagagcagcc gcgtccctgc gcaaacccag ggctgccttg gaaaaggcgc
     5941 aaccccaacc ccctcgagcc gcggccaaag tggatctctg ctgtccctgt aataaacccg
     6001 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
     6061 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt
     6121 gtggatgaat actgccattt gtctcgaggt cgagaattct cacttgtcgt cgtcgtcctt
     6181 ataatcgata tcatggtctt tgtagtcccc gtcgtggtcc ttgtaatcga actcaccgct
     6241 cccggcggct gaacccgcag atccgctctc tgacggatcc tgagcagtag aaggtggaaa
     6301 cgcgctgggc gtaagaaccg gaagcagatc tccccagctt gatacccggg cacaccgata
     6361 caggtcgcga ccgtgtcttc gcgccgtcaa cacggcgtgc tgcatatgtc cgtcgggaca
     6421 gcacaaatga caatgaacat gtcggggccg cttcaagact ggctgagtta tcctgggcca
     6481 tctgtgagct tcctcggggc tgccccgggc aagaatgacc atggagaatt tctcctcctt
     6541 gggcttcttg ttccatgaga agggaatagg atggtatgct tgagaaaatg aacaggccag
     6601 attcgtaagc tgcggacaag gaagttcatg cggacatggt gcaaaaacaa agccaggccg
     6661 agggtccagt ggagatttct ctttaccctt caatacaaga tctcgtgcat ccatgagaag
     6721 gctatggcct gcttttgtac cattctcgac aaggacgaga aaatgaccag ttttcctcca
     6781 caatgtttgc accacttccg tcctgtccgc ctttgatggc aactcgctaa gtgagaacgc
     6841 actaaccacg acgtcaaatt gtactttagg agatactgga aggaattggc ggaagaagac
     6901 tcctggaatg tatggttctc cactttctga gccgcctttc agcagctttt cggccaggac
     6961 cagcatggcc gcgcttcgat ctacacacat atactctcgc agactctgac cccaaatgga
     7021 gtgtgccgcc catgtaacac tgcccgtacc agatccgaag tccatcagag tctgaggctg
     7081 gaatgctgga ttgcgagcgc gaatctcgtg gaaagcgcgg gatactgcag caaagcctcc
     7141 gtcaagccgg gctgccatat aaacaaggga cagaccctcg gtatagctca gctcttgcca
     7201 gtgataggtt gtcttcctaa gcgcatggag aactgcgccg cgcaactttt cttccgtctg
     7261 actcaaatca gggttctcca agaacttttt ttcaaggtgc ctggcgcgtc tctgaagctc
     7321 ttcaggctca actgggagat gccttgacca aaggtagctg gtcaaggttt gcacttgatt
     7381 ttccattgta ggaccagcgc taccaagcag cagcaattgc gctccattcg cgagtgcctg
     7441 cgggagtcgg acgtgcggca gcttcaggat accgggatgt tgccggtggg gccttttctg
     7501 gagaaaccca gacttattgt cgacttgcgt gaccccagga accaaggctg cgagtgctcg
     7561 tgcctgtggt gcaactccca agccaggaca ccacctaccc agagtcaaca agcatttcaa
     7621 cgccgccgcc atgctagcgg atctgacggt tcactaaacc agctctgctt atatagacct
     7681 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
     7741 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
     7801 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
     7861 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
     7921 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     7981 gcgtacttgg catatgatag tccattttaa aacataattt taaaactgca aactacccaa
     8041 gaaattatta ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat
     8101 taattccaat tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg
     8161 gaaataggcc ctcggtgaag ggggcggccg ctcgaggcta gtctcgtgat cgataccgtc
     8221 gagatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta
     8281 ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg
     8341 aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta
     8401 ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa
     8461 accaattcca caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt
     8521 gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc
     8581 tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac
     8641 tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca
     8701 ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga
     8761 tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt
     8821 tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg
     8881 ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca
     8941 ctgctctttt ttctct
//
