LOCUS       sequence-449608-        9233 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Kawaguchi Y, Kovacs JJ, McLaurin A, Vance JM, Ito A, Yao TP
  TITLE     The deacetylase HDAC6 regulates aggresome formation and cell 
            viability in response to misfolded protein stress.
  JOURNAL   Cell. 2003 Dec 12. 115(6):727-38.
  PUBMED    14675537
REFERENCE   2  (bases 1 to 9233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2003 
            Dec 12. 115(6):727-38."
COMMENT     Sequence Label: pcDNA-HDAC6.Delta-Buz-FLAG
FEATURES             Location/Qualifiers
     source          1..9233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..30
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(149..169)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        154..483
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      334..469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     396..415
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             550..1344
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(604..623)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1214..1233
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1518..1639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1555..1574)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1609..1628
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1688..1704)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1688..1704)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1701..1723)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1712..1728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1736..1742,1743..1760,1761..1766))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1781..1802
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1919..1936)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2090..2678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2170..2189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2849..3640,3641..3709))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3472..3491
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3710..3814)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3889..3908)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4080..4459
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4460..4663
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4614..4634
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4708..4727
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4708..4726
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          4763..5335
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             8589..8612
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(8631..8649)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8632..8649)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8669..8686)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8675..8899
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(8945..9233,1..140)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9032..9051)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
       61 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      121 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
      181 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
      241 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      301 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
      361 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      421 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      481 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
      541 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
      601 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
      661 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
      721 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
      781 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
      841 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
      901 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
      961 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     1021 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     1081 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     1141 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     1201 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     1261 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     1321 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     1381 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     1441 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     1501 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1561 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     1621 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     1681 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     1741 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1801 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     1861 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     1921 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1981 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2041 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2101 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2161 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2221 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2281 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2341 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2401 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2461 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2521 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2581 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2641 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2701 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2761 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2821 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2881 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2941 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     3001 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3061 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3121 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3181 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3241 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3301 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3361 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3421 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3481 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3541 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3601 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3661 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3721 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3781 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3841 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     3901 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     3961 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     4021 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
     4081 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     4141 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4201 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4261 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     4321 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4381 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     4441 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     4501 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     4561 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     4621 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
     4681 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctag
     4741 agtcgatcct gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc
     4801 tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg
     4861 aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact
     4921 ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa
     4981 ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa
     5041 gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc
     5101 acagttttag agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa
     5161 ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacatcctgg tcatcatcct
     5221 gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa aatactctga
     5281 gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     5341 gggcaacgtg ctggtttgtg ctgtcgaccc caagcttggc ccgggcggat ccactagtaa
     5401 cggccgccag tgtgctggaa ttctgcagat atccatcaca ctggcggccg cccaccatga
     5461 cctcaacacg ccaggattcc accacaacca ggcagcgaag aagtaggcag aacccccagt
     5521 cgccccctca ggactccagt gtcacttcga agcgaaatat taaaaaggga gccgttcccc
     5581 gctctatccc caatctagcg gaggtaaaga agaaaggcaa aatgaagaag ctcggccaag
     5641 caatggaaga agacctaatc gtgggactgc aagggatgga tctgaacctt gaggctgaag
     5701 cactggctgg cactggcttg gtgttggatg agcagttaaa tgaattccat tgcctctggg
     5761 atgacagctt cccggaaggc cctgagcggc tccatgccat caaggagcaa ctgatccagg
     5821 agggcctcct agatcgctgc gtgtcctttc aggcccggtt tgctgaaaag gaagagctga
     5881 tgttggttca cagcctagaa tatattgatc tgatggaaac aacccagtac atgaatgagg
     5941 gagaactccg tgtcctagca gacacctacg actcagttta tctgcatccg aactcatact
     6001 cctgtgcctg cctggcctca ggctctgtcc tcaggctggt ggatgcggtc ctgggggctg
     6061 agatccggaa tggcatggcc atcattaggc ctcctggaca tcacgcccag cacagtctta
     6121 tggatggcta ttgcatgttc aaccacgtgg ctgtggcagc ccgctatgct caacagaaac
     6181 accgcatccg gagggtcctt atcgtagatt gggatgtgca ccacggtcaa ggaacacagt
     6241 tcaccttcga ccaggacccc agtgtcctct atttctccat ccaccgctac gagcagggta
     6301 ggttctggcc ccacctgaag gcctctaact ggtccaccac aggtttcggc caaggccaag
     6361 gatataccat caatgtgcct tggaaccagg tggggatgcg ggatgctgac tacattgctg
     6421 ctttcctgca cgtcctgctg ccagtcgccc tcgagttcca gcctcagctg gtcctggtgg
     6481 ctgctggatt tgatgccctg caaggggacc ccaagggtga gatggccgcc actccggcag
     6541 ggttcgccca gctaacccac ctgctcatgg gtctggcagg aggcaagctg atcctgtctc
     6601 tggagggtgg ctacaacctc cgcgccctgg ctgaaggcgt cagtgcttcg ctccacaccc
     6661 ttctgggaga cccttgcccc atgctggagt cacctggtgc cccctgccgg agtgcccagg
     6721 cttcagtttc ctgtgctctg gaagcccttg agcccttctg ggaggttctt gtgagatcaa
     6781 ctgagaccgt ggagagggac aacatggagg aggacaatgt agaggagagc gaggaggaag
     6841 gaccctggga gccccctgtg ctcccaatcc tgacatggcc agtgctacag tctcgcacag
     6901 ggctggtcta tgaccaaaat atgatgaatc actgcaactt gtgggacagc caccaccctg
     6961 aggtacccca gcgcatcttg cggatcatgt gccgtctgga ggagctgggc cttgccgggc
     7021 gctgcctcac cctgacaccg cgccctgcca cagaggctga gctgctcacc tgtcacagtg
     7081 ctgagtacgt gggtcatctc cgggccacag agaaaatgaa aacccgggag ctgcaccgtg
     7141 agagttccaa ctttgactcc atctatatct gccccagtac cttcgcctgt gcacagcttg
     7201 ccactggcgc tgcctgccgc ctggtggagg ctgtgctctc aggagaggtt ctgaatggtg
     7261 ctgctgtggt gcgtccccca ggacaccacg cagagcagga tgcagcttgc ggtttttgct
     7321 ttttcaactc tgtggctgtg gctgctcgcc atgcccagac tatcagtggg catgccctac
     7381 ggatcctgat tgtggattgg gatgtccacc acggtaatgg aactcagcac atgtttgagg
     7441 atgaccccag tgtgctatat gtgtccctgc accgctatga tcatggcacc ttcttcccca
     7501 tgggggatga gggtgccagc agccagatcg gccgggctgc gggcacaggc ttcaccgtca
     7561 acgtggcatg gaacgggccc cgcatgggtg atgctgacta cctagctgcc tggcatcgcc
     7621 tggtgcttcc cattgcctac gagtttaacc cagaactggt gctggtctca gctggctttg
     7681 atgctgcacg gggggatccg ctggggggct gccaggtgtc acctgagggt tatgcccacc
     7741 tcacccacct gctgatgggc cttgccagtg gccgcattat ccttatccta gagggtggct
     7801 ataacctgac atccatctca gagtccatgg ctgcctgcac tcgctccctc cttggagacc
     7861 caccacccct gctgaccctg ccacggcccc cactatcagg ggccctggcc tcaatcactg
     7921 agaccatcca agtccatcgc agatactggc gcagcttacg ggtcatgaag gtagaagaca
     7981 gagaaggacc ctccagttct aagttggtca ccaagaaggc accccaacca gccaaaccta
     8041 ggttagctga gcggatgacc acacgagaaa agaaggttct ggaagcaggc atggggaaag
     8101 tcacctcggc atcatttggg gaagagtcca ctccaggcca gactaactca gagacagctg
     8161 tggtggccct cactcaggac cagccctcag aggcagccac agggggagcc actctggccc
     8221 agaccatttc tgaggcagcc attgggggag ccatgctggg ccagaccacc tcagaggagg
     8281 ctgtcggggg agccactccg gaccagacca cctcagagga gactgtggga ggagccattc
     8341 tggaccagac cacctcagag gatgctgttg ggggagccac gctgggccag actacctcag
     8401 aggaggctgt aggaggagct acactggccc agaccatctc ggaggcagcc atggagggag
     8461 ccacactgga ccagactacg tcagaggagg ctccaggggg caccgagctg atccaaactc
     8521 ctctagcctc gagcacagac caccagaccc ccccaacctc acctgtgcag ggaactacac
     8581 cccagataga ttataaagat gatgatgata aataaactag agggccctat tctatagtgt
     8641 cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     8701 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     8761 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     8821 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     8881 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     8941 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     9001 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     9061 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     9121 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     9181 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gtt
//
