LOCUS       sequence-449795-        7810 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Transcription factor STAT5B with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7810)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 7810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-STAT5B-Brd 52
FEATURES             Location/Qualifiers
     source          1..7810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             14..247
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    319..440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(356..375)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     410..429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      480..615
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(635..654)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(636..654)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(664..681)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(664..680)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(673..695)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      822..1277
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(909..928)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1119..1140
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1303..1407
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1408..1476,1477..2268)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1626..1645)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2439..3027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2928..2947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3181..3198
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3315..3336
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3351..3356,3357..3374,3375..3381)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3389..3405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3394..3416
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3413..3429
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3413..3429
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3448..3468
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3450..3468
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3496..3722
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3723..3903
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3950..4075
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4568..4801
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4986..5030
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5297..5414
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5448..5854
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    7302..7705
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
ORIGIN
        1 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
       61 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      121 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      181 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
      241 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
      301 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      361 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
      421 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
      481 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
      541 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
      601 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
      661 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      721 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      781 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      841 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      901 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      961 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     1021 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     1081 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     1141 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     1201 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     1261 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1321 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1381 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1441 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1501 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1561 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1621 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     1681 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1741 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1801 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1861 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1921 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     1981 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2041 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2101 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     2161 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     2221 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     2281 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2341 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2401 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2461 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2521 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2581 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2641 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2701 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2761 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2821 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2881 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2941 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3001 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3061 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3121 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3181 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3241 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3301 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     3361 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     3421 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     3481 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     3541 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     3601 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     3661 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     3721 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3781 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3841 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3901 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     3961 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     4021 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     4081 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     4141 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     4201 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     4261 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     4321 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     4381 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     4441 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     4501 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     4561 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     4621 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     4681 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     4741 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     4801 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     4861 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     4921 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     4981 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     5041 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     5101 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     5161 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     5221 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     5281 gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     5341 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     5401 aaattcaaaa ttttatcgat cacgagacta gcctcgagct ggctgcagta acgccatttt
     5461 gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat
     5521 gaaaatagct aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc
     5581 ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg
     5641 tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc
     5701 ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg
     5761 cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg
     5821 gcgcgccagt cctccgacag actgagtcgg ccggtcgaat caagcttatc gataccgtcg
     5881 acggatccgc tagcccgcgg ttcgaaatgg ctgtgtggat acaagctcag cagctccaag
     5941 gagaagccct tcatcagatg caagcgttat atggccagca ttttcccatt gaggtgcggc
     6001 attatttatc ccagtggatt gaaagccaag catgggactc agtagatctt gataatccac
     6061 aggagaacat taaggccacc cagctcctgg agggcctggt gcaggagctg cagaagaagg
     6121 cagagcacca ggtgggggaa gatgggtttt tactgaagat caagctgggg cactatgcca
     6181 cacagctcca gaacacgtat gaccgctgcc ccatggagct ggtccgctgc atccgccata
     6241 tattgtacaa tgaacagagg ttggtccgag aagccaacaa tggtagctct ccagctggaa
     6301 gccttgctga tgccatgtcc cagaaacacc tccagatcaa ccagacgttt gaggagctgc
     6361 gactggtcac gcaggacaca gagaatgagt taaaaaagct gcagcagact caggagtact
     6421 tcatcatcca gtaccaggag agcctgagga tccaagctca gtttggcccg ctggcccagc
     6481 tgagccccca ggagcgtctg agccgggaga cggccctcca gcagaagcag gtgtctctgg
     6541 aggcctggtt gcagcgtgag gcacagacac tgcagcagta ccgcgtggag ctggccgaga
     6601 agcaccagaa gaccctgcag ctgctgcgga agcagcagac catcatcctg gatgacgagc
     6661 tgatccagtg gaagcggcgg cagcagctgg ccgggaacgg cgggcccccc gagggcagcc
     6721 tggacgtgct acagtcctgg tgtgagaagt tggccgagat catctggcag aaccggcagc
     6781 agatccgcag ggctgagcac ctctgccagc agctgcccat ccccggccca gtggaggaga
     6841 tgctggccga ggtcaacgcc accatcacgg acattatctc agccctggtg accagcacgt
     6901 tcatcattga gaagcagcct cctcaggtcc tgaagaccca gaccaagttt gcagccactg
     6961 tgcgcctgct ggtgggcggg aagctgaacg tgcacatgaa ccccccccag gtgaaggcca
     7021 ccatcatcag tgagcagcag gccaagtctc tgctcaagaa cgagaacacc cgcaagtaac
     7081 cggtcggatc ccccgggctg caggaattcg agcatcttac cgccatttat tcccatattt
     7141 gttctgtttt tcttgatttg ggtatacatt taaatgttaa taaaacaaaa tggtggggca
     7201 atcatttaca tttttaggga tatgtaatta ctagttcagg tgtattgcca caagacaaac
     7261 atgttaagaa actttcccgt tatttacgct ctgttcctgt taatcaacct ctggattaca
     7321 aaatttgtga aagattgact gatattctta actatgttgc tccttttacg ctgtgtggat
     7381 atgctgcttt aatgcctctg tatcatgcta ttgcttcccg tacggctttc gttttctcct
     7441 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtccgtcaac
     7501 gtggcgtggt gtgctctgtg tttgctgacg caacccccac tggctggggc attgccacca
     7561 cctgtcaact cctttctggg actttcgctt tccccctccc gatcgccacg gcagaactca
     7621 tcgccgcctg ccttgcccgc tgctggacag gggctaggtt gctgggcact gataattccg
     7681 tggtgttgtc ggggaagctg acgtcctttg caattcgata tcaagctgta ccttctagat
     7741 gtacactttt tcgagcgctt ctagaccaat gacttacaag gcagctgtag atcttagcca
     7801 ctttttaaaa
//
