LOCUS       sequence-449841-        8027 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Transcription factor NFIL3 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8027)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-NFIL3-Brd 54
FEATURES             Location/Qualifiers
     source          1..8027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..131
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             178..358
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(359..585)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(613..633)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(613..631)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(652..668)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(652..668)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(665..687)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    676..692
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(700..706,707..724,725..730))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    745..766
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(883..900)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1054..1642)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1134..1153)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1813..2604,2605..2673))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2436..2455
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2674..2778)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2804..3259)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2941..2962)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3153..3172
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3386..3408
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3400..3417
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3401..3417
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3427..3446
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3427..3445
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3466..3601
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3641..3762
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3652..3671)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3706..3725
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3834..4067
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4186..4589
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     promoter        complement(6254..6660)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    6694..6811
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7078..7122)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7307..7540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc
       61 gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg
      121 cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc
      181 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
      241 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
      301 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccgt
      361 ttatgtatcg agctaggcac ttaaatacaa tatctctgca atgcggcaat tcagtggttc
      421 gtccaatcca tgtcagaccc gtctgttgcc ttcctaataa ggcacgatcg taccacctta
      481 cttccaccaa tcggcatgca cggtgctttt tctctccttg taaggcatgt tgctaactca
      541 tcgttaccat gttgcaagac tacaagagta ttgcataaga ctacattaag cttgcagctc
      601 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
      661 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      721 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      781 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      841 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      901 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      961 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1021 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1081 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1141 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1201 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1261 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1321 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1381 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1441 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1501 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1561 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1621 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1681 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1741 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1801 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1861 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1921 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1981 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2041 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2101 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2161 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2221 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2281 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2341 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2401 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2461 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2521 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2581 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2641 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2701 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2761 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     2821 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     2881 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     2941 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     3001 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     3061 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     3121 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     3181 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     3241 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     3301 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     3361 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     3421 cgcgcgtaat acgactcact atagggcgaa ttgggtacgt ccctaggcct ccaaaaaagc
     3481 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     3541 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     3601 tgggcggagt taggggcggg atagctagag ccagacatga taagatacat tgatgagttt
     3661 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     3721 attgctttat ttgtaaccat tataagctgc aataaacaag ttcctctcac tctctgatat
     3781 tcatttcttt gcaagttata aatactgaat aataagatga catgaactac tactgctaga
     3841 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     3901 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     3961 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
     4021 agcaaaaagc agatcttgtc ttcgttggga gtgaattagc ccttccagtc cccccttttc
     4081 ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctag aagcgctatg
     4141 ttaaatgtac atctagaagg tacagcttga tatcgaattg caaaggacgt cagcttcccc
     4201 gacaacacca cggaattatc agtgcccagc aacctagccc ctgtccagca gcgggcaagg
     4261 caggcggcga tgagttctgc cgtggcgatc gggaggggga aagcgaaagt cccagaaagg
     4321 agttgacagg tggtggcaat gccccagcca gtgggggttg cgtcagcaaa cacagagcac
     4381 accacgccac gttgacggac aacgggccac aactcctcat aaagagacag caaccaggat
     4441 ttatacaagg aggagaaaac gaaagccgta cgggaagcaa tagcatgata cagaggcatt
     4501 aaagcagcat atccacacag cgtaaaagga gcaacatagt taagaatatc agtcaatctt
     4561 tcacaaattt tgtaatccag aggttgatta acaggaacag agcgtaaata acgggaaagt
     4621 ttcttaacat gtttgtcttg tggcaataca cctgaactag taattacata tccctaaaaa
     4681 tgtaaatgat tgccccacca ttttgtttta ttaacattta aatgtatacc caaatcaaga
     4741 aaaacagaac aaatatggga ataaatggcg gtaagatgct cgaattcctg cagcccgggg
     4801 gatccgaccg gtctacccag agtctgaagc agagattggt tgtgtggcta taagtctctt
     4861 gagtgagaca acctctgcag ataagtttgc tatcccctgc ttcaaataca agttctctgg
     4921 gtcagaaact ttgtagccac tgtctttcat ttcaacaact ccagttttga aactattctg
     4981 agttttgcca ctcagttctt tttgatgcca gtgctccgat ttgagagacc aatcttgaat
     5041 gttagtcact tgcactgaga aaggagtaag agaagaatgt accatacttg gggcactatg
     5101 cttttcgagt tcgaaatgtc ttttagatgt catgtcaata ggtgaggaaa gtttttgcgt
     5161 ggcctcaaat tcattatcaa aggcttctac tttgatctgc atggctttgg ctttgatccg
     5221 gagcttgtgt ggcaaggcag aggaattcac ttctggaact ttaaccacag ttgcatgcac
     5281 atgcttgagt tcaactggag aatggatggg gcccttgggg acctgttgct cgtcttctcc
     5341 atcagatgac tttcctacca caccatcatc agtttccgac gttctcgggg agttgctgga
     5401 ggatcggttg acttgcagta gtgggggaga gtgtgagtac ccagagaaag aattccccat
     5461 atagttttga tagatggacg ctgtgtaaga gcctcggtca tctcttggct cccttgtgta
     5521 gctctctaat tccatcggct cttgcttgat aatctggaac ttgttttcag gacttctgca
     5581 gcttccctgc acagagctct cctgcgtgtg ttctactgag gacacttctg aaacatcgga
     5641 cagcgagctt tgtggagagt gtttaatgac agaaatacaa ctacttgaca ccatcgaggg
     5701 ttcgtgctcg tccacaaatg aactcacatt ggatttggaa gtctggtaat cttgaaagta
     5761 cacagctgta gaattactga gtttctgaat ctcttgagca tatgctgtgg agctaattaa
     5821 accaaacttt aattttagtg aaagcagctc agcttttaaa gtggcgtttt cttctcccag
     5881 tgcaattagt ttgttctcta aaaccaggtc attcagtcga cgcttctcac gagatctttt
     5941 ggcagcttca ttatttttcc gccttttttc ccaatacata gcatctttct tttcatcagg
     6001 aatgaattcc cgtttcctcc gacatgcaga agatttgttc ttccccacac ttccttcact
     6061 gagaagcagc tcctcacctg ttgtggagtc ttctgacact tccgttaaag cagaattaag
     6121 gaccatcatc ttgtccacat tgctactggc atcaagagac gcctgctcct ttttgacggt
     6181 ctgcattttt ctcagctgca tttcgaaccg cgggctagcg gatccgtcga cggtatcgat
     6241 aagcttgatt cgaccggccg actcagtctg tcggaggact ggcgcgccga gtgaggggtt
     6301 gtgagctctt ttatagagct cgggaagcag aagcgcgcga acagaagcga gaagcaggct
     6361 gattggttaa ttcaaataag gcgcagggtc atttcaggtc cttgggggag cctggaaaca
     6421 tctgatgggt cttaagaaac tgctgagggt tgggccatat ctggggacca tctgttcttg
     6481 gcctcgggcc ggggccgaaa ctgcggtgac catctgttct tggccccggg ccggggccga
     6541 aactgctcac cgcagatatc ctgtttggcc caacgttagc tattttcatg tacccgccct
     6601 tgatctgaac ttctctattc ttggtttggt atttttccat gccttgcaaa atggcgttac
     6661 tgcagccagc tcgaggctag tctcgtgatc gataaaattt tgaatttttg taatttgttt
     6721 ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact
     6781 gtacccccca atcccccctt ttcttttaaa agttaaccga taccgtcgag atccgttcac
     6841 taatcgaatg gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct
     6901 gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct
     6961 aactctattc actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac
     7021 tatcattatg aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa
     7081 acttgcccat ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga
     7141 ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat
     7201 ccaggtcgtg tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg
     7261 cacagcagtg gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc
     7321 ctttaggtat ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt
     7381 gagttgcaac agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac
     7441 tataccagac aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt
     7501 gcttcctgct gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc
     7561 tctctgcacc actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac
     7621 tactttatat ttatataatt cacttctcca attgtccctc atatctcctc ctccaggtct
     7681 gaagatcagc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct
     7741 tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg
     7801 ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct
     7861 gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc
     7921 gttctagctc cctgcttgcc catactatat gttttaattt atattttttc tttccccctg
     7981 gccttaaccg aattttttcc catcgcgatc taattctccc ccgctta
//
