LOCUS       sequence-449880-        7613 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Transcription factor PAX5 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7613)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 7613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-PAX5-Brd 62
FEATURES             Location/Qualifiers
     source          1..7613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    221..342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(258..277)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     312..331
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      382..517
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(537..556)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(538..556)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(566..583)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(566..582)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(575..597)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      724..1179
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(811..830)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1021..1042
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1205..1309
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1310..1378,1379..2170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1528..1547)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2341..2929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2830..2849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3083..3100
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3217..3238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3253..3258,3259..3276,3277..3283)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3291..3307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3296..3318
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3315..3331
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3315..3331
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3350..3370
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3352..3370
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3398..3624
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3625..3805
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3852..3977
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4470..4703
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4888..4932
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5199..5316
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5350..5756
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    7007..7410
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             join(7529..7613,1..149)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
       61 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
      121 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
      181 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
      241 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      301 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
      361 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      421 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      481 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
      541 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      601 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      661 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      721 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      781 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      841 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      901 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      961 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     1021 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     1081 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     1141 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     1201 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1261 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     1321 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     1381 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     1441 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     1501 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     1561 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     1621 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     1681 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     1741 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1801 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1861 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1921 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1981 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2041 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2101 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2161 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2221 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2281 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2341 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2401 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2461 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2521 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2581 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2641 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2701 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2761 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2821 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2881 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2941 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3001 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3061 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     3121 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     3181 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     3241 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     3301 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     3361 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     3421 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     3481 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     3541 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     3601 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     3661 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3721 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3781 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     3841 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     3901 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     3961 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     4021 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     4081 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     4141 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     4201 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     4261 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     4321 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     4381 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     4441 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     4501 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     4561 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     4621 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     4681 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     4741 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     4801 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     4861 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     4921 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     4981 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     5041 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     5101 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     5161 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     5221 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     5281 gaattacaaa aacaaattac aaaaattcaa aattttatcg atcacgagac tagcctcgag
     5341 ctggctgcag taacgccatt ttgcaaggca tggaaaaata ccaaaccaag aatagagaag
     5401 ttcagatcaa gggcgggtac atgaaaatag ctaacgttgg gccaaacagg atatctgcgg
     5461 tgagcagttt cggccccggc ccggggccaa gaacagatgg tcaccgcagt ttcggccccg
     5521 gcccgaggcc aagaacagat ggtccccaga tatggcccaa ccctcagcag tttcttaaga
     5581 cccatcagat gtttccaggc tcccccaagg acctgaaatg accctgcgcc ttatttgaat
     5641 taaccaatca gcctgcttct cgcttctgtt cgcgcgcttc tgcttcccga gctctataaa
     5701 agagctcaca acccctcact cggcgcgcca gtcctccgac agactgagtc ggccggtcga
     5761 atcaagctta tcgataccgt cgacggatcc gctagcccgc ggttcgaaat ggatttagag
     5821 aaaaattatc cgactcctcg gaccagcagg acaggacatg gaggagtgaa tcagcttggg
     5881 ggggtttttg tgaatggacg gccactcccg gatgtagtcc gccagaggat agtggaactt
     5941 gctcatcaag gtgtcaggcc ctgcgacatc tccaggcagc ttcgggtcag ccatggttgt
     6001 gtcagcaaaa ttcttggcag gtattatgag acaggaagca tcaagcctgg ggtaattgga
     6061 ggatccaaac caaaggtcgc cacacccaaa gtggtggaaa aaatcgctga atataaacgc
     6121 caaaatccca ccatgtttgc ctgggagatc agggaccggc tgctggcaga gcgggtgtgt
     6181 gacaatgaca ccgtgcctag cgtcagttcc atcaacagga tcatccggac aaaagtacag
     6241 cagccaccca accaaccagt cccagcttcc agtcacagca tagtgtccac tggctccgtg
     6301 acgcaggtgt cctcggtgag cacggattcg gccggctcgt cgtactccat cagcggcatc
     6361 ctgggcatca cgtcccccag cgccgacacc aacaagcgca agagagacga aggtattcag
     6421 gagtctccgg tgccgaacgg ccactcgctt ccgggcagag acttcctccg gaagcagatg
     6481 cggggagact tgttcacaca gcagcagctg gaggtgctgg accgcgtgtt tgagaggcag
     6541 cactactcag acatcttcac caccacagag cccatcaagc ccgagcaggg agtgagtttt
     6601 ccgggagtcc ctacagccac cctcagtatt cctcgtacaa cgactcctgg aggttcccca
     6661 acccggggct gcttggctcc ccctactatt atagcgctgc cgcccgagga gccgccccac
     6721 ctgcagccgc cactgcctat gaccgtcact gacccttgga gccaggcggg caccaaacac
     6781 tagaccggtc ggatcccccg ggctgcagga attcgagcat cttaccgcca tttattccca
     6841 tatttgttct gtttttcttg atttgggtat acatttaaat gttaataaaa caaaatggtg
     6901 gggcaatcat ttacattttt agggatatgt aattactagt tcaggtgtat tgccacaaga
     6961 caaacatgtt aagaaacttt cccgttattt acgctctgtt cctgttaatc aacctctgga
     7021 ttacaaaatt tgtgaaagat tgactgatat tcttaactat gttgctcctt ttacgctgtg
     7081 tggatatgct gctttaatgc ctctgtatca tgctattgct tcccgtacgg ctttcgtttt
     7141 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtccg
     7201 tcaacgtggc gtggtgtgct ctgtgtttgc tgacgcaacc cccactggct ggggcattgc
     7261 caccacctgt caactccttt ctgggacttt cgctttcccc ctcccgatcg ccacggcaga
     7321 actcatcgcc gcctgccttg cccgctgctg gacaggggct aggttgctgg gcactgataa
     7381 ttccgtggtg ttgtcgggga agctgacgtc ctttgcaatt cgatatcaag ctgtaccttc
     7441 tagatgtaca ataactcgag cgcttctaga ccaatgactt acaaggcagc tgtagatctt
     7501 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     7561 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctg
//
