LOCUS       sequence-449884-        9200 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Transcription factor TRPS1 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9200)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 9200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-TRPS1-Brd 67
FEATURES             Location/Qualifiers
     source          1..9200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    592..717
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             764..944
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(945..1171)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1199..1219)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1199..1217)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1238..1254)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1238..1254)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1251..1273)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1262..1278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1286..1292,1293..1310,1311..1316))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1331..1352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1469..1486)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1640..2228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1720..1739)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2399..3190,3191..3259))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3022..3041
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3260..3364)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3390..3845)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3527..3548)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3739..3758
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3972..3994
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3986..4003
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3987..4003
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4013..4032
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4013..4031
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4052..4187
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4227..4348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4238..4257)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4292..4311
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4420..4653
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4772..5175
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     promoter        complement(8013..8419)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    8453..8570
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8837..8881)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9066..9200,1..99)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
       61 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
      121 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
      181 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg
      241 aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt
      301 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
      361 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
      421 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg
      481 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
      541 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
      601 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
      661 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
      721 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
      781 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
      841 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
      901 ccagagagct cccaggctca gatctggtct aaccagagag acccgtttat gtatcgagct
      961 aggcacttaa atacaatatc tctgcaatgc ggcaattcag tggttcgtcc aatccatgtc
     1021 agacccgtct gttgccttcc taataaggca cgatcgtacc accttacttc caccaatcgg
     1081 catgcacggt gctttttctc tccttgtaag gcatgttgct aactcatcgt taccatgttg
     1141 caagactaca agagtattgc ataagactac attaagcttg cagctccagc ttttgttccc
     1201 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     1261 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1321 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1381 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1441 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1501 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1561 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1621 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1681 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1741 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1801 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1861 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1921 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1981 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2041 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2101 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2161 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2221 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2281 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2341 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2401 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2461 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2521 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2581 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2641 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2701 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2761 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2821 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2881 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2941 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3001 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3061 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3121 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3181 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3361 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     3421 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     3481 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     3541 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     3601 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     3661 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     3721 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     3781 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     3841 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     3901 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     3961 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     4021 ctcactatag ggcgaattgg gtacgtccct aggcctccaa aaaagcctcc tcactacttc
     4081 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     4141 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     4201 ggcgggatag ctagagccag acatgataag atacattgat gagtttggac aaaccacaac
     4261 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     4321 aaccattata agctgcaata aacaagttcc tctcactctc tgatattcat ttctttgcaa
     4381 gttataaata ctgaataata agatgacatg aactactact gctagagatt ttccacactg
     4441 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     4501 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     4561 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat
     4621 cttgtcttcg ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc
     4681 taagatctac agctgccttg taagtcattg gtctagaagc gctgaaggac ctgtacatct
     4741 agaaggtaca gcttgatatc gaattgcaaa ggacgtcagc ttccccgaca acaccacgga
     4801 attatcagtg cccagcaacc tagcccctgt ccagcagcgg gcaaggcagg cggcgatgag
     4861 ttctgccgtg gcgatcggga gggggaaagc gaaagtccca gaaaggagtt gacaggtggt
     4921 ggcaatgccc cagccagtgg gggttgcgtc agcaaacaca gagcacacca cgccacgttg
     4981 acggacaacg ggccacaact cctcataaag agacagcaac caggatttat acaaggagga
     5041 gaaaacgaaa gccgtacggg aagcaatagc atgatacaga ggcattaaag cagcatatcc
     5101 acacagcgta aaaggagcaa catagttaag aatatcagtc aatctttcac aaattttgta
     5161 atccagaggt tgattaacag gaacagagcg taaataacgg gaaagtttct taacatgttt
     5221 gtcttgtggc aatacacctg aactagtaat tacatatccc taaaaatgta aatgattgcc
     5281 ccaccatttt gttttattaa catttaaatg tatacccaaa tcaagaaaaa cagaacaaat
     5341 atgggaataa atggcggtaa gatgctcgaa ttcctgcagc ccgggggatc cgaccggtct
     5401 acaggaatcc cttggtttcc acagccaagc cataaatagg tcgcgccaga tgggcggcct
     5461 ccacattggg actatccctt agagtctttg tctgctcttg ggtgccagac acaggcgtca
     5521 gcagccccag gcttgcttgg gtgtatgacg gactcccccg caggatgtct gcccctctcc
     5581 aagtcacatt gcgaaggtca tcactggaac tctcggtcca aactttctct ttgagcccgt
     5641 ccttctcttc cagcttctct ctcttcacca cactctcaga aactggctct cccattttag
     5701 agtctggagt tagcagattg tagaccctga agtcaatttt gggctcctct ttgatggtgg
     5761 atatggcatg accgtcctct tcgccgttgg ctgtagtgat gtcctgttcc tggcagtgaa
     5821 cagtgttgaa gtgctccagt agtgactgag tatcggcagc tgtaaaactg cactgacggc
     5881 atttgtagca gctgtgtgct ctcctgtagt gtcgggaaat ctcttcttcc acttgggtaa
     5941 taaaatcaca tttggtacat gagtgttctt tgctttcctt gacagactgc tgcccatccg
     6001 atccttgcag gtgatttgct tcttgtttga catccgatgc ttgggactca tgcacacttt
     6061 catagtgaaa gaggagtaca tctacgtcag gggtggtgaa tgaacactga tggcactgat
     6121 gtttgactcg cgagcttcca gccgccccag gagacaagtg cagaagcaag agtgcacagt
     6181 gggaacaatt actttttcta caagcaaacg gataagtaat ttctccaagg tgcttttctg
     6241 ggctgcaaag tcctctggga cagaatggac agtgtttaat ggtacacttg tgaatgttat
     6301 ggagctgttg ataatgacgg agaagtggcc ccactacaat tacatcaggg ccatggcttt
     6361 tggaatatcg gaagtcacag aactgacaat tatagctcgt taccatatta tcctcggctc
     6421 ccttgctgga gaagtccttc tttttagccc cactcgagct cttgtctgtc ttggtcattg
     6481 tctctccttc tgaacttttg gctagatcat tctgattaat gacagagccc ctggaaagct
     6541 tatcatttaa ctctggatta aggccgcctg actgcactgc tccgtgctgc ttgccataat
     6601 gttctagcag tttaagtgag ctagatgact cacagctgaa actacaaaat ttacaccagt
     6661 agtaactggt ggcctctgta ccattttgtc tagaggaatc gaggggcttt atgggcactg
     6721 agtacccaac aggagtgtca tctcctgctt tgactgttat cttgtcctgc cattttccca
     6781 agtctccaga atcactggat tgaagtgcag ggatggactt gttagagttt ttctctgaag
     6841 gttttgcaac ctcagaggag gggagagaag cttttatttt gtttgggtga gtctgaagaa
     6901 aatgttgttc taattcggtg gatgaattgc ccatataagt gaaattgcag aatttacagc
     6961 ggaaatactt ggtgttccct tggcaatctg gtgttttccg tccaatgcca atgaatgttc
     7021 cacctgaagt cacctgcaca tcgtaggtcc catttagtaa aacaggcctt gaagaattga
     7081 tgtcctgcag cacaccagaa aacacagaac ggttgacctt ctggaagtct ttggaatggc
     7141 tgaactgcac catgttatga agggccaaga ttttgctgtc cagctcagca tcttgcctgg
     7201 tgcggttatg cagtcctaag tgatactttc ggaagtgctt aatcagatct gtggggtcgt
     7261 tgccgtagta accatatcca cagatattgc atttaaagtc ctgaagctct ggagacagag
     7321 gtgccgggtc tgggttgtca ttcaccagta agtcagtttt ggatttattc agtcttacac
     7381 ccccatctga aggcacttgt gggttttttg aggccactga aactgggctc aaaccttgac
     7441 aattggcttg accactctgt gcttgccctg tttcctctgt agcctttggt gacatcttct
     7501 gatcttcctt tgtctccagt gagtcccctg agggggtgca ggccatatct tgagggtcat
     7561 ctgcctctgc tctttgcgga gacttcaagg gctcacagac tcccccagca gctggagatg
     7621 agaaagccaa catatttctg tctgtcacct catcatgcgg aaaggaggga aagtttcctc
     7681 ccttactggg gctttcataa ttgaagccag ccttctcact cagaactgcg cttttcaagt
     7741 ccttcttact gctagaagat ggatcttgaa catgcaagct atgttcctcc ttatgattta
     7801 gttctgcagc atcactctga tccgtatttt ctgacatctg atctgcagaa aattctttgt
     7861 tctttccaga taccttgctt tctgtaccta taggctccag gatctggccc tcgccttcac
     7921 ttgcaacgtt tctcagaggg gggttctttt tccggaccat ttcgaaccgc gggctagcgg
     7981 atccgtcgac ggtatcgata agcttgattc gaccggccga ctcagtctgt cggaggactg
     8041 gcgcgccgag tgaggggttg tgagctcttt tatagagctc gggaagcaga agcgcgcgaa
     8101 cagaagcgag aagcaggctg attggttaat tcaaataagg cgcagggtca tttcaggtcc
     8161 ttgggggagc ctggaaacat ctgatgggtc ttaagaaact gctgagggtt gggccatatc
     8221 tggggaccat ctgttcttgg cctcgggccg gggccgaaac tgcggtgacc atctgttctt
     8281 ggccccgggc cggggccgaa actgctcacc gcagatatcc tgtttggccc aacgttagct
     8341 attttcatgt acccgccctt gatctgaact tctctattct tggtttggta tttttccatg
     8401 ccttgcaaaa tggcgttact gcagccagct cgaggctagt ctcgtgatcg ataaaatttt
     8461 gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta
     8521 ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa gttaaccgat
     8581 accgtcgaga tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt
     8641 cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata
     8701 atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa actattctta
     8761 aacctaccaa gcctcctact atcattatga ataattttat ataccacagc caatttgtta
     8821 tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct tgttcattct
     8881 tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa
     8941 tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac
     9001 tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa
     9061 tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct
     9121 gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca
     9181 aattgttctg ctgctgcact
//
