LOCUS       sequence-449904-        9923 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of rTetR(SE) fused to EED and mTurquoise-NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Sinha J, Nickels JF, Thurm AR, Ludwig CH, Archibald BN, Hinks MM, 
            Wan J, Fang D, Bintu L
  TITLE     The H3.3 K36M oncohistone disrupts the establishment of epigenetic 
            memory through loss of DNA methylation.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147.
  PUBMED    37873347
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147."
COMMENT     Sequence Label: pLenti-rTetR(SE)-EED-2A-mTurquoise
FEATURES             Location/Qualifiers
     source          1..9923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             651..831
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1340..1356
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1341..1357
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1360..1948
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1466..1477
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1875..1895
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    2004..2037
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2060..2080)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(2099..2809,2810..2812,2813..2815))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2141..2162)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2490..2509
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2749..2770
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2816..2869)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(4220..4285)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          4926..5864
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4949..4969)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(5865..6076)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    6199..6316
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6614..6658)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6843..7076
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7573..7698
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7745..7925
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7943..8141)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7968..7988)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8143..8522
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8694..8713
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8788..8892
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8893..8961,8962..9753)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9111..9130)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat
      661 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
      721 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      781 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
      841 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
      901 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
      961 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     1021 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     1081 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     1141 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     1201 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     1261 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     1321 atgtttttct aggtctcgat cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg
     1381 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     1441 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     1501 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     1561 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     1621 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     1681 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     1741 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     1801 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     1861 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     1921 cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt
     1981 ggaaccggaa cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag
     2041 gtccctcgac gaattcttat acctttctct tcttttttgg gctaccgctg ccgctaccct
     2101 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     2161 tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctgggtgctc aggtagtggt
     2221 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     2281 gctgcacgcc gccgtcctcg atgttgtggc ggatcttgaa gttggccttg atgccgttct
     2341 tctgcttgtc ggcggtgata tagacgttgt cgctgatgta gttgtactcc agcttgtgcc
     2401 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     2461 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     2521 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     2581 tgtggtcggg gtagcgggcg aagcactgca cgccccagga cagggtggtc acgagggtgg
     2641 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     2701 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     2761 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accattgggc
     2821 caggattctc ctcgacatct ccgcaagtta ggaggcttcc ccttccctct ccagatcctc
     2881 gaagtcgatc ccagcgccaa atgctggcat catcgcagac agctatgagg atgctgctat
     2941 ccctactgaa actggtttgt cgaatagccg cgccacattt atgatgggtc agtgttgtgc
     3001 atttggcttt atgaggatct tctacttcta aatcccaaac atacagtttg ccaacctgat
     3061 tgcccaatgc aagcatcttt tgccagaaat ccatagaaaa cctcatgtac caaatgtcac
     3121 actggctgta atcaaatcgc ccaagaatag tcacattaga ctcactaggt ttaattttat
     3181 ctatatcatc ctccattttg ccaggtttcc agcatacaat ggcattttca caagacttgg
     3241 aaagtatcaa atcgcctaac catcgcacac aatcaacata attcctatgt atgtctctgg
     3301 tagaaaagtc aggaaagtgg attttctggg aaataaatgg cctgttagtt ttgtttgggt
     3361 tataatcata agactcctta attgcattca tcatcctctt tgagttgatt ctccacagtt
     3421 taagagagtg atccatacca caggacatta ttttttcacc caaaagatca taatcagcac
     3481 tcagaacttc atctctgtgc ccttccacac ctccgaatat tgccacaaga gtgtctgttt
     3541 ggatattcca taaccgtaaa gcatgatctt tacttactga caggagaagg tttgggtctc
     3601 gtgggtggaa tttcagctca ttgatagcat ttccatggcc aacatagtgc tttatacact
     3661 gcattgttat aggattaatt attcttataa tgcctctaga tccagcaact gctaatagag
     3721 ggtggctggt gttgctatca taggtccatg cacaagtgta aaagttttca tctgcatcag
     3781 catctacata ggactgcaat aaccgtatct ccccctgtga atggcattcg tataaggtta
     3841 ctctgttgct tcccacagtt gcaaacacca gagggtctcc ttctttactg tgccagttaa
     3901 actgaactcc aaacaatggc tggttatgat cttccttgag gctgttcaca catttgaaag
     3961 aatatttgca tttctttgac ttccattttc cctttcccca gcttttcctt cctggtgcat
     4021 ttggcgtatt tgtgggcgtg tccgggcgtt ctgtgtttgt gccactctca atactgacag
     4081 catcgtcatt ttcgtctccc gagaggtccg ggttgctgtt ctcgtcgctg ctcaacttct
     4141 gcttcttggc cgcgggcatg tctgttcccg ccggcgcagt cgacacttcc ctctcggagg
     4201 agcctgatcc ggatccaccc ttgtcatcgt catccttgta gtcgatgtca tgatctttat
     4261 aatcaccgtc atggtctttg tagtcaccgc cgctttcgca ctttagctgt ttctccaggc
     4321 cacatatgat tagttccagg ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca
     4381 gctcaattgc ttgtttcaga agtgggggca tagaatcggt ggtaggtgtc tctctttcct
     4441 cttttgctac ttgatgctcc tgttcctcca atacgcagcc cagtgtaaag tggcccacgg
     4501 cggacagagc gtacagtgcg ttctccaggg agaagccttg ctgacacagg aacgcgagct
     4561 gattttccag ggtttcgtac tgtttctctg ttgggcgggt gccgagatgc actttagccc
     4621 cgtcgcgatg tgagaggaga gcacagcggt atgacttggc gttgttccgc agaaagtctt
     4681 gccatgactc gggttccagg gggcaggagt gggtatgatg cctgtccagc atctcgattg
     4741 gcagggcatc gagcagggcc cgcttgttct tcacgtgcca gtacagggta ggctgctcaa
     4801 ctcccagctt ttgagcgagt ttccttgtcg tcaggccttc gataccgaca ccattgagta
     4861 attccagagc tccgtttatt attttgctct tgtccagtct agacatggtg gctagctcac
     4921 gacacctgaa atggaagaaa aaaactttga accactgtct gaggcttgag aatgaaccaa
     4981 gatccaaact caaaaagggc aaattccaag gagaattaca tcaagtgcca agctggccta
     5041 acttcagtct ccacccactc agtgtgggga aactccatcg cataaaaccc ctccccccaa
     5101 cctaaagacg acgtactcca aaagcgcgag aactaatcga ggtgcctgga cggcgcccgg
     5161 tactccgtgg agtcacatga agcgacggct gaggacggaa aggccctttt cctttgtgtg
     5221 ggtgactcac ccgcccgctc tcccgagcgc cgcgtcctcc attttgagct ccctgcagca
     5281 gggccgggaa gcggccatct ttccgctcac gcaactggtg ccgaccgggc cagccttgcc
     5341 gcccagggcg gggcgataca cggcggcgcg aggccaggca ccagagcagg ccggccagct
     5401 tgagactacc cccgtccgat tctcggtggc cgcgctcgca ggccccgcct cgccgaacat
     5461 gtgcgctggg acgcacgggc cccgtcgccg cccgcggccc caaaaaccga aataccagtg
     5521 tgcagatctt ggcccgcatt tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt
     5581 catcaaaaat tttaaatggc tagagactta tcgaaagcag cgagacaggc gcgaaggtgc
     5641 caccagattc gcacgcggcg gccccagcgc ccaggccagg cctcaactca agcacgaggc
     5701 gaaggggctc cttaagcgca aggcctcgaa ctctcccacc cacttccaac ccgaagctcg
     5761 ggatcaagaa tcacgtactg cagccaggtg gaagtaattc aaggcacgca agggccataa
     5821 cccgtaaaga ggccaggccc gcgggaacca cacacggcac ttacctgtgt tctggcggca
     5881 aacccgttgc gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca
     5941 ccctcgggaa aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac
     6001 cttctctagg caccggttca attgccgacc cctcccccca acttctcggg gactgtgggc
     6061 gatgtgcgct ctgcccactg acgggcacat gcatggcggt aatacggtta tccacgcggc
     6121 cgcctgagtc aaatgggatc tctagagcgg gcccggtact aaccaaactg gatctctgct
     6181 gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt
     6241 ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc
     6301 cccttttctt ttaaaattgt ggatgaatac tgccatttgt ctgcagaatt ggcgcacgca
     6361 gtgccgatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt
     6421 ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg
     6481 gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac
     6541 ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa tttgttatgt
     6601 taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt
     6661 cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt
     6721 ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc
     6781 aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc
     6841 ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct
     6901 tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat
     6961 tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg
     7021 acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc
     7081 ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta
     7141 atggttcaat ttttactact ttatatttat ataattcact tctccaattg tccctcatat
     7201 ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc tgtgcggtgg tcttactttt
     7261 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
     7321 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
     7381 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
     7441 gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
     7501 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
     7561 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     7621 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     7681 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     7741 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     7801 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     7861 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     7921 gacccagtac aggcaaaacg cgctgcttat atagacctcc caccgtacac gcctaccgcc
     7981 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
     8041 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
     8101 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
     8161 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     8221 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     8281 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     8341 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     8401 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     8461 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
     8521 tcaacgcgta tatctggccc gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag
     8581 attcttcatg caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca
     8641 ctactcagcg acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc
     8701 agagcagatt gtactgagag tgcaccatag gggatcggga gatctcccga tccgtcgacg
     8761 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8821 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8881 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8941 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     9001 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     9061 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     9121 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     9181 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     9241 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     9301 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9361 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9421 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9481 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9541 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9601 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9661 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9721 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9781 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9841 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9901 gtagaaaaga tcaaaggatc ttc
//
