LOCUS       sequence-450088-        7444 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Transcription factor SNAI2 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7444)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 7444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-SNAI2-Brd 84
FEATURES             Location/Qualifiers
     source          1..7444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    136..257
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(173..192)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     227..246
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      297..432
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(452..471)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(453..471)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(481..498)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(481..497)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(490..512)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      639..1094
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(726..745)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     936..957
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1120..1224
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1225..1293,1294..2085)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1443..1462)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2256..2844
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2745..2764
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2998..3015
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3132..3153
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3168..3173,3174..3191,3192..3198)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3206..3222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3211..3233
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3230..3246
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3230..3246
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3265..3285
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3267..3285
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3313..3539
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3540..3720
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3767..3892
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4385..4618
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4803..4847
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5114..5231
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5265..5671
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    6754..7157
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             join(7275..7444,1..64)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
       61 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
      121 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
      181 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      241 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
      301 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
      361 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
      421 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
      481 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      541 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      601 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
      661 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      721 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      781 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
      841 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
      901 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
      961 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     1021 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     1081 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1141 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1201 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1261 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1321 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1381 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1441 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1501 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1561 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1621 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1681 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1741 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     1801 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     1861 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     1921 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     1981 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2041 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2101 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2161 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2221 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2281 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2341 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2401 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2461 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2521 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2581 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2641 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2701 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2761 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2821 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2881 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2941 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3001 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3061 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3121 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     3181 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     3241 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     3301 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     3361 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     3421 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     3481 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     3541 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3601 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3661 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3721 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     3781 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     3841 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     3901 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     3961 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     4021 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     4081 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     4141 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     4201 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     4261 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     4321 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     4381 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     4441 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     4501 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     4561 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     4621 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     4681 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     4741 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     4801 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     4861 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     4921 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     4981 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     5041 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     5101 ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     5161 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     5221 ttcaaaattt tatcgatcac gagactagcc tcgagctggc tgcagtaacg ccattttgca
     5281 aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg ggtacatgaa
     5341 aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc ccggcccggg
     5401 gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa cagatggtcc
     5461 ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc caggctcccc
     5521 caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg cttctcgctt
     5581 ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc tcactcggcg
     5641 cgccagtcct ccgacagact gagtcggccg gtcgaatcaa gcttatcgat accgtcgacg
     5701 gatccgctag cccgcggttc gaaatgccgc gctccttcct ggtcaagaag catttcaacg
     5761 cctccaaaaa gccaaactac agcgaactgg acacacatac agtgattatt tccccgtatc
     5821 tctatgagag ttactccatg cctgtcatac cacaaccaga gatcctcagc tcaggagcat
     5881 acagccccat cactgtgtgg actaccgctg ctccattcca cgcccagcta cccaatggcc
     5941 tctctcctct ttccggatac tcctcatctt tggggcgagt gagtccccct cctccatctg
     6001 acacctcctc caaggaccac agtggctcag aaagccccat tagtgatgaa gaggaaagac
     6061 tacagtccaa gctttcagac ccccatgcca ttgaagctga aaagtttcag tgcaatttat
     6121 gcaataagac ctattcaact ttttctgggc tggccaaaca taagcagctg cactgcgatg
     6181 cccagtctag aaaatctttc agctgtaaat actgtgacaa ggaatatgtg agcctgggcg
     6241 ccctgaagat gcatattcgg acccacacat taccttgtgt ttgcaagatc tgcggcaagg
     6301 cgttttccag accctggttg cttcaaggac acattagaac tcacacgggg gagaagcctt
     6361 tttcttgccc tcactgcaac agagcatttg cagacaggtc aaatctgagg gctcatctgc
     6421 agacccattc tgatgtaaag aaataccagt gcaaaaactg ctccaaaacc ttctccagaa
     6481 tgtctctcct gcacaaacat gaggaatctg gctgctgtgt agcacactga accggtcgga
     6541 tcccccgggc tgcaggaatt cgagcatctt accgccattt attcccatat ttgttctgtt
     6601 tttcttgatt tgggtataca tttaaatgtt aataaaacaa aatggtgggg caatcattta
     6661 catttttagg gatatgtaat tactagttca ggtgtattgc cacaagacaa acatgttaag
     6721 aaactttccc gttatttacg ctctgttcct gttaatcaac ctctggatta caaaatttgt
     6781 gaaagattga ctgatattct taactatgtt gctcctttta cgctgtgtgg atatgctgct
     6841 ttaatgcctc tgtatcatgc tattgcttcc cgtacggctt tcgttttctc ctccttgtat
     6901 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtccgtca acgtggcgtg
     6961 gtgtgctctg tgtttgctga cgcaaccccc actggctggg gcattgccac cacctgtcaa
     7021 ctcctttctg ggactttcgc tttccccctc ccgatcgcca cggcagaact catcgccgcc
     7081 tgccttgccc gctgctggac aggggctagg ttgctgggca ctgataattc cgtggtgttg
     7141 tcggggaagc tgacgtcctt tgcaattcga tatcaagctg taccttctag atgtacagaa
     7201 cttgtagcgc ttctgaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7261 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     7321 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7381 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7441 tctg
//
