LOCUS       sequence-450150-       11542 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Label telomere, no adhesion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Strom AR, Kim Y, Zhao H, Chang YC, Orlovsky ND, Kosmrlj A, Storm C, 
            Brangwynne CP
  TITLE     Condensate interfacial forces reposition DNA loci and probe 
            chromatin viscoelasticity.
  JOURNAL   Cell. 2024 Aug 14:S0092-8674(24)00828-6. doi: 
            10.1016/j.cell.2024.07.034.
  PUBMED    39168125
REFERENCE   2  (bases 1 to 11542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Aug 14:S0092-8674(24)00828-6. doi: 10.1016/j.cell.2024.07.034."
COMMENT     Sequence Label: miRFP670-TRF1
FEATURES             Location/Qualifiers
     source          1..11542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..1665)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1684..1731)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1779..2108)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1793..1928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2093..2113
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2122..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2232..2253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2444..2463
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2596..2820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2809..2826
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             2852..3032
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     3537..3553
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3538..3554
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3557..4145
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3663..3674
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4072..4092
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4208..5155)
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     promoter        complement(6546..7757)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(6690..6709)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    7822..7939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8237..8281)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8466..8699
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9192..9317
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9364..9544
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9562..9760)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9587..9607)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9762..10141
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     10313..10332
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10407..10511
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10512..10580,10581..11372)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10730..10749)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacgggcc ctgctagaga ttttccacac tgactaaaag
     2881 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     2941 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     3001 caggctcaga tctggtctaa ccagagagac ccagtacagt ccggatgcag ctctcgggcc
     3061 atgtgatgaa atgctaggcg gctgtcaaac ctccactcta atacttctct ctccgggtca
     3121 tccatcccat gcaggctcac agggtgtaac aagcgggtgt tctctccttc attggcttct
     3181 tctaccttct cttgctcaac tggtactagc ttgtagcacc atccaaaggt cagtggatat
     3241 ctgatccctg gccctggtgt gtagttctgc caatcaggga agtagccttg tgtgtggtag
     3301 atccacagat caaggatatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc
     3361 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt
     3421 acctgaggtg tgactggaaa acccacctcc tcctcctctt gtgcttctag ccaggcacaa
     3481 tcagcattgg tagctgctgt attgctactt gtgattgctc catgtttttc taggtctcga
     3541 ggtcgacggt atcgatgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga
     3601 aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct
     3661 ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca
     3721 caggcgagca gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg
     3781 cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg
     3841 gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc
     3901 caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg
     3961 ggccacaact cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa
     4021 gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta
     4081 aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta atccagaggt
     4141 tgattatcga taagcttgat atcgaattct agagtcgcgg ccgctttact tgtacgatcc
     4201 cggcgcgtta gctctcaagc gcggtgatcc gcgtcgcgat cctttcggcg agccgtttgc
     4261 agatcgcccg cagctcgaaa cggatgaagc gcggcagata atggtgacag acaaccaggc
     4321 cccacagctt gccgccgacc accagcgaca ccgccagggt ggcgcgcacg cccatgtcct
     4381 tcaggaactg cagatggcac ggcgacatcg agcgcaggaa gcagcccgac atgtcgagat
     4441 cgcgcccggt cagcggcgac agccgcggct ccagcggcac cggctgatag gtgacgtcga
     4501 ccagcacgcg gacgcgctgc cgcacgtaca gctgccgcgc catctgcggg acagtcgacg
     4561 acggatagcg gttgccgaaa taggattcga gcccaggcac atggcactcg gagaatacca
     4621 ggccgtggcc ttgctcatcg aaacgataca ccatcacccg gtcgtagccg gtgcactgct
     4681 gaaacagcag cacggtgtca tcgcacagcg cgcgcagtga acccgccgtg cggatccgct
     4741 ccagcgccgg cgccagcgtg cctgacagat cgatcgacgg gccggcacgt tcgagttcga
     4801 tgatcagccc gccttccgga ggccgatgca tcagaccgca gtactccgta gagggattgc
     4861 cgatccggca gcgcaccgcg accggcatgc cttcggcggt gggatcgaga tgcggcagga
     4921 tcttgatcaa cagatcgccg tcgatctcgg cgagcggaac gccgagtacg cttccgagat
     4981 tcagaaattc cgcggcgttg gcgctggcct ggatgacgcg atgatcatgt tcgctgacga
     5041 ccagaagcgc gccatgcggc tggatcgagc cggcgaggtg gatctcttca tgttcgcaat
     5101 tcgaatgaga ggcggtcccg aatgcggggc tgccagaggc atgacctgct accatactgc
     5161 cgctaccact accgctagag tcttcgctgt ctgaggaaat cagttttagt ttcttcatgg
     5221 tcctccatct gtcttttaac atgacacttg tccggttgtt gaatttataa tgcaacagta
     5281 ttttagacca gtttccctct ccatatttcc tcacgccaga tctcaaattc ttgtcttctt
     5341 cccaaagcca tgcctgtctt tttctagctc gatgtttttc aggagttacc ggctgactct
     5401 ttgaaacagg tattctgctt tcagtggctc ttctgctttc ttttttcttt tttgtacttt
     5461 gaggagttcc tactcttctt tctttcttat taaggtcttg ttgctgggtt ccatgttgca
     5521 acttagataa gaaaagattc ttgtgagacc tcaataagga tactgtaccc tctgaggatt
     5581 cagttaccgc agactgtttg tcactaacac tttttcttgt atccaaatta gcttcagttt
     5641 ccatttcaac atcattacca ctaggtttat cttgagaagt tattgttctt gtccttttgc
     5701 tttctactac ttttgccgct gccttcatta gaaaggttga tgatttttca cttagcacat
     5761 aattcacata actcttaatt ttctccatca tgtggttgta gctgaagtgt tgaaaaaagg
     5821 aatgaaatgt atctttctga gagattatca taagcaattt gcttttgaaa ggcatatgag
     5881 aatttggatc accaaatatt ctttcaaaga cttcttctgc ttctttaaag ttgccatttt
     5941 ccatacaaac agctatagcc tgaattttaa ttaaattctg tatttcttca tgaagtttgt
     6001 catgttcctt ttcaattgaa ccccaaatca tcagggctga ttccaagggt gtaattcgtt
     6061 catcattttc aaactgtgca tcaagggttt ttcctgctgc aattcttgtc aaaaactgac
     6121 atatgtatat cgttctcaac tggcaagctg ttagactgga tagtccatga ataatagcct
     6181 ctgcgctgtt gcgggtcctg cggaagtcct cggagcggcc gtcgcggaaa gctcggcaaa
     6241 gagagaggca gaggaaatcg agcatccagc cggcagccac ggcctcggcc tcggccacca
     6301 ggcccgcgtc ctcctcctcc tcctcctcct cctcgggggc ccccacctgc acctggcact
     6361 cgagcagttc ctggcattcg aactgctcct cgtcgtttct ctctgtttct gccatctgct
     6421 cctcagtagg gtcggcatcc ctaccatccg cacagccccg cgggctcggg gccgctgagg
     6481 aaacatcctc cgccatggtg ctagctaccg gtgatcctct agagtcgacc tgcagcccaa
     6541 gcttcgtcta acaaaaaagc caaaaacggc cagaatttag cggacaattt actagtctaa
     6601 cactgaaaat tacatattga cccaaatgat tacatttcaa aaggtgccta aaaaacttca
     6661 caaaacacac tcgccaaccc cgagcgcata gttcaaaacc ggagcttcag ctacttaaga
     6721 agataggtac ataaaaccga ccaaagaaac tgacgcctca cttatccctc ccctcaccag
     6781 aggtccggcg cctgtcgatt caggagagcc taccctaggc ccgaaccctg cgtcctgcga
     6841 cggagaaaag cctaccgcac acctaccggc aggtggcccc accctgcatt ataagccaac
     6901 agaacgggtg acgtcacgac acgacgaggg cgcgcgctcc caaaggtacg ggtgcactgc
     6961 ccaacggcac cgccataact gccgcccccg caacagacga caaaccgagt tctccagtca
     7021 gtgacaaact tcacgtcagg gtccccagat ggtgccccag cccatctcac ccgaataaga
     7081 gctttcccgc attagcgaag gcctcaagac cttgggttct tgccgcccac catgcccccc
     7141 accttgtttc aacgacctca cagcccgcct cacaagcgtc ttccattcaa gactcgggaa
     7201 cagccgccat tttgctgcgc tccccccaac ccccagttca gggcaacctt gctcgcggac
     7261 ccagactaca gcccttggcg gtctctccac acgcttccgt cccaccgagc ggcccggcgg
     7321 ccacgaaagc cccggccagc ccagcagccc gctactcacc aagtgacgat cacagcgatc
     7381 cacaaacaag aaccgcgacc caaatcccgg ctgcgacgga actagctgtg ccacacccgg
     7441 cgcgtcctta tataatcatc ggcgttcacc gccccacgga gatccctccg cagaatcgcc
     7501 gagaagggac tacttttcct cgcctgttcc gctctctgga aagaaaacca gtgccctaga
     7561 gtcacccaag tcccgtccta aaatgtcctt ctgctgatac tggggttcta aggccgagtc
     7621 ttatgagcag cgggccgctg tcctgagcgt ccgggcggaa ggatcaggac gctcgctgcg
     7681 cccttcgtct gacgtggcag cgctcgccgt gaggaggggg gcgcccgcgg gaggcgccaa
     7741 aacccggcgc ggaggccaga tctggagccg acacgggtta attaaccaaa ctggatctct
     7801 gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt
     7861 agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat
     7921 cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtctgcaga attggcgcac
     7981 gcagtgccga tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt
     8041 cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata
     8101 atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa actattctta
     8161 aacctaccaa gcctcctact atcattatga ataattttat ataccacagc caatttgtta
     8221 tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct tgttcattct
     8281 tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa
     8341 tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac
     8401 tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa
     8461 tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct
     8521 gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca
     8581 aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca
     8641 ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta
     8701 ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc
     8761 ctaatggttc aatttttact actttatatt tatataattc acttctccaa ttgtccctca
     8821 tatctcctcc tccaggtctg aagatcagcg gccgcttgct gtgcggtggt cttacttttg
     8881 ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga
     8941 tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc
     9001 tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg
     9061 ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta
     9121 tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc
     9181 cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt
     9241 tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga
     9301 gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc
     9361 cactgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     9421 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     9481 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     9541 acccagtaca ggcaaaacgc gctgcttata tagacctccc accgtacacg cctaccgccc
     9601 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
     9661 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
     9721 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
     9781 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     9841 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     9901 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
     9961 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
    10021 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
    10081 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt
    10141 caacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga
    10201 ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac
    10261 tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcatca
    10321 gagcagattg tactgagagt gcaccatagg ggatcgggag atctcccgat ccgtcgacgt
    10381 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    10441 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    10501 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    10561 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    10621 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    10681 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    10741 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    10801 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10861 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10921 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10981 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    11041 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    11101 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    11161 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    11221 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    11281 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    11341 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    11401 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    11461 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    11521 tagaaaagat caaaggatct tc
//
