LOCUS       sequence-450282-       14406 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expressing Trachelospermum jasminoides FLS2 protein in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14406)
  TITLE     Coaker lab FLS2 plasmids
REFERENCE   2  (bases 1 to 14406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB514-35S:TjFLS2-HA
FEATURES             Location/Qualifiers
     source          1..14406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     489..508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     742..759
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    774..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1258..1452
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1518..2591)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3020..3649)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4950..4974
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5162..5184
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5176..5193
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5177..5193
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5708..6053
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5996..6016
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    6075..6099
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10049..10073)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10102..10191
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      10225..10477
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(10510..10526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10510..10526)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10523..10545)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10534..10550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10558..10564,10565..10582,10583..10588))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10603..10624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(10660..10912)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11063..12088)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(12140..12319)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    12825..12849
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             13370..14161
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaaagcta ccaactcttt ttccgaaggt aactggcttc
      121 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
      181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
      781 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
     1081 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
     1141 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
     1201 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
     1261 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
     1321 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
     1381 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
     1441 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
     1501 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
     1561 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
     1621 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
     1681 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
     1741 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
     1801 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
     1861 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
     1921 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
     1981 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
     2041 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
     2101 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
     2161 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
     2221 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
     2281 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
     2341 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
     2401 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
     2461 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
     2521 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
     2581 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
     2641 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
     2701 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
     2761 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
     2821 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
     2881 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
     2941 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
     3001 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
     3061 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
     3121 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
     3181 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
     3241 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
     3301 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
     3361 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
     3421 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
     3481 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
     3541 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
     3601 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
     3661 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
     3721 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
     3781 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
     3841 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
     3901 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
     3961 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
     4021 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
     4081 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
     4141 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
     4201 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
     4261 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
     4321 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
     4381 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
     4441 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
     4501 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
     4561 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
     4621 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
     4681 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
     4741 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
     4801 gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt
     4861 tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga
     4921 ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact
     4981 gatagtttaa actgaaggcg ggaaacgaca atctgatcca agctcaagct gctctagcat
     5041 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     5101 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     5161 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct gcaggtcccc
     5221 agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga gaggcttacg
     5281 cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc aaataccttc
     5341 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga
     5401 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
     5461 acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc actgaatcaa
     5521 aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta aagactggcg
     5581 aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc gtcaacatgg
     5641 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
     5701 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
     5761 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
     5821 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
     5881 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     5941 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
     6001 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga acacggggga
     6061 ctctagagtt atcaacaagt ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca
     6121 tgactttaca tagagttttt cttcattcag tcatattatt ttgtgttttc tttgttgggg
     6181 ttctgccatt ggatctaagc tgggaatctg aagttgaggc tctgaaagca ttcaagagtt
     6241 caatcacaga tgatccattg agtgcattgg gagattggag taatgccaaa catcattgta
     6301 actggactgg aatcgcatgc catccttcaa cagatcatgt catttccatt tctctggttg
     6361 acaagcagct tcagggtgaa atctccccat ttatgggaaa tctttctcat ctccaggttc
     6421 ttgatctaac tttaaactca tttactggaa atattcctcg tgaactcggg ctctgctcgc
     6481 agctcaagga actgattctc tattcaaatt ctctttctgg agaaatcccg gaagagcttg
     6541 gaaatcttga aactttagag tcagtagatt ttggaaacaa tttattgaat ggtagcatac
     6601 cagatagcat atgcaattgt agatccctct tacaactagg cctcatctac aacaatctca
     6661 ctggggtagt accatctaac attggtaact tgaccaatct tcaactgttt gtagcttatg
     6721 ctaacaattt ggccggttct atacctgctt ccattggaaa tttgcaagca ttgcaagctc
     6781 ttgacctgag tgtaaaccaa ttatctggtc caataccacc agaaattgga aatttgacaa
     6841 acttagaggt ccttcaactg ttttctaatt ctctcaaagg gaaaatcccc tctgaacttg
     6901 gtcgttgtca aaagctagtg ttgcttaacg tatataccaa tcagttcact ggtggcattc
     6961 ctcctgaact tggaaacctg actaacttag aggcactgcg tctgtaccaa aataacttga
     7021 actccacaat cccacacacc ctgttccagc taaaatcatt aacccatctt ggactttcag
     7081 aaaataattt aatcggtagc atcccttctg agatcggaac tttgagatca ttacaagtac
     7141 tgaccctcca ttcaaacagg ttcacatgga atatcccatt atctgtaaca aacttgacaa
     7201 acctaacata cttgtctatg agcttcaatt cattgacagg gaagcttcta tcagatatag
     7261 ggtcactaca caacttaaag aacctaagcc tgaacaataa tcttctcgaa ggatctatac
     7321 catctagcat tagtaactgc acacatcttc ttgtcatatc actgacttac aacagactca
     7381 ccggaaaact acctgagggg ctaggacaaa tatctaacct gacatacttc tctgttggtg
     7441 gaaataaaat atcaggttac atacctgatg acctcttcaa ctgttcaatg ctccaagtcg
     7501 tagatttaag cctaaacaat tttagtggaa gaattaatcc gaatattgga aggctatcta
     7561 atctgcgcct tttccgagct catgacaatt cactgtctgg gtatatccca agtgagattg
     7621 gcaatctaag ccaattaatg gacttagttc ttcagaagaa caatttttca ggtccaattc
     7681 ctcctgaggt ttcaaaactt acagccctgc aaggccttgc actgaatcac aatttgctgg
     7741 aaggtgatat tccagaacaa ctttctgagc tcaaacaact cactgaactt cgtttgcaac
     7801 acaataagct cgttggtcca atcccagaag ctatctccaa gcttgagtta ctttcatact
     7861 tggatcttag cgaaaacatg ctcaatggaa caatcccaaa aggtatggaa caccttaggc
     7921 gattaatggc cttagatctg tcaagaaacc atctcactgg tactgttcct gctgcactca
     7981 tgggaaacct taaaagtctg cagatatatc tgaatctttc acacaattcc ttggagggaa
     8041 ctattccaga tgaatttggc aggttggaaa tggtgcaggt gatcgacatg tcaaacaata
     8101 atttgtcagg aagcattcct caaacccttg aacactgcag gaatctattc tctctagatc
     8161 tttctggaaa caaactctct ggtgaacttc cagaacaaac ttttcttcaa ctcagagagc
     8221 ttgtaacctt gaacctctca agaaatcaat tagaaggtgc acttcctgca gatctagcaa
     8281 gtctgagata tctcgagtcg cttgatcttt ctcaaaataa gttcaatggc cccattcctg
     8341 agagctttgc taatctctca aatttgagat atctcaacct ttctttcaat aaacttcaag
     8401 gtcgtgttcc agagaatggt gtattcagaa actcaagtgc aactactttg gttgggaacc
     8461 ctgcactatg tggaacaaga cttgtcattc cttgtgatga tggaaagcat ctaaaaaatt
     8521 tccatcatag aagaaagaaa gttttgatca ttctggtgac acttggatcc ttttgtttac
     8581 tcttcctctt ggtgtgggca atctctgtca ttcgttataa aaccagagca caaacagtaa
     8641 aagagcatgc gaacttggaa cgtaactata cttcagcttt gactctcaag agatttgatc
     8701 agaagaattt agaaattatg acaaatttct tcagtcagga aaacatcatt ggttccacca
     8761 gtttaagcac agtgtacaag ggtagtctgg aagatgggca gcttgtagca gtaaagaaat
     8821 ttaatctgca ccagttttca gtagaatcag acaagtgctt tgatagagaa gtcaaaactc
     8881 tctgccaatt aagacatagg aatcttgtta agatacttgg ctatgcttgg gagagtggaa
     8941 agctgaaagc tttagttcta gagtacatgg aaaatgggaa cttggataga atcatacatg
     9001 gccaaaacat tgagggttcc atatggacaa tatcagaaag gattgatact ttagtttcta
     9061 ttgcaagtgg tctggtatac ctgcattctg gctatgattt ccccatagtg cactgtgact
     9121 tgaaaccttc caatattctg ttggatggaa acttagttgc ccatgtcagt gattttggga
     9181 cagctaggat tcttggtatt catgtagaag atggaagtaa cctctcttca tcatcagcat
     9241 ttgaaggaac tattggctac ttggcaccag gtaactctta actactctta tttacttttc
     9301 acttttggct ctgtttctta tctataatta ctcactttta tgaaatcaat gagttattta
     9361 ctaaattttt tttttacaat tttaccctta tttcttaatc acctcacaag accatcacat
     9421 taattagttt tcattttcaa tcagggttag ttcagtcttt ttcttttatg agccatgata
     9481 ttatgacatt gttacaagtg tgatctagaa agtgaatgat cttatgcttg tttcttccta
     9541 ttcttagtga agtacacata aacaaagaga aaatatttac ttaaacaaat ctatttcaat
     9601 accagataat ctatgtaacc attctagttt caagtgaaac aacatgttat atctacctta
     9661 tgagaacaag tttatctctg tctctcagaa tttgcataca tgcgagcagt tacaacaaaa
     9721 gtagatgtat tcagctttgg tgtaataatg atggaattca ttacaaaaag aaggccaaca
     9781 ggacttacag aagaaggatc accaatcacc ttgcgccaac ttgtgcagaa aacctttgca
     9841 agtgggataa acagcttaat acaggccgtg gatcctcatt tagctccata tgtctccaag
     9901 aatcaggaag taatagaaag ccttcttagt ttagccttat cctgcaccaa cacagaacct
     9961 gaggaccggc ctaacatgga agaagtattg tcttgccttt caaaggtaag taagctttcg
    10021 caaggaggtt ctaagggtgg gcgcgccgac ccagctttct tgtacaaagt ggttgataac
    10081 agcgggttaa ttaacatctt ttacccatac gatgttcctg actatgcggg ctatccctat
    10141 gacgtcccgg actatgcagg atcctatcca tatgacgttc cagattacgc tgctcagtgc
    10201 agcgcttaga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga
    10261 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
    10321 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
    10381 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
    10441 aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattggttcc ggaaccaatt
    10501 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    10561 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    10621 attaggctga attaggcgcg cctatttcta ttaattcccg atctagtaac atagatgaca
    10681 ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa
    10741 tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca
    10801 tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac
    10861 cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga tcggggaaat
    10921 tcgggggatc gatcccgcga actgtggacg agaactgtgc caccaagcgt aaggccgttc
    10981 tctcgcattt gccttgctag gctcgcgcga gttgctggct gaggcgttct cgaaatcagc
    11041 tcttgttcgg tcggcatcta ctctattcct ttgccctcgg acgagtgctg gggcgtcggt
    11101 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg
    11161 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct
    11221 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac
    11281 caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct
    11341 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
    11401 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc
    11461 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt
    11521 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga
    11581 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga
    11641 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
    11701 tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag
    11761 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc
    11821 aataggtcag gctctcgctg aatgccccaa tgtcaagcac ttccggaatc gggagcgcgg
    11881 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta
    11941 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
    12001 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag
    12061 acgtcgcggt gagttcaggc tttttcatat cttattgccc cccgggatca gatctggatt
    12121 gagagtgaat atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc
    12181 atttttgaca agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa
    12241 taatggtttc tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc
    12301 tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg
    12361 cgggggtcat aacgtgactc ccttaattct ccgctcatga tcttgatccc ctgcgccatc
    12421 agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag
    12481 agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct
    12541 atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg
    12601 tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt
    12661 tctacgtgtt ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag
    12721 ctgatccgtc gagattaata gaaataaatt cagcctaatt cggcgttaat tcagtacatt
    12781 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
    12841 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
    12901 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
    12961 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
    13021 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
    13081 gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
    13141 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
    13201 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
    13261 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
    13321 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
    13381 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
    13441 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
    13501 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
    13561 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
    13621 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
    13681 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
    13741 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
    13801 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
    13861 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
    13921 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
    13981 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
    14041 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
    14101 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
    14161 atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg
    14221 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat
    14281 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa
    14341 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    14401 atcttc
//
