LOCUS       sequence-450286-       14284 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expressing SynFcFLS2_13 protein in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14284)
  TITLE     Coaker lab FLS2 plasmids
REFERENCE   2  (bases 1 to 14284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB514-35S:SynFcFLS2_13-HA
FEATURES             Location/Qualifiers
     source          1..14284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             127..918
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1164..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1937..2077
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2003..2022)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2163..2185
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2421..2615
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2681..3754)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4183..4812)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6113..6137
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6325..6347
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6339..6356
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6340..6356
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6871..7216
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7159..7179
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    7238..7262
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11090..11114)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11143..11232
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      11266..11518
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(11551..11567)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11551..11567)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11564..11586)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11575..11591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11599..11605,11606..11623,11624..11629))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11644..11665
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(11701..11953)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12104..13129)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(13181..13360)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    13866..13890
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
       61 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      121 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
      181 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
      241 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
      301 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
      361 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
      421 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
      481 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
      541 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
      601 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
      661 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
      721 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
      781 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
      841 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
      901 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc
      961 gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     1021 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     1081 atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1141 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1201 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     1981 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     2041 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     2101 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     2161 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     2221 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     2281 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     2341 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     2401 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     2461 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     2521 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     2581 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     2641 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     2701 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     2761 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     2821 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     2881 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     2941 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     3001 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
     3061 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
     3121 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
     3181 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
     3241 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
     3301 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
     3361 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
     3421 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
     3481 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
     3541 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
     3601 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
     3661 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
     3721 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
     3781 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
     3841 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
     3901 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
     3961 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
     4021 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
     4081 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
     4141 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
     4201 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
     4261 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
     4321 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
     4381 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
     4441 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
     4501 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
     4561 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
     4621 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
     4681 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
     4741 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
     4801 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
     4861 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
     4921 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
     4981 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
     5041 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
     5101 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
     5161 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
     5221 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
     5281 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
     5341 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
     5401 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
     5461 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
     5521 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
     5581 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
     5641 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
     5701 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
     5761 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
     5821 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
     5881 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
     5941 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
     6001 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt
     6061 ttcacgccct tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc
     6121 gccaatatat cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat
     6181 ccaagctcaa gctgctctag cattcgccat tcaggctgcg caactgttgg gaagggcgat
     6241 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     6301 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
     6361 agcttgcatg cctgcaggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc
     6421 acagatggtt agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa
     6481 tctccaggaa atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac
     6541 taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt
     6601 atggacgatt caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa
     6661 aaaggtagtt cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac
     6721 agaactcgcc gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa
     6781 gaagaaaatc ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa
     6841 agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg
     6901 aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa
     6961 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc
     7021 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     7081 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     7141 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     7201 tcatttggag agaacacggg ggactctaga gttatcaaca agtttgtaca aaaaagcagg
     7261 ctccgcggcc gcccccttca ccatggtatc tcaaagagtc agtttgattg tactcatagc
     7321 ttgttccctt ctagttactg ttctatcaga agaatcaagc ttagaagttc aacttgaggc
     7381 cttgaaggct ttcaagaatt ccatcaccaa tgacccaagt ggagcacttg tggattggac
     7441 tgacaggaat caccactgca actggtctgg tattgcttgt gacccttctt caaattatgt
     7501 cgtttcgatt tctctcattg ataagcagct caaaggtgaa attacaccat tccttggaaa
     7561 cctttcaagc ctccaggttc ttgatttaac ttcaaataca ttcacaggcc acattccagc
     7621 tcaactggga ctttgctccc agctctctgt gctaactctt tatgaaaatt ctctctcagg
     7681 tccaattcca ccagaactag gaaacctcaa aaatctgcag tcagtagatt taggtagtaa
     7741 ctttttgaat ggaagcatcc ctgaaagcat ttgcaactgc acatcattgt tagcatgttc
     7801 tgtaagtttt aacaatctag ctggcattat cccttcaaac ataggtaatt tagctaacct
     7861 tcaaatcttt ttagtatata agaacaattt ggtaggttct atacctgtgt ccattggtag
     7921 gctggaatct ttgcaagctt tagacctgag tcaaaaccaa ttctctggag ttatacctcc
     7981 acagatagga aacttttcga atttagagta tcttctcttg tttgaaaatt cctttgttgg
     8041 gaaaataccg tctgaactag gtcgctgtaa aaagcttgtt gctcttgaac tatacaccaa
     8101 tcagttcact ggaggcattc catttgagat tggaaactta gttcacctag aaacattgag
     8161 gttgtacaat aataagttaa attctaccat tcctgcctcc ttattgcaat tgaaatcatt
     8221 aacccattta ggactttcag aaaacgagtt aacgggaatt ataccatcag aacttggctc
     8281 tttgcgaagt ctcgtcgttt taaccttgca ttcaaataaa ttttctgggg aaattccatc
     8341 tagtcttacg aacttgacga atttaacgca gcttagcatg tcctttaact ttttaaccgg
     8401 aaagttgcca tctgatatcg ggttgctgta taacctgaaa aacctcacta tgaacaataa
     8461 cctccttgag ggaacaatcc ctagcagcat caagaactgt acgcaccttc tcgtcgtggg
     8521 gctttctcgt aacaaaatta ctggaaaaat accctggggt tttcagcaac tcctgaactt
     8581 aacattctta tcactgagct acaacaaaat gagtgatgaa atcccagacg atcttttcaa
     8641 ttgtagcaat ctgcgtattc tggatttgac acaaaacttc ttcagtggac ccattaaaag
     8701 cggcatcgga aagttatcaa acttacagat tctgcgtgtg catacaaatt ccttcatcgg
     8761 gcctattcca ccagatatgg gaaacctttc taaattgttt agtctcagct tagggcaaaa
     8821 ttctttgagt ggtctcgtac caagtgagct gagtaagtta agcctgttgc aggggctgta
     8881 ccttaatgat aacgctttag aaggggcaat ccctgagaag attttcgagg taaagcagtt
     8941 aaatgaggtt ggtttgcaga ataataggtt caagggtccg attccagatg cagtttctaa
     9001 gcttgagcag ctttcatact tagacctcca tggtaacatg ctaaacgggt ccattctgag
     9061 aagcatggag catcttaata aacttatgac cttggatctc tcccacaacc atcttacagg
     9121 atcaatacct tcattggtga ttgcaagtat gaaaaccatg cagatatatt tgaacttgtc
     9181 atacaatttc ttggttggaa ctattccaga tgagcttggg acgttggaaa cagtacaggc
     9241 cattgacatc tcaaacaata atctttcggg gatcattcct aaaacactcc gtggctgcag
     9301 aaatttgttc tctcttgatc tgtcagggaa caaactttct ggtccaattc cagctgaagc
     9361 tttaactcaa atggatctgc ttactagctt gaacctttca aggaacaagt tagatagtgg
     9421 actccctgaa gaccttgcaa gtctaaagca ccttatctcc cttgaccttt ctcagaacca
     9481 gttgaagggc attatcccgg agagctttgc caatctctcc accttgaaac atctcaacct
     9541 ttctttcaat caacttgaag gtcatgttcc agagattgga atatttagac acattaactc
     9601 atctagtttg atgggaaacc cggatctgtg tggaactgaa ttcctcagat cctgcagcaa
     9661 cttgagttta cataagctat ccaagaagac catggtgatt cttggggtac ttggagcaat
     9721 ttttgtactt ctagttctag tgttcgtaat tatgatcctc aaacgacgta ccaagttgag
     9781 taaatcagaa gaggttgaga atcctgaatc agaatatact ccagcattga ccctgaagag
     9841 gtttgtacca aacgaattag aacatgctac tagttttttt agcaaagata acattattgg
     9901 tgccagcaat ttgagcactg tgtacaaggg tagactggaa gatgggcaga tcatagctgt
     9961 taaaaagttg aatttgcatc agttctctga agagtctgat aggtgctttt atagagagat
    10021 caacaccctg aaacaactga ggcataggaa tttggtgaag gttctaggct atgcttggga
    10081 gagtggaaaa ctaaaggctt tagttttaga atacatggag aatgggaact tggatagcat
    10141 tatacatgac cctgcggtgg atcagtcaag gtggacattg tctgagagaa tcaaagtttt
    10201 catttccatt gcaactgggt tggattacct gcattcaggt tatgatttcc ctatcgttca
    10261 ttgtgacatg aagccttcta acattcttct ggatgaagat tgggaagccc atgtgagtga
    10321 ctttggaaca gctcgaatgc taggagttca tcttcacgac ggaagcagtc tttctgcaac
    10381 agcagcattt gaaggcacta ttggctactt ggcaccaggt aacttatcac cactccttct
    10441 cagttatgaa gttaatcccc attcttattg ctgtcaaatt gcaaatcatc ttgttattgt
    10501 aaattataag cagaaaaaga gtgaaacttt caacaatcac aatcagtttg gggcaacaat
    10561 tcagtcttgc acatataaat accgggttca catattaaac tgaaatccta agcattgcat
    10621 atatgtctat agtgcacata taaataccag aggtacatat gaagttactg aattcaaata
    10681 ccatatgtct gtcttggccc tcaatatatt aaaaacatgt ttgttttctt accagagttt
    10741 gcgtacatgc cgaaagtcac aactaaagtt gacatattca gctttggtat aatagtcatg
    10801 gagtttctca cgaaacgaag gccaacagca ctaatggaag aggatggttt gccaatcagt
    10861 ttgcgtcaac tcgtggagaa agctcttgta aatggagtga acagtcttcc tcgaattctg
    10921 gactctatgc tggtgttgaa tatctccaag gagcatgagg aagtattgaa agagttactt
    10981 aaaatagcat tgctttgcac agaaccaaat cctgaggatc gacccgacat aaatgaggtg
    11041 ttgtctatcc ttttgaagct aagcaaacgc ataaagggtg ggcgcgccga cccagctttc
    11101 ttgtacaaag tggttgataa cagcgggtta attaacatct tttacccata cgatgttcct
    11161 gactatgcgg gctatcccta tgacgtcccg gactatgcag gatcctatcc atatgacgtt
    11221 ccagattacg ctgctcagtg cagcgcttag agctcgaatt tccccgatcg ttcaaacatt
    11281 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
    11341 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
    11401 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
    11461 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
    11521 gaattggttc cggaaccaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
    11581 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    11641 ctaatgagtg agctaactca cattaggctg aattaggcgc gcctatttct attaattccc
    11701 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
    11761 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
    11821 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
    11881 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
    11941 tgtttgaacg atcggggaaa ttcgggggat cgatcccgcg aactgtggac gagaactgtg
    12001 ccaccaagcg taaggccgtt ctctcgcatt tgccttgcta ggctcgcgcg agttgctggc
    12061 tgaggcgttc tcgaaatcag ctcttgttcg gtcggcatct actctattcc tttgccctcg
    12121 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
    12181 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
    12241 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
    12301 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
    12361 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
    12421 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
    12481 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
    12541 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
    12601 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
    12661 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
    12721 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
    12781 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
    12841 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaatgcccca atgtcaagca
    12901 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
    12961 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
    13021 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
    13081 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tcttattgcc
    13141 ccccgggatc agatctggat tgagagtgaa tatgagactc taattggata ccgaggggaa
    13201 tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt
    13261 aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc
    13321 agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa
    13381 cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg
    13441 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
    13501 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
    13561 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
    13621 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
    13681 accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
    13741 agtgcttgcg gcagcgtgaa gctgatccgt cgagattaat agaaataaat tcagcctaat
    13801 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
    13861 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
    13921 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
    13981 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
    14041 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
    14101 gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg
    14161 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
    14221 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
    14281 tttt
//
