LOCUS       sequence-450296-       14284 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expressing Fagus crenata FLS2 protein in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14284)
  TITLE     Coaker lab FLS2 plasmids
REFERENCE   2  (bases 1 to 14284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB514-35S:FcFLS2-HA
FEATURES             Location/Qualifiers
     source          1..14284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     489..508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     742..759
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    774..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1258..1452
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1518..2591)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3020..3649)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4950..4974
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5162..5184
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5176..5193
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5177..5193
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5708..6053
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5996..6016
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    6075..6099
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9927..9951)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9980..10069
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      10103..10355
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(10388..10404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10388..10404)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10401..10423)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10412..10428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10436..10442,10443..10460,10461..10466))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10481..10502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(10538..10790)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(10941..11966)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(12018..12197)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    12703..12727
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             13248..14039
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaaagcta ccaactcttt ttccgaaggt aactggcttc
      121 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
      181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
      781 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
     1081 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
     1141 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
     1201 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
     1261 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
     1321 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
     1381 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
     1441 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
     1501 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
     1561 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
     1621 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
     1681 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
     1741 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
     1801 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
     1861 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
     1921 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
     1981 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
     2041 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
     2101 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
     2161 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
     2221 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
     2281 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
     2341 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
     2401 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
     2461 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
     2521 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
     2581 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
     2641 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
     2701 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
     2761 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
     2821 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
     2881 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
     2941 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
     3001 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
     3061 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
     3121 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
     3181 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
     3241 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
     3301 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
     3361 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
     3421 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
     3481 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
     3541 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
     3601 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
     3661 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
     3721 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
     3781 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
     3841 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
     3901 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
     3961 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
     4021 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
     4081 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
     4141 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
     4201 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
     4261 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
     4321 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
     4381 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
     4441 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
     4501 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
     4561 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
     4621 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
     4681 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
     4741 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
     4801 gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt
     4861 tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga
     4921 ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact
     4981 gatagtttaa actgaaggcg ggaaacgaca atctgatcca agctcaagct gctctagcat
     5041 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     5101 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     5161 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct gcaggtcccc
     5221 agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga gaggcttacg
     5281 cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc aaataccttc
     5341 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga
     5401 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
     5461 acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc actgaatcaa
     5521 aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta aagactggcg
     5581 aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc gtcaacatgg
     5641 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
     5701 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
     5761 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
     5821 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
     5881 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     5941 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
     6001 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga acacggggga
     6061 ctctagagtt atcaacaagt ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca
     6121 tggtatctca aagagtcagt ttgattgtac tcatagcttg ttcccttcta gttactgttc
     6181 tatcagaaga atcaagctta gaagttcaac ttgaggcctt gaaggctttc aagaattcca
     6241 tcaccaatga cccaagtgga gcacttgtgg attggactga caggaatcac cactgcaact
     6301 ggtctggtat tgcttgtgac ccttcttcaa attatgtcgt ttcgatttct ctcattgata
     6361 agcagctcaa aggtgaaatt acaccattcc ttggaaacct ttcaagcctc caggttcttg
     6421 atttaacttc aaatacattc acaggccaca ttccagctca actgggactt tgctcccagc
     6481 tctctgtgct aactctttat gaaaattctc tctcaggtcc aattccacca gaactaggaa
     6541 acctcaaaaa tctgcagtca gtagatttag gtagtaactt tttgaatgga agcatccctg
     6601 aaagcatttg caactgcaca tcattgttag catgttctgt aagttttaac aatctagctg
     6661 gcattatccc ttcaaacata ggtaatttag ctaaccttca aatcttttta gtatataaga
     6721 acaatttggt aggttctata cctgtgtcca ttggtaggct ggaatctttg caagctttag
     6781 acctgagtca aaaccaattc tctggagtta tacctccaca gataggaaac ttttcgaatt
     6841 tagagtatct tctcttgttt gaaaattcct ttgttgggaa aataccgtct gaactaggtc
     6901 gctgtaaaaa gcttgttgct cttgaactat acaccaatca gttcactgga ggcattccat
     6961 ttgagattgg aaacttagtt cacctagaaa cattgaggtt gtacaataat aagttaaatt
     7021 ctaccattcc tgcctcctta ttgcaattga aatcattaac ccatttagga ctttcacaga
     7081 atgagttaac tggaataata ccctctgagc taggatcatt gagatcattg gtagtgttga
     7141 ccctgcattc aaataagttc tctggggaga ttccttcgtc attgacgaac ttgacaaatt
     7201 taactcagtt gtctatgagc ttcaattttc tcacagggaa gcttccatca gatattggat
     7261 tgctttacaa tttgaagaac ctaaccatga acgacaacct ccttgaggga accattccat
     7321 ctagtatcaa gaactgcacc catcttttag tggttggttt agctaataac aaaatcacag
     7381 ggaaaattcc ttggggtttt cagcagttgc taaatcttac ttccctgtct ctttcatcca
     7441 ataaaatgtc agatgaaatc cctgatgacc tcttcaattg ctccaatctt cgcattttaa
     7501 atttggctct gaactttttt agtggaccaa taaagtctgg cattggcaaa ctttctaatc
     7561 tcgagatttt gcgagtccat acgaattcat tcatagggcc aatcccacct gacatgggca
     7621 atttaagtaa acttatttct ttatcacttg gtcaaaatag tttgtcaggt ctagttccat
     7681 cagaattatc taagctttcc cttctccaag ggctctacct gaatgacaat gctctagagg
     7741 gtgcaatacc tgagaaaatt tttgaagtga aacagctaag cgaagttgca ttgcagaata
     7801 ataggttcaa gggtccgatt ccagatgcag tttctaagct tgagcagctt tcatacttag
     7861 acctccatgg taacatgcta aacgggtcca ttctgagaag catggagcat cttaataaac
     7921 ttatgacctt ggatctctcc cacaaccatc ttacaggatc aataccttca ttggtgattg
     7981 caagtatgaa aaccatgcag atatatttga acttgtcata caatttcttg gttggaacta
     8041 ttccagatga gcttgggacg ttggaaacag tacaggccat tgacatctca aacaataatc
     8101 tttcggggat cattcctaaa acactccgtg gctgcagaaa tttgttctct cttgatctgt
     8161 cagggaacaa actttctggt ccaattccag ctgaagcttt aactcaaatg gatctgctta
     8221 ctagcttgaa cctttcaagg aacaagttag atagtggact ccctgaagac cttgcaagtc
     8281 taaagcacct tatctccctt gacctttctc agaaccagtt gaagggcatt atcccggaga
     8341 gctttgccaa tctctccacc ttgaaacatc tcaacctttc tttcaatcaa cttgaaggtc
     8401 atgttccaga gattggaata tttagacaca ttaactcatc tagtttgatg ggaaacccgg
     8461 atctgtgtgg aactgaattc ctcagatcct gcagcaactt gagtttacat aagctatcca
     8521 agaagaccat ggtgattctt ggggtacttg gagcaatttt tgtacttcta gttctagtgt
     8581 tcgtaattat gatcctcaaa cgacgtacca agttgagtaa atcagaagag gttgagaatc
     8641 ctgaatcaga atatactcca gcattgaccc tgaagaggtt tgtaccaaac gaattagaac
     8701 atgctactag tttttttagc aaagataaca ttattggtgc cagcaatttg agcactgtgt
     8761 acaagggtag actggaagat gggcagatca tagctgttaa aaagttgaat ttgcatcagt
     8821 tctctgaaga gtctgatagg tgcttttata gagagatcaa caccctgaaa caactgaggc
     8881 ataggaattt ggtgaaggtt ctaggctatg cttgggagag tggaaaacta aaggctttag
     8941 ttttagaata catggagaat gggaacttgg atagcattat acatgaccct gcggtggatc
     9001 agtcaaggtg gacattgtct gagagaatca aagttttcat ttccattgca actgggttgg
     9061 attacctgca ttcaggttat gatttcccta tcgttcattg tgacatgaag ccttctaaca
     9121 ttcttctgga tgaagattgg gaagcccatg tgagtgactt tggaacagct cgaatgctag
     9181 gagttcatct tcacgacgga agcagtcttt ctgcaacagc agcatttgaa ggcactattg
     9241 gctacttggc accaggtaac ttatcaccac tccttctcag ttatgaagtt aatccccatt
     9301 cttattgctg tcaaattgca aatcatcttg ttattgtaaa ttataagcag aaaaagagtg
     9361 aaactttcaa caatcacaat cagtttgggg caacaattca gtcttgcaca tataaatacc
     9421 gggttcacat attaaactga aatcctaagc attgcatata tgtctatagt gcacatataa
     9481 ataccagagg tacatatgaa gttactgaat tcaaatacca tatgtctgtc ttggccctca
     9541 atatattaaa aacatgtttg ttttcttacc agagtttgcg tacatgccga aagtcacaac
     9601 taaagttgac atattcagct ttggtataat agtcatggag tttctcacga aacgaaggcc
     9661 aacagcacta atggaagagg atggtttgcc aatcagtttg cgtcaactcg tggagaaagc
     9721 tcttgtaaat ggagtgaaca gtcttcctcg aattctggac tctatgctgg tgttgaatat
     9781 ctccaaggag catgaggaag tattgaaaga gttacttaaa atagcattgc tttgcacaga
     9841 accaaatcct gaggatcgac ccgacataaa tgaggtgttg tctatccttt tgaagctaag
     9901 caaacgcata aagggtgggc gcgccgaccc agctttcttg tacaaagtgg ttgataacag
     9961 cgggttaatt aacatctttt acccatacga tgttcctgac tatgcgggct atccctatga
    10021 cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg ctcagtgcag
    10081 cgcttagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
    10141 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
    10201 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
    10261 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
    10321 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttggttccgg aaccaattcg
    10381 taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    10441 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    10501 taggctgaat taggcgcgcc tatttctatt aattcccgat ctagtaacat agatgacacc
    10561 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
    10621 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
    10681 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
    10741 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc ggggaaattc
    10801 gggggatcga tcccgcgaac tgtggacgag aactgtgcca ccaagcgtaa ggccgttctc
    10861 tcgcatttgc cttgctaggc tcgcgcgagt tgctggctga ggcgttctcg aaatcagctc
    10921 ttgttcggtc ggcatctact ctattccttt gccctcggac gagtgctggg gcgtcggttt
    10981 ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg
    11041 atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc
    11101 gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca
    11161 atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg
    11221 aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg
    11281 acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg
    11341 ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg
    11401 gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg
    11461 gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa
    11521 tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc
    11581 tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg cagaacagcg
    11641 ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa
    11701 taggtcaggc tctcgctgaa tgccccaatg tcaagcactt ccggaatcgg gagcgcggcc
    11761 gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc
    11821 cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc
    11881 gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac
    11941 gtcgcggtga gttcaggctt tttcatatct tattgccccc cgggatcaga tctggattga
    12001 gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat
    12061 ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata
    12121 atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc tgagtggctc
    12181 cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg
    12241 ggggtcataa cgtgactccc ttaattctcc gctcatgatc ttgatcccct gcgccatcag
    12301 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
    12361 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat
    12421 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc
    12481 cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc
    12541 tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgaagct
    12601 gatccgtcga gattaataga aataaattca gcctaattcg gcgttaattc agtacattaa
    12661 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
    12721 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
    12781 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
    12841 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
    12901 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
    12961 gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
    13021 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
    13081 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
    13141 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
    13201 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg
    13261 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
    13321 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
    13381 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
    13441 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
    13501 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
    13561 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
    13621 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
    13681 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
    13741 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
    13801 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
    13861 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
    13921 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
    13981 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
    14041 gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt
    14101 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
    14161 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa
    14221 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    14281 cttc
//
