LOCUS       sequence-450402-        8485 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Transcription factor SOX13 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8485)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-SOX13-Brd 212
FEATURES             Location/Qualifiers
     source          1..8485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    4489..4892
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             5011..5244
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5316..5437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5353..5372)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5407..5426
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5477..5612
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5632..5651)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5633..5651)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5661..5678)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5661..5677)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5670..5692)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5819..6274
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5906..5925)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6116..6137
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6300..6404
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6405..6473,6474..7265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6623..6642)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7436..8024
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7925..7944
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8178..8195
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8312..8333
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8348..8353,8354..8371,8372..8378)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8386..8402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8391..8413
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8410..8426
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8410..8426
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8445..8465
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8447..8465
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctggctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacgccacc
     2401 atgtctatgc gctcacctat atcagcccag ctcgcgttgg acggcgtagg aacaatggtt
     2461 aattgtacga ttaaatcaga ggagaagaaa gaaccatgcc acgaggcacc tcagggtagt
     2521 gccacagctg ccgagcctca acccggagac cctgcgcgcg cgtcccaaga ttcagccgat
     2581 cctcaagctc ctgcacaagg aaactttagg gggtcctggg attgctcttc cccagagggc
     2641 aatggttcac ccgagccgaa acggcccggg gtgtcagaag ccgcatccgg tagtcaggag
     2701 aagttggact tcaatcgaaa cctgaaggaa gtcgtacctg ccatagagaa gctcctgagt
     2761 agtgattgga aggagcgatt tctgggcaga aattctatgg aagccaagga tgttaaggga
     2821 acacaagagt ctctcgccga gaaagagctg cagttgttgg ttatgatcca tcagctgagt
     2881 acactgcgag accagctttt gactgcccat tcagaacaaa aaaacatggc agcaatgctt
     2941 tttgaaaaac aacaacaaca aatggagctt gcaagacagc agcaagagca gatcgccaag
     3001 caacaacagc aactcattca acaacagcat aaaatcaatc tgctccaaca gcaaatccag
     3061 caggttaaca tgccgtatgt gatgataccc gcgttccctc cttcccacca gcccttgcct
     3121 gtcactccag atagccaatt ggctctgcct atacagccca taccctgtaa gcccgtagaa
     3181 tatcccctgc aattgctcca ttccccgcct gcccccgtcg taaaacggcc cggagccatg
     3241 gccactcacc atcctttgca ggagccttcc caacctctca atcttaccgc aaaacccaaa
     3301 gcgcccgaac ttcctaatac cagctctagc cccagcctga agatgagcag ctgcgtgcca
     3361 agacctcctt ctcacggtgg ccctaccaga gatctccaga gcagtcctcc tagcctgcct
     3421 ctgggatttc tcggagaagg cgacgccgtg acaaaggcca ttcaggacgc tagacagctg
     3481 ctccactctc actctggcgc cctggatgga agccccaaca cacccttcag aaaggacctg
     3541 atcagcctgg acagcagccc agccaaagag agacttgagg acggctgtgt gcaccctctg
     3601 gaagaggcca tgctgtcctg cgatatggac ggcagcagac acttccccga gagcagaaac
     3661 tccagccaca tcaagaggcc catgaacgcc tttatggtct gggccaaaga cgagcggcgg
     3721 aagatcctgc aagcctttcc tgacatgcac aacagctcca tcagcaagat cctgggctcc
     3781 agatggaagt ccatgaccaa tcaagagaaa cagccctact acgaggaaca ggcccggctg
     3841 agcagacagc acctggaaaa gtaccccgac tacaagtaca agcccagacc taagcggacc
     3901 tgcatcgtgg aaggcaagag actgagagtg ggcgagtaca aggccctgat gagaaccaga
     3961 cgccaggatg ccagacagag ctacgtgatc cctccacaag ctggccaggt gcagatgtct
     4021 agcagcgacg tgctgtaccc tagagccgcc ggaatgcctc tggctcagcc tctggtggaa
     4081 cactacgtgc ccagatcact ggaccccaac atgcccgtga tcgtgaacac ctgtagcctg
     4141 agagaggaag gcgagggcac cgacgataga cactctgtgg ctgacggcga gatgtacaga
     4201 tacagcgagg acgaggacag cgagggcgaa gagaagtctg atggcgaact ggtggtgctg
     4261 accgactgat aaggatcccc cgggctgcag gaattcgagc atcttaccgc catttattcc
     4321 catatttgtt ctgtttttct tgatttgggt atacatttaa atgttaataa aacaaaatgg
     4381 tggggcaatc atttacattt ttagggatat gtaattacta gttcaggtgt attgccacaa
     4441 gacaaacatg ttaagaaact ttcccgttat ttacgctctg ttcctgttaa tcaacctctg
     4501 gattacaaaa tttgtgaaag attgactgat attcttaact atgttgctcc ttttacgctg
     4561 tgtggatatg ctgctttaat gcctctgtat catgctattg cttcccgtac ggctttcgtt
     4621 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4681 cgtcaacgtg gcgtggtgtg ctctgtgttt gctgacgcaa cccccactgg ctggggcatt
     4741 gccaccacct gtcaactcct ttctgggact ttcgctttcc ccctcccgat cgccacggca
     4801 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctaggttgct gggcactgat
     4861 aattccgtgg tgttgtcggg gaagctgacg tcctttgcaa ttcgatatca agctgtacct
     4921 tctagatgta cagctaacca agcgcttcta gaccaatgac ttacaaggca gctgtagatc
     4981 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     5041 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5101 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5161 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5221 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     5281 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     5341 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5401 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     5461 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5521 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5581 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     5641 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     5701 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     5761 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     5821 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5881 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5941 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6001 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6061 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6121 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6181 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6241 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     6301 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6361 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6421 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6481 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6541 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6601 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6661 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6721 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6781 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6841 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6901 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6961 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7021 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7081 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7141 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7201 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7261 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7321 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7381 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7441 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7501 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7561 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7621 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7681 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7741 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7801 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7861 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7921 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7981 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8041 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8101 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8161 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8221 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     8281 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8341 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8401 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     8461 aaagggaaca aaagctggag ctgca
//
