LOCUS       sequence-450406-        7894 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Transcription factor SMAD7 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7894)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 7894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-SMAD7-Brd 211
FEATURES             Location/Qualifiers
     source          1..7894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    3898..4301
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             4420..4653
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4725..4846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4762..4781)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4816..4835
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4886..5021
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5041..5060)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5042..5060)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5070..5087)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5070..5086)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5079..5101)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5228..5683
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5315..5334)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5525..5546
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5709..5813
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5814..5882,5883..6674)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6032..6051)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6845..7433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7334..7353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7587..7604
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7721..7742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7757..7762,7763..7780,7781..7787)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7795..7811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7800..7822
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7819..7835
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7819..7835
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7854..7874
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7856..7874
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctggctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacgccacc
     2401 atgttccgga ccaagagatc tgccctcgtg cgtcggcttt ggagaagtag agcacctggc
     2461 ggcgaggacg aagaagaagg cgctggcggc ggaggtggtg gcggagaact tagaggcgaa
     2521 ggcgccacag atagcagagc acatggtgct ggcggaggcg gacctggtag agctggatgt
     2581 tgtctgggca aagccgtgcg gggagccaag ggacatcatc accctcatcc tccagctgcc
     2641 ggtgcaggcg cagcaggcgg agctgaagct gatctgaaag ccctgacaca cagcgtgctg
     2701 aagaagctga aagagagaca gctggaactg ctgctgcagg ccgttgaaag cagaggcgga
     2761 accagaacag cctgcctgtt gctgcctggc agactggatt gcagacttgg acctggtgct
     2821 cctgctggtg ctcagcctgc tcaacctcct agcagctact ctctgcctct gctgctgtgc
     2881 aaggtgttca gatggcccga cctgagacac agcagcgaag tgaagagact gtgctgctgc
     2941 gagagctacg gcaagatcaa ccccgaactc gtgtgctgca accctcacca cctgagcaga
     3001 ctgtgcgagc tggaatctcc tcctccacca tactctcgct accccatgga ctttctgaag
     3061 cccaccgatt gtcctgatgc tgttccttct tctgctgaaa ctggtggtac taattattta
     3121 gctcctggtg gtttatctga ttctcaatta ttattagaac ctggtgatcg ttctcattgg
     3181 tgtgttgttg cttattggga agaaaaaact cgtgttggtc gtttatattg tgttcaagaa
     3241 ccttctttag atatttttta tgatttacct caaggtaatg gtttttgttt aggtcaatta
     3301 aattctgata ataaatctca attagttcaa aaagttcgtt ctaaaattgg ttgtggtatt
     3361 caattaactc gtgaagttga tggtgtttgg gtttataatc gttcttctta tcctattttt
     3421 attaaatctg ctactttaga taatcctgat tctcgtactt tattagttca taaagttttt
     3481 cctggttttt ctattaaagc ttttgattat gaaaaagctt attctttaca acgtcctaat
     3541 gatcatgaat ttatgcaaca accttggact ggttttactg ttcaaatttc ttttgttaaa
     3601 ggttggggtc aatgttatac tcgtcaattt atttcttctt gtccttgttg gttagaagtt
     3661 atttttaatt ctcgttgata aggatccccc gggctgcagg aattcgagca tcttaccgcc
     3721 atttattccc atatttgttc tgtttttctt gatttgggta tacatttaaa tgttaataaa
     3781 acaaaatggt ggggcaatca tttacatttt tagggatatg taattactag ttcaggtgta
     3841 ttgccacaag acaaacatgt taagaaactt tcccgttatt tacgctctgt tcctgttaat
     3901 caacctctgg attacaaaat ttgtgaaaga ttgactgata ttcttaacta tgttgctcct
     3961 tttacgctgt gtggatatgc tgctttaatg cctctgtatc atgctattgc ttcccgtacg
     4021 gctttcgttt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4081 cccgttgtcc gtcaacgtgg cgtggtgtgc tctgtgtttg ctgacgcaac ccccactggc
     4141 tggggcattg ccaccacctg tcaactcctt tctgggactt tcgctttccc cctcccgatc
     4201 gccacggcag aactcatcgc cgcctgcctt gcccgctgct ggacaggggc taggttgctg
     4261 ggcactgata attccgtggt gttgtcgggg aagctgacgt cctttgcaat tcgatatcaa
     4321 gctgtacctt ctagatgtac agggtgcgta gcgcttctag accaatgact tacaaggcag
     4381 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     4441 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     4501 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4561 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4621 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     4681 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     4741 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4801 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     4861 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4921 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4981 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     5041 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     5101 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5161 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5221 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5281 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5341 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5401 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5461 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5521 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5581 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5641 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     5701 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5761 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5821 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5881 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5941 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6001 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6061 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6121 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6181 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6241 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6301 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6361 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6421 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6481 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6541 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6601 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6661 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6721 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6781 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6841 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6901 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6961 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7021 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7081 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7141 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7201 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7261 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7321 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7381 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7441 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7501 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7561 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7621 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     7681 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     7741 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     7801 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     7861 accctcacta aagggaacaa aagctggagc tgca
//
