LOCUS       sequence-450411-        9004 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Transcription factor SATB1 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9004)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 9004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-SATB1-Brd 210
FEATURES             Location/Qualifiers
     source          1..9004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2395..2404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5008..5411
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             5530..5763
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5835..5956
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5872..5891)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5926..5945
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5996..6131
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6151..6170)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6152..6170)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6180..6197)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6180..6196)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6189..6211)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6338..6793
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6425..6444)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6635..6656
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6819..6923
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6924..6992,6993..7784)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7142..7161)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7955..8543
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8444..8463
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8697..8714
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8831..8852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8867..8872,8873..8890,8891..8897)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8905..8921
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8910..8932
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8929..8945
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8929..8945
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8964..8984
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8966..8984
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctggctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacgccacc
     2401 atggaccacc tgaacgaggc cacacagggc aaagaacaca gcgagatgag caacaacgtg
     2461 tccgatccta agggccctcc tgccaagatc gccagactgg aacagaatgg cagccctctc
     2521 ggaagaggca gactgggatc tacaggcgcc aaaatgcagg gcgtgccact gaagcactct
     2581 ggccacctga tgaaaacaaa cctgcggaag ggcaccatgc tgcccgtgtt ctgtgtggtg
     2641 gaacactacg agaacgccat cgagtacgac tgcaaagagg aacacgccga gttcgtgctc
     2701 gtgcggaagg acatgctgtt caaccagctg atcgagatgg ccctgctgag cctgggctac
     2761 tctcatagct ctgccgctca ggccaagggc ctgatccaag tcggaaagtg gaaccccgtg
     2821 cctctgagct acgtgacaga tgcccctgat gccaccgtgg ccgatatgct ccaggatgtg
     2881 taccacgtcg tgaccctgaa gatccagctg cacagctgcc ccaagctgga agatctgcct
     2941 cctgaacagt ggtcccacac cacagtgcgg aacgccctga aggacctgct gaaggatatg
     3001 aaccagagca gcctggccaa agagtgccct ctgagccaga gcatgatcag cagcatcgtg
     3061 aacagcacct actacgccaa tgtgtccgcc gccaagtgcc aagagttcgg cagatggtac
     3121 aagcacttca aaaagaccaa ggacatgatg gtcgagatgg acagcctgag cgagctgtct
     3181 cagcaaggcg ccaaccacgt gaacttcgga cagcagcctg tgcctggaaa taccgccgaa
     3241 cagcctccat ctcctgctca gctttctcac ggctctcagc ctagtgtgcg gacccctctg
     3301 cctaatctgc accctggact ggtgtctacc cctatctctc cccagctggt caatcagcag
     3361 ctggttatgg cccagctcct gaaccagcag tacgccgtga atagactgct ggcacagcag
     3421 agtctgaatc agcaatacct gaatcaccct ccacctgtgt ccagatccat gaacaagccc
     3481 ctggaacagc aggtttccac caacaccgag gtgtccagcg agatctatca gtgggtccga
     3541 gatgaactga agagagccgg aatcagccag gccgtgtttg ccagagtggc cttcaataga
     3601 acccagggcc ttttgtctga gatactgcga aaagaagaag atccaaagac tgcaagccag
     3661 agcctcctgg tgaacctcag agctatgcag aactttctgc agctgccaga ggcggagaga
     3721 gatcgcatat accaagacga aagagagcga tctttgaacg ccgcgtccgc tatgggtccc
     3781 gctccgctta tctctacgcc gccatcaaga cccccacagg taaaaacagc aacgattgca
     3841 actgaaagga acgggaaacc tgaaaacaat acaatgaaca taaatgcaag catttatgat
     3901 gaaatacaac aggaaatgaa gagggcaaaa gtttcacagg ccttgtttgc aaaagttgcg
     3961 gcaacgaagt cacaaggttg gttgtgcgaa ctcctgcgat ggaaggaaga cccctctccg
     4021 gagaaccgga ctctctggga gaatctcagt atgatcagac gattcttgtc tcttcctcaa
     4081 cccgaacgag atgccattta tgaacaggaa tccaacgctg ttcatcacca tggtgacaga
     4141 ccaccacaca ttatacacgt accggcggag cagattcaat ctccatctcc cactacactt
     4201 ggtaagggtg aatctcgcgg cgtcttcctt cccgggttgc ctacacctgc accttggctg
     4261 ggggcggcgc cacagcaaca acaacaacag cagcaacaac agcagcagca acaacaagcg
     4321 cccccaccac cgcagccaca acagcagccc caaactgggc ctaggctgcc acccagacaa
     4381 cccaccgtcg cctctcccgc cgagtccgac gaagagaata gacagaaaac ccgacctcgc
     4441 accaagattt cagtggaagc cctcggcata ttgcaatcct tcatacaaga cgtcggactc
     4501 tatccggacg aagaagcgat ccaaacactc agtgcacaac tcgatctgcc aaaatacacg
     4561 ataataaaat tctttcaaaa tcagcggtac tatttgaagc accatggcaa gttgaaggac
     4621 aattcaggcc ttgaagtgga cgtagcggaa tacaaagagg aagagcttct gaaagacttg
     4681 gaggaaagcg tgcaagacaa gaatacaaac acccttttct cagtgaagct tgaggaggaa
     4741 cttagtgtcg aaggcaacac agatataaat acggacctca aggactgata aggatccccc
     4801 gggctgcagg aattcgagca tcttaccgcc atttattccc atatttgttc tgtttttctt
     4861 gatttgggta tacatttaaa tgttaataaa acaaaatggt ggggcaatca tttacatttt
     4921 tagggatatg taattactag ttcaggtgta ttgccacaag acaaacatgt taagaaactt
     4981 tcccgttatt tacgctctgt tcctgttaat caacctctgg attacaaaat ttgtgaaaga
     5041 ttgactgata ttcttaacta tgttgctcct tttacgctgt gtggatatgc tgctttaatg
     5101 cctctgtatc atgctattgc ttcccgtacg gctttcgttt tctcctcctt gtataaatcc
     5161 tggttgctgt ctctttatga ggagttgtgg cccgttgtcc gtcaacgtgg cgtggtgtgc
     5221 tctgtgtttg ctgacgcaac ccccactggc tggggcattg ccaccacctg tcaactcctt
     5281 tctgggactt tcgctttccc cctcccgatc gccacggcag aactcatcgc cgcctgcctt
     5341 gcccgctgct ggacaggggc taggttgctg ggcactgata attccgtggt gttgtcgggg
     5401 aagctgacgt cctttgcaat tcgatatcaa gctgtacctt ctagatgtac aggttcataa
     5461 gcgcttctag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5521 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     5581 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5641 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5701 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5761 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     5821 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5881 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5941 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     6001 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6061 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6121 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     6181 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     6241 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     6301 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     6361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6481 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6541 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6601 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6661 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6721 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6781 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6841 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6901 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6961 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7021 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7081 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7141 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7201 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7261 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7321 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7381 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7441 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7501 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7561 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7621 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7681 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7741 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7801 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7861 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7921 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7981 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8041 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8101 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8161 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8221 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8281 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8341 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8401 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8461 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8521 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8581 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8641 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8701 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8761 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8821 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8881 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     8941 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     9001 tgca
//
