LOCUS       sequence-450413-        8380 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Transcription factor REL with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8380)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 8380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-REL-Brd 205
FEATURES             Location/Qualifiers
     source          1..8380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2395..2404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4384..4787
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             4906..5139
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5211..5332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5248..5267)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5302..5321
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5372..5507
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5527..5546)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5528..5546)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5556..5573)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5556..5572)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5565..5587)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5714..6169
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5801..5820)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6011..6032
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6195..6299
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6300..6368,6369..7160)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6518..6537)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7331..7919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7820..7839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8073..8090
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8207..8228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8243..8248,8249..8266,8267..8273)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8281..8297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8286..8308
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8305..8321
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8305..8321
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8340..8360
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8342..8360
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctggctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacgccacc
     2401 atggccagcg gcgcctacaa tccctacatc gagatcatcg agcagccccg gcagcggggc
     2461 atgagattca gatacaagtg cgagggaaga agcgccggct ctattcctgg cgagcacagc
     2521 accgacaaca accggacata ccccagcatc cagatcatga actactacgg caagggcaaa
     2581 gtgcggatca ccctggtcac caagaacgac ccctacaagc cccatcctca cgacctcgtg
     2641 ggcaaagatt gccgcgacgg ctattacgag gccgagttcg gacaagagag aaggcccctg
     2701 ttcttccaga acctgggcat cagatgcgtg aagaagaaag aagtcaaaga ggccatcatc
     2761 acccggatca aggccggcat caaccccttc aatgtgcccg agaagcagct gaacgacatc
     2821 gaggactgcg acctgaatgt cgtgcggctg tgcttccagg tgttcctgcc tgatgagcac
     2881 ggcaacctga caacagctct gcctcctgtg gtgtctaacc ccatctacga caacagagcc
     2941 cctaacaccg ccgagctgcg gatctgtaga gtgaacaaga actgcggcag cgtcagaggc
     3001 ggcgacgaga tctttctgct gtgcgacaag gtgcagaaag acgatatcga agtccgcttc
     3061 gtgctgaacg attgggaagc caagggcatc ttcagccagg ccgatgtgca tagacaggtg
     3121 gccatcgtgt tcaagacccc tccatactgc aaggccatca ccgagcctgt gaccgtgaag
     3181 atgcagctga gaaggcctag cgaccaagag gtgtccgaga gcatggactt ccgctacctg
     3241 cctgacgaga aggacaccta cggcaacaag gccaagaagc agaaaaccac actgctgttc
     3301 cagaagctgt gccaggacca cgtgaacttc cccgagaggc ctagacctgg actgctgggc
     3361 tctatcggcg agggcagata cttcaagaaa gagcccaacc tgttcagcca cgacgccgtt
     3421 gtcagagaaa tgcctacagg cgtgtccagc caggctgaga gctactaccc atctcctgga
     3481 cctatcagca gcggcctgtc tcaccatgcc tctatggctc ctctgcctag cagcagctgg
     3541 tcctctgtgg ctcaccctac acctagaagc ggcaacacca atcctctgag cagcttcagc
     3601 acccggacac tgccaagcaa cagccagggc atccctccat ttctgagaat ccccgtgggc
     3661 aacgacctga acgccagcaa tgcctgcatc tacaacaacg ccgatgacat cgtcggcatg
     3721 gaagccagct ctatgcctag cgccgatctg tacggcatca gcgaccccaa catgctgagc
     3781 aactgctccg tgaacatgat gaccaccagc agcgacagca tgggcgagac agacaaccct
     3841 agactgctga gcatgaacct ggaaaacccc agctgcaaca gcgtgctgga ccccagagat
     3901 ctgagacagc tgcaccagat gtccagcagc tccatgtctg ccggcgctaa cagcaacacc
     3961 accgtgtttg tgtctcagag cgacgccttc gagggcagcg atttcagctg cgccgacaac
     4021 agcatgatca acgagagcgg ccccagcaac agcacaaacc ctaatagcca cggcttcgtg
     4081 caggacagcc agtactctgg catcggcagc atgcagaacg agcagctgag cgatagcttc
     4141 ccctacgagt tctttcaggt gtagtaagga tcccccgggc tgcaggaatt cgagcatctt
     4201 accgccattt attcccatat ttgttctgtt tttcttgatt tgggtataca tttaaatgtt
     4261 aataaaacaa aatggtgggg caatcattta catttttagg gatatgtaat tactagttca
     4321 ggtgtattgc cacaagacaa acatgttaag aaactttccc gttatttacg ctctgttcct
     4381 gttaatcaac ctctggatta caaaatttgt gaaagattga ctgatattct taactatgtt
     4441 gctcctttta cgctgtgtgg atatgctgct ttaatgcctc tgtatcatgc tattgcttcc
     4501 cgtacggctt tcgttttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4561 ttgtggcccg ttgtccgtca acgtggcgtg gtgtgctctg tgtttgctga cgcaaccccc
     4621 actggctggg gcattgccac cacctgtcaa ctcctttctg ggactttcgc tttccccctc
     4681 ccgatcgcca cggcagaact catcgccgcc tgccttgccc gctgctggac aggggctagg
     4741 ttgctgggca ctgataattc cgtggtgttg tcggggaagc tgacgtcctt tgcaattcga
     4801 tatcaagctg taccttctag atgtacatta tacttagcgc ttctagacca atgacttaca
     4861 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     4921 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     4981 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5041 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     5101 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     5161 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     5221 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5281 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5341 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5401 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5461 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     5521 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     5581 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     5641 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     5701 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5761 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5821 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5881 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5941 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6001 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6061 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6121 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     6181 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6241 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6301 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6361 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6421 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6481 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6541 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6601 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6661 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6721 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6781 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6841 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6901 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6961 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7021 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7081 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7141 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7201 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7261 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7321 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7381 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7441 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7501 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7561 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7621 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7681 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7741 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7801 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7861 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7921 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7981 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8041 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8101 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     8161 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     8221 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     8281 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     8341 caattaaccc tcactaaagg gaacaaaagc tggagctgca
//
