LOCUS       sequence-450442-        7973 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Transcription factor ZNF506 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7973)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 7973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-ZNF506-Brd 144
FEATURES             Location/Qualifiers
     source          1..7973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             96..329
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    401..522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(438..457)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     492..511
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      562..697
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(717..736)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(718..736)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(746..763)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(746..762)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(755..777)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      904..1359
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(991..1010)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1201..1222
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1385..1489
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1490..1558,1559..2350)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1708..1727)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2521..3109
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3010..3029
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3263..3280
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3397..3418
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3433..3438,3439..3456,3457..3463)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3471..3487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3476..3498
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3495..3511
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3495..3511
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3530..3550
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3532..3550
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3578..3804
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3805..3985
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4032..4157
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4650..4883
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5068..5112
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5379..5496
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5530..5936
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    7547..7950
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
ORIGIN
        1 taccttctag atgtacatgc gcgctagcgc ttctagacca atgacttaca aggcagctgt
       61 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
      121 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
      181 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      241 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
      301 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
      361 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
      421 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      481 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
      541 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      601 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      661 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
      721 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      781 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      841 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      901 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      961 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     1021 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     1081 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     1141 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     1201 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     1261 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     1321 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     1381 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1441 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     1501 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     1561 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     1621 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     1681 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     1741 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     1801 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     1861 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     1921 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1981 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     2041 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     2101 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2161 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2221 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2281 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2341 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2401 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2461 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2521 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2581 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2641 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2701 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2761 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2821 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2881 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2941 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3001 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3061 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3121 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3181 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3241 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     3301 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     3361 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     3421 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     3481 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     3541 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     3601 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     3661 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     3721 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     3781 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     3841 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3901 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3961 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     4021 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     4081 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     4141 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     4201 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     4261 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     4321 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     4381 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     4441 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     4501 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     4561 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     4621 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     4681 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     4741 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     4801 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     4861 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     4921 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     4981 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     5041 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     5101 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     5161 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     5221 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     5281 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     5341 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     5401 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     5461 gaattacaaa aacaaattac aaaaattcaa aattttatcg atcacgagac tagcctcgag
     5521 ctggctgcag taacgccatt ttgcaaggca tggaaaaata ccaaaccaag aatagagaag
     5581 ttcagatcaa gggcgggtac atgaaaatag ctaacgttgg gccaaacagg atatctgcgg
     5641 tgagcagttt cggccccggc ccggggccaa gaacagatgg tcaccgcagt ttcggccccg
     5701 gcccgaggcc aagaacagat ggtccccaga tatggcccaa ccctcagcag tttcttaaga
     5761 cccatcagat gtttccaggc tcccccaagg acctgaaatg accctgcgcc ttatttgaat
     5821 taaccaatca gcctgcttct cgcttctgtt cgcgcgcttc tgcttcccga gctctataaa
     5881 agagctcaca acccctcact cggcgcgcca gtcctccgac agactgagtc ggccggtcga
     5941 atcaagctta tcgataccgt cgacggatcc gctagcccgc ggttcgaaat gggaccattg
     6001 caatttagag atgtggccat agaattctct ctggaggagt ggcattgcct ggacgctgca
     6061 cagcggaatc tatataggga tgtgatgtta gagaactaca gaaacctgat cttccttggt
     6121 attgttgtct ctaaaccaaa cctgatcacc tgtctggagc aaggaaaaaa acctttaact
     6181 atgaagaggc atgagatgat tgccaaaccc ccagttatgt attctcattt tgcccaagac
     6241 ctttggtcag agcagagcat aaaagattct ttccaaaaag tgatactaag aagatatgaa
     6301 aaatgtagac atgacaattt acagttaaaa aaaggctgtg aaagtgtaga tgagtgtcca
     6361 gtgcacaaaa gaggttataa tggacttaaa caatgtttgg caactaccca gagaaaaata
     6421 tttcaatgtg atgaatatgt gaaattcttg cataaatttt caaattcaaa caaacataag
     6481 ataagagata ctggaaaaaa atcttttaaa tgtatagaat atgggaaaac ttttaaccag
     6541 tcttcaaccc gtactacata taagaaaatt gatgctggag agaaacgcta taaatgtgaa
     6601 gaatgtggta aagcctataa gcagtcctca caccttacta cacataagaa aattcatact
     6661 ggagagaaac cctacaaatg tgaagaatgt ggcaaagcct ataagcagtc ctgtaacctt
     6721 actacacata agataattca tactggagag aaaccctaca gatgtagaga atgtggcaaa
     6781 gcttttaacc accctgcaac ccttttttca cataagaaaa ttcatactgg agagaaacca
     6841 tacaagtgtg ataaatgtgg caaagccttt atttcatcct caacccttac taaacatgag
     6901 ataattcata ctggagagaa accctacaaa tgtgaggaat gtggcaaagc ttttaaccgt
     6961 tcctcaaacc ttactaaaca taagagaatt catactggag atgtacccta caaatgtgac
     7021 gaatgtggca aaacctttac ctggtactca agcctctcta aacataagag agctcatact
     7081 ggagagaaac cctacaagtg tgaagaatgt ggcaaagcct ttactgcatt ctcaactcta
     7141 actgaacata agataattca tactggagag aaaccgtaca aatgtgaaga atgtggcaaa
     7201 gcttttaact ggtcctcagc ccttaataaa cataagaaaa ttcatattag acagaaaccc
     7261 tgcatagtga agaatgtgga aaatctttta aatgttcctc aacccttaat aagcataaga
     7321 tagaccggtc ggatcccccg ggctgcagga attcgagcat cttaccgcca tttattccca
     7381 tatttgttct gtttttcttg atttgggtat acatttaaat gttaataaaa caaaatggtg
     7441 gggcaatcat ttacattttt agggatatgt aattactagt tcaggtgtat tgccacaaga
     7501 caaacatgtt aagaaacttt cccgttattt acgctctgtt cctgttaatc aacctctgga
     7561 ttacaaaatt tgtgaaagat tgactgatat tcttaactat gttgctcctt ttacgctgtg
     7621 tggatatgct gctttaatgc ctctgtatca tgctattgct tcccgtacgg ctttcgtttt
     7681 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtccg
     7741 tcaacgtggc gtggtgtgct ctgtgtttgc tgacgcaacc cccactggct ggggcattgc
     7801 caccacctgt caactccttt ctgggacttt cgctttcccc ctcccgatcg ccacggcaga
     7861 actcatcgcc gcctgccttg cccgctgctg gacaggggct aggttgctgg gcactgataa
     7921 ttccgtggtg ttgtcgggga agctgacgtc ctttgcaatt cgatatcaag ctg
//
