LOCUS       sequence-450443-        8288 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Transcription factor SP110 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8288)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 8288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-SP110-Brd 146
FEATURES             Location/Qualifiers
     source          1..8288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    136..261
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             308..488
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(489..715)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(743..763)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(743..761)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(782..798)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(782..798)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(795..817)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    806..822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(830..836,837..854,855..860))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    875..896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1013..1030)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1184..1772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1264..1283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1943..2734,2735..2803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2566..2585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2804..2908)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2934..3389)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3071..3092)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3283..3302
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3516..3538
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3530..3547
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3531..3547
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3557..3576
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3557..3575
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3596..3731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3771..3892
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3782..3801)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3836..3855
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3964..4197
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4316..4719
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     promoter        complement(6645..7051)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    7085..7202
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7469..7513)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7698..7931
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa
       61 tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct
      121 cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa
      181 aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag
      241 ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg
      301 gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
      361 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc
      421 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
      481 agagacccgt ttatgtatcg agctaggcac ttaaatacaa tatctctgca atgcggcaat
      541 tcagtggttc gtccaatcca tgtcagaccc gtctgttgcc ttcctaataa ggcacgatcg
      601 taccacctta cttccaccaa tcggcatgca cggtgctttt tctctccttg taaggcatgt
      661 tgctaactca tcgttaccat gttgcaagac tacaagagta ttgcataaga ctacattaag
      721 cttgcagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
      781 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      841 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      901 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      961 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1021 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1081 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1141 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1201 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1261 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1321 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1381 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1441 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1501 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1561 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1621 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1681 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1741 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1801 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1861 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1921 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     1981 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2041 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2101 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2161 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2221 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2281 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2341 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2401 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2461 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2521 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2581 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2641 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2701 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2761 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     2821 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     2881 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     2941 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     3001 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     3061 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     3121 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3181 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     3241 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     3301 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     3361 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     3421 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     3481 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     3541 ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtacgt ccctaggcct
     3601 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
     3661 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     3721 aggggcggga tgggcggagt taggggcggg atagctagag ccagacatga taagatacat
     3781 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     3841 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttcctctcac
     3901 tctctgatat tcatttcttt gcaagttata aatactgaat aataagatga catgaactac
     3961 tactgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     4021 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     4081 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     4141 acccagtaca agcaaaaagc agatcttgtc ttcgttggga gtgaattagc ccttccagtc
     4201 cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctag
     4261 aagcgcttaa gaatatgtac atctagaagg tacagcttga tatcgaattg caaaggacgt
     4321 cagcttcccc gacaacacca cggaattatc agtgcccagc aacctagccc ctgtccagca
     4381 gcgggcaagg caggcggcga tgagttctgc cgtggcgatc gggaggggga aagcgaaagt
     4441 cccagaaagg agttgacagg tggtggcaat gccccagcca gtgggggttg cgtcagcaaa
     4501 cacagagcac accacgccac gttgacggac aacgggccac aactcctcat aaagagacag
     4561 caaccaggat ttatacaagg aggagaaaac gaaagccgta cgggaagcaa tagcatgata
     4621 cagaggcatt aaagcagcat atccacacag cgtaaaagga gcaacatagt taagaatatc
     4681 agtcaatctt tcacaaattt tgtaatccag aggttgatta acaggaacag agcgtaaata
     4741 acgggaaagt ttcttaacat gtttgtcttg tggcaataca cctgaactag taattacata
     4801 tccctaaaaa tgtaaatgat tgccccacca ttttgtttta ttaacattta aatgtatacc
     4861 caaatcaaga aaaacagaac aaatatggga ataaatggcg gtaagatgct cgaattcctg
     4921 cagcccgggg gatccgaccg gttcacttgc tatttaactc tctcttgaga tttattcttg
     4981 gaggacagag caaaagtcca ctcttcagca gctctcctag ggtcattcct tcacaacgta
     5041 tattccgttt ccagttcttt gcgttccttc cttttccttc gacttcaaat tcatttggtg
     5101 ttaaccaagt tccatcctca ttccgaatgc acttcactga ggatccgtgt ttcattttct
     5161 tcttatataa aatccctttc gcctcaccac aggtcacggg gagcttagaa cagtgaaaat
     5221 ccacagtgtc acttttgggt tttcctcttc ggtgaatatt tttctgaaat ctcctttttg
     5281 agcttgaaca gatatctttc tcctttttct tttctttcaa tctggacttt cgggcacatt
     5341 tagttcttgc cttttggacc ctcatcatga cctctgagtt ccaggttgag tcgtctttcc
     5401 tttgagtcac cttatccacc acttggagct tctcttggat gccatgccca ggtgaggctg
     5461 ccccttgtgt gacccttcgt ggtgtactag gcgtcttctg ggacctcttt ccttcattca
     5521 tttctgaagt gccatcaatg atctcctctg atctcgactt tcgggcacat tcagttctcg
     5581 ccttttgggc ccttgtctct accgtggagt tacaagttga gtcatctttc ttttgaggaa
     5641 cctgatccac ccttttgagc ttcttttgga ttccgtgtct agatgaggct gtccctcctg
     5701 ggaggctttt tttcttatgt ctcctttttg gagttgacca gatacatctt tttcttttct
     5761 ttcctttctt gtctgaaggt gtgctggaca cctcctgggg ctcttctggg tcatttggtt
     5821 ctggagaatt atctcttatc tctggcatag agcccaaggg agagtggggc atctcttgag
     5881 ggtcttcttt atctcttatt tgggggatca ggttgtcact ggccacttgc acagtgctag
     5941 tgaggaggct gggcatctct tctgagtctt cttccgcatt cattttggat gttaacttgt
     6001 cattggtcac tgaagtgctt cttccttcct ggatgagtgc agggagaggc aggacagggt
     6061 cagatgggct gggcgactca ctcaggatct catcgctttg ctgggaggat gttccaggct
     6121 cactgactct tggcgcacag ggtgaacagc ttggttgagg gggttgtggt gggggcagcg
     6181 ccagtggggt atggagggag cttccttctg ctaggccagt tggggcttca agtaggattg
     6241 gtgtgtctct gctctgccat tcataggaag caccaacacg tttgaagctt ctgtaaatcg
     6301 tcaccagatt gggatattca cgcaggttaa tttgactgaa caatgtcacc agaagagaca
     6361 ggttaaaagt cctctccagt tgggtgagaa tgttgtgcac cactctggat acagggatca
     6421 aatttctaca ggcttccaga gattccatgt acattctctt agtgatgatg gagttgtcta
     6481 ggaggccttc aaagaaggga aatggcttgt gtatggcata ggcgatcccc agcttctggt
     6541 gcatgaagtg ctgaaaaaga gcctcttcca tggctcttgt catggtgaac atttcgaacc
     6601 gcgggctagc ggatccgtcg acggtatcga taagcttgat tcgaccggcc gactcagtct
     6661 gtcggaggac tggcgcgccg agtgaggggt tgtgagctct tttatagagc tcgggaagca
     6721 gaagcgcgcg aacagaagcg agaagcaggc tgattggtta attcaaataa ggcgcagggt
     6781 catttcaggt ccttggggga gcctggaaac atctgatggg tcttaagaaa ctgctgaggg
     6841 ttgggccata tctggggacc atctgttctt ggcctcgggc cggggccgaa actgcggtga
     6901 ccatctgttc ttggccccgg gccggggccg aaactgctca ccgcagatat cctgtttggc
     6961 ccaacgttag ctattttcat gtacccgccc ttgatctgaa cttctctatt cttggtttgg
     7021 tatttttcca tgccttgcaa aatggcgtta ctgcagccag ctcgaggcta gtctcgtgat
     7081 cgataaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg
     7141 ctattatgtc tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa
     7201 aagttaaccg ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct
     7261 ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg
     7321 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
     7381 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
     7441 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
     7501 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
     7561 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
     7621 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
     7681 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
     7741 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
     7801 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
     7861 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
     7921 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg
     7981 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc
     8041 aattgtccct catatctcct cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg
     8101 gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc
     8161 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct
     8221 tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat
     8281 gtttctaa
//
