LOCUS       sequence-450583-        8498 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful 
            for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 
            nM, n = 0.53).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Fiedler BL, Van Buskirk S, Carter KP, Qin Y, Carpenter MC, Palmer 
            AE, Jimenez R
  TITLE     Droplet Microfluidic Flow Cytometer For Sorting On Transient 
            Cellular Responses Of Genetically-Encoded Sensors.
  JOURNAL   Anal Chem. 2017 Jan 3;89(1):711-719. doi: 
            10.1021/acs.analchem.6b03235. Epub 2016 Dec 13.
  PUBMED    27959493
REFERENCE   2  (bases 1 to 8498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.analchem.6b03235"; journalName: "Anal Chem"; date: 
            "2017-01-3- 3"; volume: "89"; issue: "1"; pages: "711-719"
COMMENT     Sequence Label: PB-NES-ZapCV2
FEATURES             Location/Qualifiers
     source          1..8498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..26
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(245..349)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(375..830)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(512..533)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     724..743
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     957..979
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     971..988
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     972..988
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     998..1017
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        998..1016
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   1046..1080
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1383..1762
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1764..2039
                     /label=chicken beta-actin promoter
     intron          2040..3056
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2980..3002
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3064..3083
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3141..3167
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(3177..3179,3180..3182,3183..3695)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             4083..4817
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     misc_feature    4934..5483
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5078..5095)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5305..5324
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5954..5971)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5960..6184
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6231..6247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6255..6271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6279..6285,6286..6303,6304..6309))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6324..6345
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6579..6641
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6682..6702)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6682..6700)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6721..6737)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6745..6761
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6769..6775,6776..6793,6794..6799))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6814..6835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6952..6969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7123..7711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7203..7222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7882..8498,1..175,176..244))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
       61 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
      121 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
      181 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
      241 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      301 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
      361 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
      421 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
      481 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
      541 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
      601 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
      661 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
      721 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
      781 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
      841 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      901 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
      961 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
     1021 attggggcgc gccattctag attaacccta gaaagatagt ctgcgtaaaa ttgacgcatg
     1081 cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg tgcatttagg
     1141 acatctcagt cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg taagtgtcac
     1201 tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct tttacgtgac
     1261 ttttaagatt taactcatac gataattata ttgttatttc atgttctact tacgtgataa
     1321 cttattatat atatattttc ttgttataga tatcaactag aatgctagca tgggcccatc
     1381 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     1441 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     1501 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     1561 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     1621 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     1681 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     1741 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     1801 ctcccccccc tccccccccc caattttgta tttatttttt ttttaatttt tttgggcggg
     1861 gggggggggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
     1921 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
     1981 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
     2041 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
     2101 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     2161 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
     2221 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
     2281 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
     2341 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     2401 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     2461 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     2521 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     2581 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     2641 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     2701 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     2761 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     2821 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     2881 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     2941 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     3001 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     3061 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc aacttaagct
     3121 tggtaccgag catgcttcaa cttcctcctc ttgaacgtct tactctttcg gatcccatgg
     3181 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3241 acgtaaacgg ccacaggttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3301 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3361 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3421 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgtacc atcttcttca
     3481 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     3541 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     3601 tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
     3661 tcaaggccca cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     3721 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     3781 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3841 tggagttcgt gaccgccgcc cgcatgcata aaaacaatga cttaaaacac aaatggaaag
     3901 aatgtcctga gtcttgtagc tcactatttg acctacaaag acatcttttg aaggatcatg
     3961 tctctcaaga tttcaaacac cctatggaac cattagctca taattgggag gactgtgatt
     4021 tccttggaga tgatacatgt tccatagtga accatattaa ttgtcaacat ggtatcgagc
     4081 tcatggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     4141 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     4201 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4261 tcggcatgga cgagctgtac aagggtggca gcggtggcat ggtgagcaag ggcgaggagc
     4321 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     4381 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
     4441 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
     4501 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     4561 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     4621 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     4681 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     4741 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     4801 tccgccacaa catcgagtaa gaatcctgca gatatccagc acagtgggtc gacgggccgc
     4861 ggtaacaatt gttaactaac ttaagctagc aacggtttcc ctctagcggg atcaattccg
     4921 cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     4981 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     5041 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     5101 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     5161 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     5221 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     5281 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     5341 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     5401 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     5461 ttcctttgaa aaacacgata ataccatggt catgaaaaca tttaacattt ctcaacaaga
     5521 tctagaatta gtagaagtag cgacagagaa gattacaatg ctttatgagg ataataaaca
     5581 tcatgtggga gcggcaattc gtacgaaaac aggagaaatc atttcggcag tacatattga
     5641 agcgtatata ggacgagtaa ctgtttgtgc agaagccatt gcgattggta gtgcagtttc
     5701 gaatggacaa aaggattttg acacgattgt agctgttaga cacccttatt ctgacgaagt
     5761 agatagaagt attcgagtgg taagtccttg tggtatgtgt agggagttga tttcagacta
     5821 tgcaccagat tgttttgtgt taatagaaat gaatggcaag ttagtcaaaa ctacgattga
     5881 agaactcatt ccactcaaat atacccgaaa ttaaaagtcg agatgcatcg atgatctaga
     5941 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     6001 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     6061 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     6121 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     6181 atggcttctg aggcggaaag aacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     6241 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6301 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6361 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tcttaaaagt tttgttactt
     6421 tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat aaataaattg
     6481 tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa
     6541 attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt ttacgcatga
     6601 ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaatctag tatacgcgtt
     6661 taattaatcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     6721 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6781 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6841 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6901 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7561 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7801 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7861 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7921 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7981 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8041 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8101 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8161 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8221 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8281 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8341 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8401 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8461 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaa
//
