LOCUS       sequence-45194-e         878 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Liao B, Bao X, Liu L, Feng S, Zovoilis A, Liu W, Xue Y, Cai J, Guo 
            X, Qin B, Zhang R, Wu J, Lai L, Teng M, Niu L, Zhang B, Esteban MA, 
            Pei D
  TITLE     MicroRNA cluster 302-367 enhances somatic cell reprogramming by 
            accelerating a mesenchymal-to-epithelial transition.
  JOURNAL   J Biol Chem. 2011 May 13. 286(19):17359-64.
  PUBMED    21454525
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 May 13. 286(19):17359-64."
COMMENT     Sequence Label: pMXs-B6
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             296..319
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    326..359
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tggaccatcc tctagactgc cggatctagc tagttaatta aggatccgtt aacaagcttc
       61 acttctagta ctcctggatc aaccaaggtc ttgttgaagc aactacagcg caaggacatt
      121 gctacttacg attagttttg cagatttgca gttcagcgta tatgtgaata tatggctgtg
      181 caaatccatg caaaactgat tgtgggaatg tgtacctttc tgcatcgtaa tggacacctt
      241 tatgcacatc ttcagcatcc atgcccattc atccacagaa ttcgtataca ctagtgacta
      301 caaggacgac gatgacaagt gataaataac ttcgtataat gtatgctata cgaagttatt
      361 ctagtattta aatgccctcg agctgactgc ggccgccagc acagtggtcg acgataaaat
      421 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
      481 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag
      541 agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat
      601 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg
      661 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
      721 gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt
      781 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
      841 gcttctgttc gcgcgcttct gctccccgag ctcaataa
//
