LOCUS       sequence-452317-       10979 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Bacterial expression of SynPspA with C-terminal YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Junglas B, Huber ST, Heidler T, Schlosser L, Mann D, Hennig R, 
            Clarke M, Hellmann N, Schneider D, Sachse C
  TITLE     PspA adopts an ESCRT-III-like fold and remodels bacterial membranes.
  JOURNAL   Cell. 2021 Jul 8;184(14):3674-3688.e18. doi: 
            10.1016/j.cell.2021.05.042. Epub 2021 Jun 23.
  PUBMED    34166616
REFERENCE   2  (bases 1 to 10979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.05.042"; journalName: "Cell"; date: "2021-07-8-
            8"; volume: "184"; issue: "14"; pages: "3674-3688.e18"
COMMENT     Sequence Label: pBAD_SynPspA
FEATURES             Location/Qualifiers
     source          1..10979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    396..521
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1014..1247
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1432..1476
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1859..3037
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2090..3028
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2985..3005
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3085..3202
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3300..3868
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3462..3479)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3689..3708
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3901..4500
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3901..3920)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4397..4417
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4524..5112
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4577..4597)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4995..5006)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5256..5289)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5309..5489
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5514..5532)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5515..5532)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5822..5841)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6055..6074)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6174..6196
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6234..6252)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6320..6424
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6425..6493,6494..7285)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6643..6662)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7456..8044
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7945..7964
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8198..8215
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8285..8305)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8290..8619
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8470..8605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9753..9818
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9948..9968
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10393..10527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10430..10449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10484..10503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10695..10979,1..349)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
       61 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
      121 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
      181 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      241 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      301 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      361 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      421 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      481 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      541 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      601 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      661 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      721 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
      781 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
      841 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
      901 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
      961 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1021 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1081 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1141 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1201 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1261 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1321 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1381 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1441 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1501 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1561 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1621 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1681 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca
     1741 cgagactagc ctcgaccatc gatgtcgacg ataagctttg caaagatgga taaagtttta
     1801 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg
     1861 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     1921 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     1981 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     2041 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     2101 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     2161 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     2221 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
     2281 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     2341 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     2401 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
     2461 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     2521 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     2581 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     2641 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     2701 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     2761 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     2821 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     2881 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     2941 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     3001 ggttcaaagt ttttttcttc catttcaggt gtcgtgagga atttcgacat ttaaatttaa
     3061 ttaatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
     3121 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     3181 aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgaggttt aaacctgcag
     3241 gcgcgccgga tcccccgggg cgatcgcacg cgtactagta aactacgggc tgcaggaatt
     3301 ccgccccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     3361 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     3421 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     3481 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     3541 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     3601 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     3661 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     3721 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     3781 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     3841 ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccacaaggag acgaccttcc
     3901 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
     3961 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac
     4021 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     4081 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     4141 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     4201 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     4261 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     4321 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     4381 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     4441 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     4501 gtcgacctcg agggaattcc gataatcaac ctctggatta caaaatttgt gaaagattga
     4561 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4621 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4681 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4741 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4801 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4861 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     4921 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4981 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     5041 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     5101 ccgcctcccc gcatcgggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
     5161 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     5221 caacgaagac aagatgggat caattcacca tgggaataac ttcgtatagc atacattata
     5281 cgaagttatg ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5341 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5401 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5461 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
     5521 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     5581 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
     5641 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
     5701 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
     5761 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     5821 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     5881 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     5941 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     6001 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     6061 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     6121 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     6181 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     6241 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6301 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6361 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6421 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6481 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6541 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6601 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6661 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6721 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6781 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6841 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6901 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6961 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7021 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7081 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7141 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7201 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7261 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7321 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7381 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7441 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7501 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7561 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7621 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7681 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7741 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7801 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7861 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7921 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7981 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8041 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8101 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8161 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8221 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     8281 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     8341 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8401 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     8461 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8521 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8581 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
     8641 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     8701 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     8761 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     8821 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     8881 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
     8941 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     9001 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     9061 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     9121 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     9181 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     9241 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     9301 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     9361 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     9421 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     9481 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     9541 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     9601 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     9661 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     9721 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     9781 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     9841 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     9901 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     9961 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
    10021 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
    10081 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
    10141 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
    10201 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
    10261 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
    10321 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    10381 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    10441 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10501 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    10561 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    10621 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    10681 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
    10741 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
    10801 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
    10861 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
    10921 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttc
//
