LOCUS       sequence-453368-        5123 bp    DNA     circular SYN 15-APR-2025
DEFINITION  AiE0140h_3xC2 is an enhancer sequence, designed to drive 
            AAV-mediated transgene expression in striatal Pvalb interneurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Hunker AC, Wirthlin ME, Gill G, Johansen NJ, Hooper M, Omstead V, 
            Taskin N, Weed N, Vargas S, Bendrick JL, Gore B, Ben-Simon Y, Bishaw
            Y, Opitz-Araya X, Martinez RA, Way S, Thyagarajan B, Lerma MN, Laird
            W, Sven O, Sanchez REA, Alexander JR, Amaya A, Amster A, Ayala A, 
            Baker PM, Barcelli T, Barta S, Bertagnolli D, Bielstein C, 
            Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, Casper T, 
            Chakka AB, Chakrabarty R, Clark M, Colbert K, Daniel S, Dawe T, 
            Departee M, DiValentin P, Donadio NP, Dotson NI, Dwivedi D, Egdorf 
            T, Fliss T, Gary A, Goldy J, Grasso C, Groce EL, Gudsnuk K, Han W, 
            Haradon Z, Hastings S, Helback O, Ho WV, Huang C, Johnson T, Jones 
            DL, Juneau Z, Kenney J, Leibly M, Li S, Liang E, Loeffler H, Lusk 
            NA, Madigan Z, Malloy J, Malone J, McCue R, Melchor J, Mich JK, 
            Moosman S, Morin E, Naidoo R, Newman D, Ngo K, Nguyen K, Oster AL, 
            Ouellette B, Oyama AA, Pena N, Pham T, Phillips E, Pom C, Potekhina 
            L, Ransford S, Reding M, Rette DF, Reynoldson C, Rimorin C, Rios 
            Sigler A, Rocha DB, Ronellenfitch K, Ruiz A, Sawyer L, Sevigny J, 
            Shapovalova NV, Shepard N, Shulga L, Soliman S, Staats B, Taormina 
            MJ, Tieu M, Wang Y, Wilkes J, Wood T, Zhou T, Williford A, Dee N, 
            Mollenkopf T, Ng L, Esposito L, Kalmbach B, Yao S, Ariza J, Mufti S,
            Smith K, Waters J, Ersing I, Patrick M, Zeng H, Lein ES, Kojima Y, 
            Horwitz G, Owen SF, Levi BP, Daigle TL, Tasic B, Bakken TE, Ting JT
  TITLE     Enhancer AAV toolbox for accessing and perturbing striatal cell 
            types and circuits.
  JOURNAL   bioRxiv [Preprint]. 2024 Sep 29:2024.09.27.615553. doi: 
            10.1101/2024.09.27.615553.
  PUBMED    39386678
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Sep 29:2024.09.27.615553. doi: 
            10.1101/2024.09.27.615553."
COMMENT     Sequence Label: AiP15481:
            pAAV-AiE0140h_3xC2-minBG-iCre(R297T)...
FEATURES             Location/Qualifiers
     source          1..5123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     regulatory      837..846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             849..869
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2163..2274
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2386..2526
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2386..2515
                     /label=AAV2 ITR
     rep_origin      2601..3056
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2688..2707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2898..2919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3073..3092)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3192..3214
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3252..3270)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3338..3442
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3443..3511,3512..4303)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3661..3680)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4474..5062
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4963..4982
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtccaag aaattagaat aaagggaaaa
      181 atgactttga gatgtacttt cttaaaataa ctgaagtaat gaccgcatga gccagctgat
      241 gggtttttaa atggatacgt ttctatcagc ctgctgcttg atgccagagc caaatatata
      301 tggagtgtat tatatcatat cctgtaggca ggagactgtt ggggcattgg gggacccaag
      361 aaattagaat aaagggaaaa atgactttga gatgtacttt cttaaaataa ctgaagtaat
      421 gaccgcatga gccagctgat gggtttttaa atggatacgt ttctatcagc ctgctgcttg
      481 atgccagagc caaatatata tggagtgtat tatatcatat cctgtaggca ggagactgtt
      541 ggggcattgg gggacccaag aaattagaat aaagggaaaa atgactttga gatgtacttt
      601 cttaaaataa ctgaagtaat gaccgcatga gccagctgat gggtttttaa atggatacgt
      661 ttctatcagc ctgctgcttg atgccagagc caaatatata tggagtgtat tatatcatat
      721 cctgtaggca ggagactgtt ggggcattgg gggacgagct cgggctgggc ataaaagtca
      781 gggcagagcc atctattgct tacatttgct tctgggatcc agatctttcc aagctagcca
      841 ccatggtgcc caagaagaag aggaaagtct ccaacctgct gactgtgcac caaaacctgc
      901 ctgccctccc tgtggatgcc acctctgatg aagtcaggaa gaacctgatg gacatgttca
      961 gggacaggca ggccttctct gaacacacct ggaagatgct cctgtctgtg tgcagatcct
     1021 gggctgcctg gtgcaagctg aacaacagga aatggttccc tgctgaacct gaggatgtga
     1081 gggactacct cctgtacctg caagccagag gcctggctgt gaagaccatc caacagcacc
     1141 tgggccagct caacatgctg cacaggagat ctggcctgcc tcgcccttct gactccaatg
     1201 ctgtgtccct ggtgatgagg agaatcagaa aggagaatgt ggatgctggg gagagagcca
     1261 agcaggccct ggcctttgaa cgcactgact ttgaccaagt cagatccctg atggagaact
     1321 ctgacagatg ccaggacatc aggaacctgg ccttcctggg cattgcctac aacaccctgc
     1381 tgcgcattgc cgaaattgcc agaatcagag tgaaggacat ctcccgcacc gatggtggga
     1441 gaatgctgat ccacattggc aggaccaaga ccctggtgtc cacagctggt gtggagaagg
     1501 ccctgtccct gggggttacc aagctggtgg agagatggat ctctgtgtct ggtgtggctg
     1561 atgaccccaa caactacctg ttctgccggg tcagaaagaa tggtgtggct gccccttctg
     1621 ccacctccca actgtccacc cgggccctgg aagggatctt tgaggccacc caccgcctga
     1681 tctatggtgc caaggatgac tctgggcaga gatacctggc ctggtctggc cactctgcca
     1741 gagtgggtgc tgccaccgac atggccaggg ctggtgtgtc catccctgaa atcatgcagg
     1801 ctggtggctg gaccaatgtg aacattgtga tgaactacat cagaaacctg gactctgaga
     1861 ctggggccat ggtgaggctg ctcgaagatg gggactgagg cgcgccgcgg ccttaaagag
     1921 accggttcac tgtgacagta aaagagaccg gttcactgtg agaatgaaag agaccggttc
     1981 actgtgatcg gaaaagagac cggttcactg tgagcggcct tgaaacccag cagacaatgt
     2041 agctcagtag aaacccagca gacaatgtag ctgaatggaa acccagcaga caatgtagct
     2101 tcggagaaac ccagcagaca atgtagctgt cgacgaattc aagcttctcg agagatcttc
     2161 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     2221 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     2281 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     2341 ttgggaagac aatagcaggc atgcacgtgc ggaccgagcg gccgcaggaa cccctagtga
     2401 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     2461 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     2521 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2581 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2641 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2701 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2761 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     2821 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     2881 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     2941 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3001 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     3061 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     3121 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     3181 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3241 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3301 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3361 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3421 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3481 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3541 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3601 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     3661 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     3721 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3781 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3841 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3901 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3961 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4021 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4081 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4141 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4201 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4261 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4321 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4381 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4441 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4501 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4561 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4621 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4681 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4741 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4801 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4861 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4921 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4981 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5041 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5101 ttgctggcct tttgctcaca tgt
//
