LOCUS       sequence-454064-       10476 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Level 1, Position 3 containing 35S::RUBY::OCS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10476)
  AUTHORS   Chiang BJ, Lin KY, Chen YF, Huang CY, Goh FJ, Huang LT, Chen LH, Wu 
            CH
  TITLE     Development of a tightly regulated copper-inducible transient gene 
            expression system in Nicotiana benthamiana incorporating suicide 
            exon and Cre recombinase
  JOURNAL   bioRxiv 2024.03.23.586378
REFERENCE   2  (bases 1 to 10476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.03.23.586378"
COMMENT     Sequence Label: pCHW91003
FEATURES             Location/Qualifiers
     source          1..10476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    42..66
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(222..1370)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(1706..2294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1786..1805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2465..3256,3257..3325))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3088..3107
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3326..3430)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3517..4148
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    4240..4264
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5362..5707
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5650..5670
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5798..9748
                     /label=RUBY
     CDS             7298..7354
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8189..8245
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     terminator      9762..10469
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 ttacttgtct tctgcacgaa gtggtttaaa ctatcagtgt ttgacaggat atattggcgg
       61 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
      121 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc agatcaggcg
      181 ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc
      241 aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct
      301 tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg
      361 aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt
      421 cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca
      481 gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca
      541 cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg
      601 gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa
      661 taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc
      721 ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca
      781 tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa
      841 atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca
      901 gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac
      961 aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg
     1021 gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata
     1081 gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcaagacga
     1141 cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg
     1201 tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg
     1261 cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc
     1321 gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct
     1381 tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct
     1441 ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc
     1501 tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac cagcgacccc
     1561 ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa aacacttgat gcggaagaag
     1621 tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca tctaggatct gccaggaacc
     1681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     2161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2281 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2341 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2401 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2461 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2521 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2581 gccccagtgc tgcaatgata ccgcgagaac cacgctcacc ggctccagat ttatcagcaa
     2641 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2701 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2761 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2821 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2881 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2941 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3001 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3061 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3121 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3181 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3241 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3301 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3361 aaggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3421 gggttccgcg cacgaattgg ccagcgctgc catttttggg gtgaggccgt tcgcggccga
     3481 ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg gttcgagaag
     3541 ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa
     3601 caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa
     3661 acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc tcaaatgtca
     3721 ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat cgcgcccctc
     3781 atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc cccaggcttg
     3841 tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg
     3901 gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg
     3961 gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg gccaagtttt
     4021 ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc acacggcttc gacggcgttt
     4081 ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca accagcccgg
     4141 tgagcgtcgc aaaggagatc ctgatctgac tgatgggctg cctgtatcga gtggtgattt
     4201 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
     4261 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg
     4321 tggatgcagt gggccccact ctgtgaagac aaactagaat tcgagctcgg aggaattcca
     4381 atcccacaaa aatctgagct taacagcaca gttgctcctc tcagagcaga atcgggtatt
     4441 caacaccctc atatcaacta ctacgttgtg tataacggtc cacatgccgg tatatacgat
     4501 gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg gagttgcaca caagaaattt
     4561 gccactatta cagaggcaag agcagcagct gacgcgtaca caacaagtca gcaaacagac
     4621 aggttgaact tcatccccaa aggagaagct caactcaagc ccaagagctt tgctaaggcc
     4681 ctaacaagcc caccaaagca aaaagcccac tggctcacgc taggaaccaa aaggcccagc
     4741 agtgatccag ccccaaaaga gatctccttt gccccggaga ttacaatgga cgatttcctc
     4801 tatctttacg atctaggaag gaagttcgaa ggtgaaggtg acgacactat gttcaccact
     4861 gataatgaga aggttagcct cttcaatttc agaaagaatg ctgacccaca gatggttaga
     4921 gaggcctacg cagcaagtct catcaagacg atctacccga gtaacaatct ccaggagatc
     4981 aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ttgcatcaag
     5041 aacacagaga aagacatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa
     5101 ggcttgcttc ataaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttcct
     5161 actgaatcta aggccatgca tggagtctaa gattcaaatc gaggatctaa cagaactcgc
     5221 cgtcaagact ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat
     5281 cttcgtcaac atggtggagc acgacactct ggtctactcc aaaaatgtca aagatacagt
     5341 ctcagaagat caaagggcta ttgagacttt tcaacaaagg ataatttcgg gaaacctcct
     5401 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaaggtgg
     5461 ctcctacaaa tgccatcatt gcgataaagg aaaggctatc attcaagatc tctctgccga
     5521 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagaggttcc
     5581 aaccacgtct acaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc
     5641 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
     5701 gaggacacgc tcgagtataa gagctcattt ttacaacaat taccaacaac aacaaacaac
     5761 aaacaacatt acaattacat ttacaattat cgatacaatg gatcatgcga ccctcgccat
     5821 gatcctcgcg atctggttca tcagcttcca cttcatcaag ctgctgttct cccagcagac
     5881 caccaagctg cttccgccag gaccaaagcc gcttccgatc atcggcaaca tccttgaggt
     5941 gggcaagaag ccgcatcggt ccttcgccaa cctcgccaag attcacggcc cactcatttc
     6001 cctcagactc ggctctgtga ccaccatcgt tgtgtcctct gccgacgtgg ccaaagagat
     6061 gttcctcaag aaggatcacc cgctctccaa ccgcacgatc ccgaatagtg ttacagccgg
     6121 cgaccaccac aagctcacca tgtcttggct cccggtgtct ccgaagtggc gcaacttccg
     6181 caagattacc gccgtgcatc tgctctcccc acagagactc gatgcctgcc agacattcag
     6241 gcacgccaag gtgcagcagc tctacgagta cgttcaagag tgcgcccaga aaggccaggc
     6301 cgtggatatt ggcaaggccg cctttacgac cagcctcaac ctcctcagca agctgttctt
     6361 cagcgtcgag ctggcgcacc acaagtccca taccagccaa gagttcaaag agctgatctg
     6421 gaacatcatg gaagatatag gcaagccgaa ctacgccgac tacttcccga ttctcggctg
     6481 cgttgaccca tctggcatta gaagaaggct cgcctgctcc ttcgacaagc tgatcgccgt
     6541 gttccagggc atcatctgcg agagactcgc cccagattcc tccaccacaa ctaccaccac
     6601 caccgacgac gtgctcgatg tgctcctcca gctgttcaag cagaacgagc tgacgatggg
     6661 cgagatcaac cacctcctcg tggacatctt cgacgccggc accgatacca catcctccac
     6721 attcgagtgg gtgatgaccg agctgatccg caatccagag atgatggaaa aggcccaaga
     6781 ggaaatcaag caggtcctcg gcaaggacaa gcagatccaa gagtccgaca tcatcaacct
     6841 gccgtacctc caggcgatca tcaaagagac actccgcctc catccgccga ccgtgttctt
     6901 gctcccaaga aaggccgaca ccgatgtcga gctgtacggc tacatcgtgc cgaaggatgc
     6961 ccagatcctc gtgaacctct gggccattgg cagggaccca aacgcctggc agaacgccga
     7021 tattttcagc ccagagcgct tcatcggctg cgagatcgat gttaagggcc gcgatttcgg
     7081 cctccttcca tttggcgctg gccgcagaat ttgcccaggc atgaatctcg ccatcaggat
     7141 gctcaccctc atgctcgcca cactcctcca gttcttcaac tggaagctcg aaggcgacat
     7201 ctccccgaag gacctcgaca tggacgagaa gttcggcatt gcgctccaaa agaccaagcc
     7261 gctcaagctc atcccgattc cgcgctacgg tagcggagct accaatttta gcctccttaa
     7321 gcaggcaggt gatgtagaag agaaccccgg gcctatgaag atgatgaacg gcgaggacgc
     7381 caacgaccag atgatcaaag agtccttctt catcacccac ggcaacccga tcctcaccgt
     7441 cgaggataca catccgctca ggccgttctt cgagacatgg cgcgagaaga ttttctccaa
     7501 gaagccgaag gccatcctca tcatctccgg ccactgggag acagtgaagc caaccgtgaa
     7561 cgccgtgcac atcaacgaca ccatccacga cttcgacgac tacccagccg ccatgtacca
     7621 gttcaagtac ccagctccag gcgagccaga gcttgcgaga aaggtggaag agatcctcaa
     7681 gaagtccggg ttcgagacag ccgagacaga ccaaaagagg ggccttgatc acggcgcctg
     7741 ggttccactc atgctcatgt atccagaggc ggacatcccg gtgtgccagc tctcagttca
     7801 gccacatctc gacggcacct accactacaa tctcggcaga gccctcgcgc cgctcaagaa
     7861 tgatggcgtg ctcattattg gctccggcag cgccacacat ccactcgatg agacaccgca
     7921 ctacttcgat ggtgttgccc cttgggccgc tgccttcgat tcttggctta ggaaggccct
     7981 catcaacggc cgcttcgagg aagtgaacat ctacgagagc aaggccccga actggaagct
     8041 cgcccatcca tttccagagc acttctaccc gctccacgtt gtgctcggcg ctgctggtga
     8101 aaagtggaag gccgagctga tccactcctc ctgggatcat ggcacacttt gccacggctc
     8161 ctacaagttc acctccgccg gatccggagc aaccaacttt agcctgctca agcaagcagg
     8221 agatgttgag gaaaatcctg gccccatgac cgccatcaag atgaacacca acggcgaggg
     8281 cgagacacag cacatcctca tgatcccgtt catggcgcag ggccacctca ggccatttct
     8341 cgaactcgcc atgttcctct acaagcgctc ccacgtgatc atcaccctgc tcacaactcc
     8401 gctcaatgcc ggcttcctca ggcacctcct tcaccaccat tcctactcct ccagcggcat
     8461 caggatcgtc gagctgccat tcaactccac caaccacgga ctcccaccgg gcatcgagaa
     8521 caccgataag ctcacactcc cgctcgtggt gtccctcttc cattccacca tcagcctcga
     8581 tccgcacctc cgcgattaca tctccaggca tttcagccca gccaggccac cactctgcgt
     8641 gatccatgat gtgttcctcg gctgggttga ccaggtggcc aaggatgtgg gctctacagg
     8701 cgtggtgttc acaacaggcg gcgcttatgg cacatccgcc tacgtgtcca tctggaacga
     8761 tctcccgcac cagaactact ccgacgacca agagttcccg ctgccaggct tcccagagaa
     8821 ccataagttc cgcaggtccc agctccatcg gttcctcaga tatgccgacg gctccgacga
     8881 ttggtccaag tatttccagc cgcagctccg ccagtccatg aagtcttttg gctggctctg
     8941 caactccgtg gaagagatcg agacactcgg cttctccatc ctccgcaact acaccaagct
     9001 gccgatctgg ggcatcggcc cacttattgc ttccccagtg cagcactcct cctccgacaa
     9061 caattcaaca ggcgccgagt tcgtgcagtg gctcagcctc aaagagccgg actccgtcct
     9121 ctacatctcc ttcggctccc agaacacgat cagcccgacg cagatgatgg aactcgctgc
     9181 tggccttgag tcctccgaga agccattcct ctgggtgatc agagccccgt tcggcttcga
     9241 catcaacgaa gagatgcgcc cagagtggct gccagagggc tttgaggaac gcatgaaggt
     9301 gaagaaacag ggcaagctcg tgtacaagct cggcccgcag cttgagatcc tcaaccatga
     9361 atccatcggc ggctttctca cccactgcgg atggaacagc atccttgagt ctcttcgcga
     9421 gggcgttccg atgcttggat ggccacttgc tgccgagcag gcctacaacc tcaagtacct
     9481 cgaagatgag atgggcgtcg cggttgagct tgctagaggc ctcgaaggcg agatctccaa
     9541 agagaaggtc aagcgcatcg tcgagatgat ccttgagcgc aacgagggct ccaaaggctg
     9601 ggagatgaag aatcgcgccg tggaaatggg caaaaagctc aaggacgccg tgaacgagga
     9661 aaaagagctg aagggctcct ccgtgaaggc gatcgacgat ttcctcgacg ccgtcatgca
     9721 ggccaaactt gagccaagcc tccagtgata gtgagcttgt cctgctttaa tgagatatgc
     9781 gagaagccta tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc
     9841 tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt cattctaatg aatatatcac
     9901 ccgttactat cgtattttta tgaataatat tctccgttca atttactgat tgtaccctac
     9961 tacttatatg tacaatatta aaatgaaaac aatatattgt gctgaatagg tttatagcga
    10021 catctatgat agagcgccac aataacaaac aattgcgttt tattattaca aatccaattt
    10081 taaaaaaagc ggcagaaccg gtcaaaccta aaagactgat tacataaatc ttattcaaat
    10141 ttcaaaagtg ccccaggggc tagtatctac gacacaccga gcggcgaact aataacgctc
    10201 actgaaggga actccggttc cccgccggcg cgcatgggtg agattccttg aagttgagta
    10261 ttggccgtcc gctctaccga aagttacggg caccattcaa cccggtccag cacggcggcc
    10321 gggtaaccga cttgctgccc cgagaattat gcagcatttt tttggtgtat gtgggcccca
    10381 aatgaagtgc aggtcaaacc ttgacagtga cgacaaatcg ttgggcgggt ccagggcgaa
    10441 ttttgcgaca acatgtcgag gctcagcagg accgct
//
