LOCUS       sequence-454093-        3102 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Level 0, CDS module (AATG-GCTT) containing Cre recombinase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Chiang BJ, Lin KY, Chen YF, Huang CY, Goh FJ, Huang LT, Chen LH, Wu 
            CH
  TITLE     Development of a tightly regulated copper-inducible transient gene 
            expression system in Nicotiana benthamiana incorporating suicide 
            exon and Cre recombinase
  JOURNAL   bioRxiv 2024.03.23.586378
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.03.23.586378"
COMMENT     Sequence Label: pCHW110033
FEATURES             Location/Qualifiers
     source          1..3102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      640..1228
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1129..1148
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2610..3102,1..299)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
       61 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
      121 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
      181 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
      241 agttggaaga atttgtccat tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
      301 gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt
      361 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
      421 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc tgtcagacca
      481 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
      541 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
      601 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
      661 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
      721 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
      781 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
      841 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
      901 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      961 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1021 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1081 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1141 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1201 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1261 gcactctgtg gtctcaaatg tccaacctgc tcacggttca ccagaacctt ccggctcttc
     1321 cagtggacgc gacgtccgat gaagtcagga agaacctcat ggacatgttc cgcgacaggc
     1381 aagcgttcag cgagcacacc tggaagatgc tgctctccgt ctgccgctcc tgggctgcat
     1441 ggtgcaagct gaacaacagg aagtggttcc ccgctgagcc cgaggacgtg agggattacc
     1501 ttctgtacct gcaagcgcga ggtctggcag tgaaaaccat ccagcaacac cttggacaac
     1561 tgaacatgct tcacaggcgc tccggcctcc cgcgccccag cgactcgaac gccgtgagcc
     1621 tcgtcatgcg ccgcatcagg aaggaaaacg tcgatgccgg cgaaagggca aagcaggccc
     1681 tcgcgttcga gagtaccgat ttcgaccagg tccgcagcct gatggagaac agcgacaggt
     1741 gccaggacat taggaacctg gcgttcctcg gaattgcata caacacgctc ctcaggatcg
     1801 cggaaattgc ccgcattcgc gtgaaggaca ttagccgcac cgacggcggc aggatgctta
     1861 tccacattgg caggaccaag acgctcgttt ccaccgcagg cgtcgaaaag gccctcagcc
     1921 tcggagtgac caagctcgtc gaacgctgga tctccgtgtc cggcgtcgcg gacgacccaa
     1981 acaactacct cttctgccgc gtccgcaaga acggggtggc tgcccctagc gccaccagcc
     2041 aactcagcac gagggccttg gaaggtattt tcgaggccac ccaccgcctg atctacggcg
     2101 cgaaggatga cagcggtcaa cgctacctcg catggtccgg gcactccgcc cgcgttggag
     2161 ctgctaggga catggcccgc gccggtgttt ccatccccga aatcatgcag gcgggtggat
     2221 ggacgaacgt gaacattgtc atgaactaca ttcgcaacct tgacagcgag acgggcgcaa
     2281 tggttcgcct cctggaagat ggtgactgag cttcgagacc acgaagtgcc gaggcacgaa
     2341 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     2401 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     2461 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     2521 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     2581 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     2641 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     2701 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat attgatttgc
     2761 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     2821 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     2881 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     2941 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     3001 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     3061 aggaactctt tgatccggtt cctgaacagg atctatttga gg
//
