LOCUS       sequence-454112-        3348 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express mEGFP-fusion protein of FMR1 fragment (1-491) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Chen X, Fansler MM, Janjos U, Ule J, Mayr C
  TITLE     The FXR1 network acts as signaling scaffold for actomyosin 
            remodeling.
  JOURNAL   Cell. 5 August 2024. doi: 10.1016/j.cell.2024.07.015.
  PUBMED    37961296
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 5 
            August 2024. doi: 10.1016/j.cell.2024.07.015."
COMMENT     Sequence Label: pcDNA3puro-mGFP-FMR1iso1-N2(1-491)
FEATURES             Location/Qualifiers
     source          1..3348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(248..266)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        334..438
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(439..507,508..1299)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(657..676)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1470..2058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1959..1978
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2212..2229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2469..2490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2505..2510,2511..2528,2529..2535)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2543..2559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2548..2570
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2567..2583
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2567..2583
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2633..2652
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(2844..3348,1..129)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
       61 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      121 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
      181 gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga
      241 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      301 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      361 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      421 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      481 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      541 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
      601 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
      661 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
      721 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
      781 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
      841 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      901 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
      961 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     1021 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     1081 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1141 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     1201 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1261 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1321 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1381 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1441 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1501 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1561 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1621 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1681 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1741 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1801 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1861 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1921 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1981 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2041 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2101 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2161 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2221 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     2281 attcattaat gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg
     2341 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     2401 tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     2461 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     2521 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     2581 gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     2641 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     2701 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     2761 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     2821 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     2881 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     2941 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     3001 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     3061 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     3121 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     3181 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     3241 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     3301 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactag
//
