LOCUS       sequence-454146-        4172 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway clone for S3x6-JunB promoter TGF-beta pathway reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4172)
  TITLE     Esposito Lab Plasmids 2024
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 18065-E01
FEATURES             Location/Qualifiers
     source          1..4172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..179
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1604..1728
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2139..2930
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3023..3611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3512..3531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3765..3782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3941..3968)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4060..4146)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccaaataat gattttattt tgactgatag tgacctgttc
      121 gttgcaacaa attgataagc aatgcttttt tataatgcca actttgtata gaaaagttgg
      181 cgtttatctt cagctggagt cagggaaaca gcgtctcggg aacatcctgt ggactgctag
      241 ctggacagac aagagggtca gtccctccca gggcagggca gacataggct gggggcaaag
      301 taaacatgcc ccagggtggc tagactgcgg gagatctggt cgttcagtca ctccaccagt
      361 agctgatcag tagataactt gaggtgaggc tcagatctgg aaacagcgtc tcgggaacat
      421 cctgtggact gctagctgga cagacaagag ggtcagtccc tcccagggca gggcagacat
      481 aggctggggg caaagtaaac atgccccagg gtggctagac tgcggggaat tccactacag
      541 gccgggtatt taaaaccgcg ggcccaataa gcggcatcat atgaaatgaa ttcggaaaca
      601 gcgtctcggg aacatcctgt ggactgctag ctggacagac aagagggtca gtccctccca
      661 gggcagggca gacataggct gggggcaaag taaacatgcc ccagggtggc tagactgcgg
      721 gccatgggcc gcgcggaccc tcccatgacg tatgcatcgt atgctagtgc tgttatggag
      781 tgccatgggg aaacagcgtc tcgggaacat cctgtggact gctagctgga cagacaagag
      841 ggtcagtccc tcccagggca gggcagacat aggctggggg caaagtaaac atgccccagg
      901 gtggctagac tgcgggtcgc gaattgtgcg cactttaccg gcggggacac catgaagtgt
      961 gcttctgctg accatcctcg cgaggaaaca gcgtctcggg aacatcctgt ggactgctag
     1021 ctggacagac aagagggtca gtccctccca gggcagggca gacataggct gggggcaaag
     1081 taaacatgcc ccagggtggc tagactgcgg ggtttaaaca ctgcccgccg aaaaatggat
     1141 aagcatagta agctgcataa gtggacaaca ctcagtttaa acggaaacag cgtctcggga
     1201 acatcctgtg gactgctagc tggacagaca agagggtcag tccctcccag ggcagggcag
     1261 acataggctg ggggcaaagt aaacatgccc cagggtggct agactgcggg tcgaggaagc
     1321 ccctccccct gcagccgcgc agagccaccc ggctcgtggc cgctgtttac aaggacacgc
     1381 gcttcctgac agtgacgcga gccgcctcct ccccttcccc acgctctagg agggggccgc
     1441 gggggcctgg ctcccgcgtc ggccaatcgg agtgcacttc cgcagctgac aaattcagta
     1501 taaaagcttg gggctggggc cgagcactgg ggactttgag ggtggccagg ccagcgtagg
     1561 atcctgctgg gagcggggaa ctgagggaag cgacgccgat aaaacaactt tgtacaaaaa
     1621 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1681 aaaacagact acataatact gtaaaacaca acatatgcag tcactatgaa tcaactactt
     1741 agatggtatt agtgacctgt agcatgcaag cttagatcct ctagaccagc caggacagaa
     1801 atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
     1861 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
     1921 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     1981 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     2041 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     2101 cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg
     2161 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     2221 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     2281 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     2341 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     2401 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     2461 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     2521 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     2581 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     2641 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     2701 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     2761 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     2821 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     2881 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc
     2941 acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     3001 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3061 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3781 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     3841 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     3901 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     3961 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     4021 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     4081 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     4141 tatttgatgc ctggcagttc cctactctcg cg
//
