LOCUS       sequence-454867-        3024 bp    DNA     circular SYN 15-APR-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3024)
  TITLE     Biosynthesis of anti-inflammatory triterpene fatty acid esters in 
            Calendula officinalis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEPCT0CM0209
FEATURES             Location/Qualifiers
     source          1..3024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1068..1084)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1068..1084)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1081..1103)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1256..1844)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1336..1355)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1977..2071)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2092..2751)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2666..2685
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2752..2854)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2941..2963
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2955..2972
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2956..2972
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ataggtctct aatggtgtgg ggatgtttat taattgcaca aggtacaact agattcttgg
       61 cactatttcc agtcatatgt ctctttgtat gccttcccat taatcttacc accatatttc
      121 ttggtggtct tacttctttc ttcatttcat ggcttggaag tttcaagctc atcctttatg
      181 cctttggcca aggtccttta tcttcatatc cacatctccc tatgtatcac ttcatattta
      241 ctgcttgctt acccatcaaa atcgttaaaa ttcaagaaaa accatcttac cacattacca
      301 agaaatcccc catacattat gctcctcggg ctatcctgtt tatcgtcgca attaaggcgc
      361 atagttacaa agattatttt cacccgttat ttaccacatc tcttatttca ttttatatat
      421 tttgcatgct cgaattgatg ctagccgtga tagcttatct agctcaaaca atagttgggt
      481 ttgagctaga gccacaattt gatgagccac accatgccac atccgtacaa aacttttggg
      541 ggaaaaggtg gaacctcatg gcgtctagca tactccgccc cacggtctat catccttccc
      601 gagccgtctt tagccatctg gttcctaaaa gatgggtatc ggtgccagct gtccttacaa
      661 cgtttctagt atccgggata gtgcacgagt tgatctttta ttaccttggg cggttcacgc
      721 caacatggga aatcacttgg ttctttgtag tccaaggtgc ttgggttgga atcgagattg
      781 tggtcaagaa aaccacgggt caacggctcg agccaccaac cgttgtggcc cggactttaa
      841 ctttggcgtt tgtgatgctt actagctttt ggcttttctt cccaccattc atgcaactag
      901 atgccctttt gagagcttgt aaagaggcca cggcgtttgt tggatttcta aagagtggtc
      961 atctagttag cccaagtcag tatgcatgtc cactcatgtt cgagagacct aaggctaggt
     1021 ggaggctcag tgatgataag tctgcgatgg tggatgcatg tgtcatggtc atagctgttt
     1081 cctgtgtgaa attgttatcc gctcagaggg cacaatccta ttccgcgcta tccgacaatc
     1141 tccaagacat taggtggagt tcagttcggc gtatggcata tgtcgctgga aagaacatgt
     1201 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1261 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1321 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1381 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1441 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1501 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1561 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1621 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1681 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1741 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1801 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1861 tttctacggg gtctgacgct ctattcaaca aagccgccgt cccgtcaagt cagcgtaaat
     1921 gggtaggggg cttcaaatcg tccccccata cgatataagt tgttactagt gcttggattc
     1981 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     2041 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
     2101 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     2161 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     2221 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     2281 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
     2341 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     2401 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     2461 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     2521 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     2581 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     2641 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     2701 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     2761 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     2821 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattgatacc tgaaacaaaa
     2881 cccatcgtac ggccaaggaa gtctccaata actgtgatcc accacaagcg ccagggtttt
     2941 cccagtcacg acgttgtaaa acgacggcca gtcatgcata atccgcacgc atctggaata
     3001 aggaagtgcc attccgcctg acct
//
