LOCUS       sequence-454976-       10792 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2536
FEATURES             Location/Qualifiers
     source          1..10792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6649..7237
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6702..6722)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7120..7131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7309..7542
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7620..7741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7657..7676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7711..7730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7781..7916
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7936..7955)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7937..7955)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7965..7982)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7965..7981)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7974..7996)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8123..8578
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8210..8229)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8420..8441
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8604..8708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8709..8777,8778..9569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8927..8946)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9740..10328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10229..10248
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10482..10499
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10616..10637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10652..10657,10658..10675,10676..10682)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10690..10706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10695..10717
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10714..10730
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10714..10730
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10749..10769
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10751..10769
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtct tccaagcaag ccacctctcc
     4141 atttgcctgt gcagctgatg gagaggatgc aatgacccag gatttaacct caagggaaaa
     4201 ggaagagggc agtgatcaac atgtggcctc ccatctgcct ctgcacccca taatgcacaa
     4261 caaacctcac tctgaggagc taccaacact tgtcagtacc attcaacaag atgctgactg
     4321 ggacagcgtt ctgtcatctc agcaaagaat ggaatcagag aataataagt tatgttccct
     4381 atattccttc cgaaatacct ctacctcacc acataagcct gacgaaggga gtcgggaccg
     4441 tgagataatg accagtgtta cttttggaac cccagagcgc cgcaaaggga gtcttgccga
     4501 tgtggtggac acactgaaac agaagaagct tgaggaaatg actcggactg aacaagagga
     4561 ttcctcctgc atggaaaaac tactttcaaa agattggaag gaaaaaatgg aaagactaaa
     4621 taccagtgaa cttcttggag aaattaaagg tacacctgag agcctggcag aaaaagaacg
     4681 gcagctctcc accatgatta cccagctgat cagtttacgg gagcagctac tggcagcgca
     4741 tgatgaacag aaaaaactgg cagcgtcaca aattgagaaa caacggcagc aaatggacct
     4801 tgctcgccaa cagcaagaac agattgcgag acaacagcag caacttctgc aacagcagca
     4861 caaaattaat ctcctgcagc aacagatcca ggttcagggt cacatgcctc cgctcatgat
     4921 cccaattttt ccacatgacc agcggactct ggcagcagct gctgctgccc aacagggatt
     4981 cctcttcccc cctggaataa catacaaacc aggtgataac taccccgtac agttcattcc
     5041 atcaacaatg gcagctgctg ctgcttctgg actcagccct ttacagctcc agaagggtca
     5101 tgtctcccac ccacaaatta accaaaggct aaagggccta agtgaccgtt ttggcaggaa
     5161 tttggacacc tttgaacatg gtggtggcca ctcttacaac cacaaacaga ttgagcagct
     5221 ctatgccgct cagctggcca gcatgcaggt gtcacctgga gcaaagatgc catcaactcc
     5281 acagccacca aacacagcag ggacggtctc acctactggg ataaaaaatg aaaagagagg
     5341 gaccagccct gtaactcaag ttaaggatga agcagcagca cagcctctga atctctcatc
     5401 ccgacccaag acagcagagc ctgtaaagtc cccaacgtct cccacccaga acctcttccc
     5461 agccagcaaa accagccctg tcaatctgcc aaacaaaagc agcatcccta gccccattgg
     5521 aggaagcctg ggaagaggat cctctttaga tatcctatct agtctcaact cccctgccct
     5581 ttttggggat caggatacag tgatgaaagc cattcaggag gcgcggaaga tgcgagagca
     5641 gatccagcgg gagcaacagc agcaacagcc acatggtgtt gacgggaaac tgtcctccat
     5701 aaataatatg gggctgaaca gctgcaggaa tgaaaaggaa agaacgcgct ttgagaattt
     5761 ggggccccag ttaacgggaa agtcaaatga agatggaaaa ctgggcccag gtgtcatcga
     5821 ccttactcgg ccagaagatg cagagggagg tgccactgtg gctgaagcac gagtctacag
     5881 ggacgcccgc ggccgtgcca gcagcgagcc acacattaag cgaccaatga atgcattcat
     5941 ggtttgggca aaggatgaga ggagaaaaat ccttcaggcc ttccccgaca tgcataactc
     6001 caacattagc aaaatcttag gatctcgctg gaaatcaatg tccaaccagg agaagcaacc
     6061 ttattatgaa gagcaggccc ggctaagcaa gatccactta gagaagtacc caaactataa
     6121 atacaaaccc cgaccgaaac gcacctgcat tgttgatggc aaaaagcttc ggattgggga
     6181 gtataagcaa ctgatgaggt ctcggagaca ggagatgagg cagttcttta ctgtggggca
     6241 acagcctcag attccaatca ccacaggaac aggtgttgtg tatcctggtg ctatcactat
     6301 ggcaactacc acaccatcgc ctcagatgac atctgactgc tctagcacct cggccagccc
     6361 ggagcccagc ctcccggtca tccagagcac ttatggtatg aagacagatg gcggaagcct
     6421 agctggaaat gaaatgatca atggagagga tgaaatggaa atgtatgatg actatgaaga
     6481 tgaccccaaa tcagactata gcagtgaaaa tgaagccccg gaggctgtca gtgccaacta
     6541 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     6601 aaaggacgaa cctggtgatc cagtggcata gttacgcgtt aagtcgacaa tcaacctctg
     6661 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6721 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6781 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6841 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6901 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6961 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7021 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7081 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7141 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7201 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     7261 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7321 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7381 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7441 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7501 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     7561 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     7621 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7681 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7741 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     7801 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7861 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7921 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     7981 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8041 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     8101 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     8161 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     8221 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     8281 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     8341 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     8401 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     8461 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     8521 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     8581 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8641 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8701 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8761 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8821 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8881 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8941 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9001 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9061 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9121 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9181 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9241 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9301 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9361 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9421 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9481 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9541 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9601 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9661 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9721 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9781 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9841 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     9901 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9961 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10021 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10081 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10141 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10201 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10261 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10321 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10381 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10441 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10501 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    10561 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    10621 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    10681 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    10741 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gc
//
