LOCUS       sequence-454979-       11692 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11692)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0089
FEATURES             Location/Qualifiers
     source          1..11692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7549..8137
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7602..7622)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8020..8031)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8209..8442
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8520..8641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8557..8576)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8611..8630
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8681..8816
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8836..8855)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8837..8855)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8865..8882)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8865..8881)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8874..8896)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9023..9478
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9110..9129)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9320..9341
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9504..9608
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9609..9677,9678..10469)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9827..9846)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10640..11228
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11129..11148
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11382..11399
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11516..11537
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11552..11557,11558..11575,11576..11582)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11590..11606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11595..11617
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11614..11630
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11614..11630
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11649..11669
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11651..11669
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaaa gttacaaatt ataatactgt
     4141 ggtagaaaca aattcagatt cagatgatga agacaaactg catattgtgg aagaagaaag
     4201 tgttacagat gcagctgact gtgaaggtgt accagaggat gacctgccaa cagaccagac
     4261 agtgttacca gggaggagca gtgaaagaga agggaatgct aagaactgct gggaggatga
     4321 cacaggaaag gaagggcaag aaatcctggg gcctgaagct caggcagatg aagcaggatg
     4381 tacagtaaaa gatgatgaat gcgagtcaga tgcagaaaat gagcaaaacc atgatcctaa
     4441 tgttgaagag tttctacaac aacaagacac tgctgtcatt tttcctgagg cacctgaaga
     4501 ggaccagagg cagggcacac cagaagccag tggtcatgat gaaaatggaa caccagatgc
     4561 attttcacaa ttactcacct gtccatattg tgatagaggc tataaacgct ttacctctct
     4621 gaaagaacac attaaatatc gtcatgaaaa gaatgaagat aactttagtt gctccctgtg
     4681 cagttacacc tttgcataca gaacccaact tgaacgtcac atgacatcac ataaatcagg
     4741 aagagatcaa agacatgtga cgcagtctgg gtgtaatcgt aaattcaaat gcactgagtg
     4801 tggaaaagct ttcaaataca aacatcacct aaaagagcac ttaagaattc acagtggaga
     4861 gaagccatat gaatgcccaa actgcaagaa acgcttttcc cattctggct cctatagctc
     4921 acacataagc agtaagaaat gtatcagctt gatacctgtg aatgggcgac caagaacagg
     4981 actcaagaca tctcagtgtt cttcaccgtc tctttcagca tcaccaggca gtcccacacg
     5041 accacagata cggcaaaaga tagagaataa accccttcaa gaacaacttt ctgttaacca
     5101 aattaaaact gaacctgtgg attatgaatt caaacccata gtggttgctt caggaatcaa
     5161 ctgttcaacc cctttacaaa atggggtttt cactggtggt ggcccattac aggcaaccag
     5221 ttctcctcag ggcatggtgc aagctgttgt tctgccaaca gttggtttgg tgtctcccat
     5281 aagtatcaat ttaagtgata ttcagaatgt acttaaagtg gcggtagatg gtaatgtaat
     5341 aaggcaagtg ttggagaata atcaagccaa tcttgcatcc aaagaacaag aaacaatcaa
     5401 tgcttcaccc atacaacaag gtggccattc tgttatttca gccatcagtc ttcctttggt
     5461 tgatcaagat ggaacaacca aaattatcat caactacagt cttgagcagc ctagccaact
     5521 tcaagttgtt cctcaaaatt taaaaaaaga aaatccagtc gctacaaaca gttgtaaaag
     5581 tgaaaagtta ccagaagatc ttactgttaa gtctgagaag gacaaaagct ttgaaggggg
     5641 ggtgaatgat agcacttgtc ttctgtgtga tgattgtcca ggagatatta atgcacttcc
     5701 agaattaaag cactatgacc taaagcagcc tactcagcct cctccactcc ctgcagcaga
     5761 agctgagaag cctgagtcct ctgtttcatc agctactgga gatggcaatt tgtctcctag
     5821 tcagccacct ttaaagaacc tcttgtctct cctaaaagca tattatgctt tgaatgcaca
     5881 accaagtgca gaagagctct caaaaattgc tgattcagta aacctaccac tggatgtagt
     5941 aaaaaagtgg tttgaaaaga tgcaagctgg acagatttca gtgcagtctt ctgaaccatc
     6001 ttctcctgaa ccaggcaaag taaatatccc tgccaagaac aatgatcagc ctcaatctgc
     6061 aaatgcaaat gaaccccagg acagcacagt aaatctacaa agtcctttga agatgactaa
     6121 ctccccagtt ttaccagtgg gatcaaccac caatggttcc agaagtagta caccatcccc
     6181 atcacctcta aacctttcct catccagaaa tacacagggt tacttgtaca cagctgaggg
     6241 tgcacaagaa gagccacaag tagaacctct tgatctttca ctaccaaagc aacagggaga
     6301 attattagaa aggtcaacta tcactagtgt ttaccagaac agtgtttatt ctgtccagga
     6361 agaacccttg aacttgtctt gcgcaaaaaa ggagccacaa aaggacagtt gtgttacaga
     6421 ctcagaacca gttgtaaatg taatcccacc aagtgccaac cccataaata tcgctatacc
     6481 tacagtcact gcccagttac ccacaatcgt ggccattgct gaccagaaca gtgttccatg
     6541 cttaagagcg ctagctgcca ataagcaaac gattctgatt ccccaggtgg catacaccta
     6601 ctcaactacg gtcagccctg cagtccaaga accacccttg aaagtgatcc agccaaatgg
     6661 aaatcaggat gaaagacaag atactagctc agaaggagta tcaaatgtag aggatcagaa
     6721 tgactctgat tctacaccgc ccaaaaagaa aatgcggaag acagaaaatg gaatgtatgc
     6781 ttgtgatttg tgtgacaaga tattccaaaa gagtagttca ttattgagac ataaatatga
     6841 acacacaggt aaaagacctc atgagtgtgg aatctgtaaa aaggcattta aacacaaaca
     6901 tcatttgatt gaacacatgc gattacattc tggagaaaag ccctatcaat gtgacaaatg
     6961 tggaaagcgc ttctcacact ctgggtctta ttctcaacac atgaatcatc gctactccta
     7021 ctgtaagaga gaagcggaag aacgtgacag cacagagcag gaagaggcag ggcctgaaat
     7081 cctctcgaat gagcacgtgg gtgccagggc gtctccctca cagggcgact cggacgagag
     7141 agagagtttg acaagggaag aggatgaaga cagtgaaaaa gaggaagagg aggaggataa
     7201 agagatggaa gaattgcagg aagaaaaaga atgtgaaaaa ccacaagggg atgaggaaga
     7261 ggaggaggag gaggaagaag tggaagaaga agaggtagaa gaggcagaga atgagggaga
     7321 agaagcaaaa actgaaggtc tgatgaagga tgacagggct gaaagtcaag caagcagctt
     7381 aggacaaaaa gtaggcgaga gtagtgagca agtgtctgaa gaaaagacaa atgaagccta
     7441 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     7501 aaaggacgac gtgtcgagca gcttcgaggc aacacgcgtt aagtcgacaa tcaacctctg
     7561 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7621 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7681 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7741 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7801 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7861 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7921 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7981 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8041 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8101 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     8161 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8221 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8281 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8341 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8401 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     8461 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     8521 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8581 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8641 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     8701 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8761 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8821 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     8881 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8941 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     9001 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     9061 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     9121 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9181 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     9241 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9301 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9361 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9421 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     9481 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9541 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9601 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9661 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9721 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9781 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9841 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9901 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9961 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10021 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10081 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10141 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10201 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10261 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10321 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10381 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10441 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10501 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10561 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10621 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10681 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10741 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10801 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10861 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10921 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10981 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11041 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11101 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11161 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11221 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11281 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11341 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11401 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11461 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11521 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11581 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    11641 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gc
//
