LOCUS       sequence-455186-        4339 bp    DNA     circular SYN 15-APR-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4339)
  TITLE     Biosynthesis of anti-inflammatory triterpene fatty acid esters in 
            Calendula officinalis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEPMS0CM0033
FEATURES             Location/Qualifiers
     source          1..4339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2383..2399)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2383..2399)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2396..2418)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2571..3159)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2651..2670)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3292..3386)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3407..4066)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3981..4000
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4067..4169)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4256..4278
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4270..4287
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4271..4287
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taggtctcta atgtggaagc tgaaaattgg tgcgggaaat gacgacgaat atttgtacag
       61 cacaaacaat tttgtgggac gtcaaacgtg ggagttcgat cccgatgcag gcacgcagga
      121 agaacgtgac caagtcgaac gagctagaga gcagttcttg atcaataaga aaaagcttga
      181 cataagttgt tgcggggatt tgctaatgag agctcagctt attaaggaaa gcgggattga
      241 tctactgagc gaacctccaa taagactcgg agaagaagag gacgtgagct atgaagcggt
      301 gacgacatcg gttaaaaaag cagtacgatt gaaccgtgcg attcaagctt gggacggtca
      361 ttggccagcc gaaaatgcgg gtcccctctt cttcacccca cctctgataa ttgctctcta
      421 cataagtggc acattggact caattctaac caatgaacac aagaaggaaa tgattcgcta
      481 catgcacatc catcaaaatg aggacggagg atggggattc tatatatcag gaagaagcac
      541 aatgataggg agtgctctga actacgtagc tctaaggctt ctcggagaag ctcctccgcc
      601 agatggcgac gatggtccac ttggtagagg ccgcaaatgg atacatgatc acggtggcgc
      661 cacctccatc ccatcatggg gaaaggttta tctcgcggtg cttggagtat atgaatggga
      721 aggctgcaac cctcttcctc cagaattctg gctttttcct tcctttttgc cttatcatcc
      781 agcaaaaatg tggtgttact gtcgaaccac atacatgcca atgtcacatt tgtatggcaa
      841 gagctaccat ggtcccatta cagatcttgt tttgtcttta agaaaagaga ttcatcccat
      901 cccttaccac cacattaatt ggaacaaaca acgacataac tgttgcaagg aggatctgta
      961 ctaccctcat tcattgatac aagatctact atgggacgga cttcatcatt ttagcgaacc
     1021 tatcatcaga aaatggccat tcaataagct aagagaaagg ggtcttaaaa gagttgttga
     1081 cctaatgcgc tatggagccg aggaaggacg atatatagcc atgggctgcg ttgataaggc
     1141 tttacaaata atgagttttt atgcggagga tccaaatggg atcgacttca aacgacatct
     1201 tgctagagtc cctgattact tgtgggtggc agaggatggt atgaagatgc aaagttttgg
     1261 cagtcaatta tgggattgca ctcttgtaac acaagcaatt atcgcaagta atatggtaga
     1321 cgagtatgga gattcactca aaaaagctca cttttacttg aaacaatcac agataaaaga
     1381 gaaccctaaa ggcgatttca ccaaaatgtg tcgccaattc acgaaagggg catggacttt
     1441 ctctgatcaa gatcacggtt gggtcgtgtc agattgcact gccgaagctt tgaagtgttt
     1501 attagcacta tcacaaatgc ctcaagacat tgttggagaa aaggctcaag ttgatcgatt
     1561 atatgatgct atcaatgtcc tcctttacct acaaagtcct gcaaccggtg gttttgcaat
     1621 ttgggagcct ccggttccaa aaccatattt agagaaattg aatccttcag aactttttgc
     1681 agatattgtt gtcgagaaag agcatgttga atgtaccggg tctgtaattc aagcgttgca
     1741 tgcattcaaa catctacatc ctgggtaccg tgagaaagaa atagaagttg ccattgagaa
     1801 aggcatacag tttttggaaa acaaacaaaa ggatgatggg tcgtggtatg gttactgggg
     1861 catatgtttt ctctatggca cattcttcgt gctacaagga ttggtatcgt gtgggaaaac
     1921 atatgaaaat agtgaagcaa ttcggagagc tgttcagttt ttgctcttga cacggaactt
     1981 agaaggtggt tggggagaga gcttcgagtc atgcccaaaa gagaaattca tacctttgga
     2041 ggggaatcga acaaatttgg tgcaaacttc atgggccatg ctcggacttc tctactgtgg
     2101 acaggctgaa agggatccaa caccattaca caaggcagca aaactgctca tcaatggaca
     2161 attggaaaat ggagattttc ttcaacagga gataacggga tcgtacatga aaaactgcat
     2221 gctacattat ccggagtata ggaacacttt tccgttatgg gcactcggag aataccgaaa
     2281 gcgtgtttgg ttgccgaaac aagaagccat cggttcgaga gacctaaggc taggtggagg
     2341 ctcagtgatg ataagtctgc gatggtggat gcatgtgtca tggtcatagc tgtttcctgt
     2401 gtgaaattgt tatccgctca gagggcacaa tcctattccg cgctatccga caatctccaa
     2461 gacattaggt ggagttcagt tcggcgtatg gcatatgtcg ctggaaagaa catgtgagca
     2521 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2581 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2641 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2701 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2761 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2821 tgtgtgcacg aaccccccgt tcagctcgac cgctgcgcct tatccggtaa ctatcgtctt
     2881 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2941 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3001 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3061 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3121 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3181 acggggtctg acgctctatt caacaaagcc gccgtcccgt caagtcagcg taaatgggta
     3241 gggggcttca aatcgtcccc ccatacgata taagttgtta ctagtgcttg gattctcacc
     3301 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     3361 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     3421 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     3481 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     3541 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     3601 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     3661 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     3721 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     3781 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     3841 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     3901 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     3961 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     4021 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     4081 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     4141 gaaagttgga acctcttacg tgccgatcaa cgtctcattg atacctgaaa caaaacccat
     4201 cgtacggcca aggaagtctc caataactgt gatccaccac aagcgccagg gttttcccag
     4261 tcacgacgtt gtaaaacgac ggccagtcat gcataatccg cacgcatctg gaataaggaa
     4321 gtgccattcc gcctgacct
//
